Lack of humoral response against Helicobacter pylori peptides homologous to human ZnT8 in Hashimoto’s thyroiditis patients
Introduction: The Helicobacter pylori (HP) reinfection rate seems to be higher in developing countries than in developed ones. An increased seroprevalence of HP has also been reported in patients with type 1 diabetes (T1D) and Hashimoto’s thyroiditis (HT). Mycobacterium avium subsp. paratuberculosis...
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The Journal of Infection in Developing Countries
2015-07-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/6284 |
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| author | Speranza Masala Davide Cossu Magdalena Niegowska Giuseppe Mameli Daniela Paccagnini Leonardo A Sechi |
| author_facet | Speranza Masala Davide Cossu Magdalena Niegowska Giuseppe Mameli Daniela Paccagnini Leonardo A Sechi |
| author_sort | Speranza Masala |
| collection | DOAJ |
| description | Introduction: The Helicobacter pylori (HP) reinfection rate seems to be higher in developing countries than in developed ones. An increased seroprevalence of HP has also been reported in patients with type 1 diabetes (T1D) and Hashimoto’s thyroiditis (HT). Mycobacterium avium subsp. paratuberculosis (MAP) has been linked to both T1D and HT. Quite a few lines of evidence indicate that autoantibodies against several epitopes belonging to human zinc transporter 8 (ZnT8) cross-recognize the homologous MAP3865c epitopes in both T1D and HT patients. HP may play a role in HT disease, most likely acting through a molecular mimicry mechanism that targets ZnT8 as reported for MAP and the two autoimmune diseases.
Methodology: An enzyme-linked immunosorbent assay (ELISA) has been developed for the detection of antibodies against several epitopes deriving from HP proteins, which are highly homologous to the immunodominant ZnT8 peptides previously identified: ZnT8178–186 and ZnT8186–194.
Results: None of the HP peptides tested were significantly recognized when the humoral responses of 92 HT patients and 91 healthy volunteers were analyzed.
Conclusions: These findings do not support a triggering role for HP (through ZnT8 mimicking) in HT. If a molecular mimicry phenomenon is taking place, it involves a different self-antigen. Moreover, the negative outcome of the experiments performed stresses the fact that sharing stretches of sequence homology is relevant, but not enough to trigger an antibody-mediated cross-recognition.
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| format | Article |
| id | doaj-art-bfb5a266b91846f4a95c17b3e5d5985a |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2015-07-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-bfb5a266b91846f4a95c17b3e5d5985a2025-08-20T02:27:12ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802015-07-0190610.3855/jidc.6284Lack of humoral response against Helicobacter pylori peptides homologous to human ZnT8 in Hashimoto’s thyroiditis patientsSperanza Masala0Davide Cossu1Magdalena Niegowska2Giuseppe Mameli3Daniela Paccagnini4Leonardo A Sechi5University of Sassari, Sassari, ItalyUniversity of Sassari, Sassari, ItalyUniversity of Sassari, Sassari, ItalyUniversity of Sassari, Sassari, ItalyUniversity of Sassari, Sassari, ItalyUniversity of Sassari, Sassari, ItalyIntroduction: The Helicobacter pylori (HP) reinfection rate seems to be higher in developing countries than in developed ones. An increased seroprevalence of HP has also been reported in patients with type 1 diabetes (T1D) and Hashimoto’s thyroiditis (HT). Mycobacterium avium subsp. paratuberculosis (MAP) has been linked to both T1D and HT. Quite a few lines of evidence indicate that autoantibodies against several epitopes belonging to human zinc transporter 8 (ZnT8) cross-recognize the homologous MAP3865c epitopes in both T1D and HT patients. HP may play a role in HT disease, most likely acting through a molecular mimicry mechanism that targets ZnT8 as reported for MAP and the two autoimmune diseases. Methodology: An enzyme-linked immunosorbent assay (ELISA) has been developed for the detection of antibodies against several epitopes deriving from HP proteins, which are highly homologous to the immunodominant ZnT8 peptides previously identified: ZnT8178–186 and ZnT8186–194. Results: None of the HP peptides tested were significantly recognized when the humoral responses of 92 HT patients and 91 healthy volunteers were analyzed. Conclusions: These findings do not support a triggering role for HP (through ZnT8 mimicking) in HT. If a molecular mimicry phenomenon is taking place, it involves a different self-antigen. Moreover, the negative outcome of the experiments performed stresses the fact that sharing stretches of sequence homology is relevant, but not enough to trigger an antibody-mediated cross-recognition. https://jidc.org/index.php/journal/article/view/6284zinc transporter 8Hashimoto’s thyroiditisHelicobacter pylori |
| spellingShingle | Speranza Masala Davide Cossu Magdalena Niegowska Giuseppe Mameli Daniela Paccagnini Leonardo A Sechi Lack of humoral response against Helicobacter pylori peptides homologous to human ZnT8 in Hashimoto’s thyroiditis patients Journal of Infection in Developing Countries zinc transporter 8 Hashimoto’s thyroiditis Helicobacter pylori |
| title | Lack of humoral response against Helicobacter pylori peptides homologous to human ZnT8 in Hashimoto’s thyroiditis patients |
| title_full | Lack of humoral response against Helicobacter pylori peptides homologous to human ZnT8 in Hashimoto’s thyroiditis patients |
| title_fullStr | Lack of humoral response against Helicobacter pylori peptides homologous to human ZnT8 in Hashimoto’s thyroiditis patients |
| title_full_unstemmed | Lack of humoral response against Helicobacter pylori peptides homologous to human ZnT8 in Hashimoto’s thyroiditis patients |
| title_short | Lack of humoral response against Helicobacter pylori peptides homologous to human ZnT8 in Hashimoto’s thyroiditis patients |
| title_sort | lack of humoral response against helicobacter pylori peptides homologous to human znt8 in hashimoto s thyroiditis patients |
| topic | zinc transporter 8 Hashimoto’s thyroiditis Helicobacter pylori |
| url | https://jidc.org/index.php/journal/article/view/6284 |
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