Glucocorticoid receptor expression in human bronchial epithelial cells: effects of smoking and COPD
Previously, we found that inflammatory mediators modulated the number and binding affinity of glucocorticoid receptors (GR) in human bronchial epithelial cell lines . In this study we investigated whether smoking and chronic obstructive pulmonary disease (COPD), both characterized by airway inflamma...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
1998-01-01
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| Series: | Mediators of Inflammation |
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| Online Access: | http://dx.doi.org/10.1080/09629359890965 |
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| _version_ | 1832561731432349696 |
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| author | M. M. Verheggen P. W. C. Adriaansen-Soeting C. A. Berrevoets P. Th. W. van Hal A. O. Brinkmann H. C. Hoogsteden M. A. Versnel |
| author_facet | M. M. Verheggen P. W. C. Adriaansen-Soeting C. A. Berrevoets P. Th. W. van Hal A. O. Brinkmann H. C. Hoogsteden M. A. Versnel |
| author_sort | M. M. Verheggen |
| collection | DOAJ |
| description | Previously, we found that inflammatory mediators modulated the number and binding affinity of glucocorticoid receptors (GR) in human bronchial epithelial cell lines . In this study we investigated whether smoking and chronic obstructive pulmonary disease (COPD), both characterized by airway inflammation with increased levels of inflammatory mediators , affect GR characteristics in cultured human bronchial
epithelial cells (HBEC). A statisticallys ignificant difference was found be tween the dissociation constant
(Kd) values in HBEC from smoking (Kd = 0.98 ± 0.08 nM; n=6) and nonsmoking controls (Kd = 0.76 ± 0.10 nM, p=0.03; n=5), but no significant difference was found between the mean number of binding sites . Our results are the first indication that cultured HBEC from smokers possess GR with a lower binding affinity. This may result from the in flammation found in the airways from smokers . Further more, these results provide furthere vidence that the bronchial
epithelium may be an actual target for inhaled glucocor ticoid therapy. |
| format | Article |
| id | doaj-art-bfb23d4362d54f39955cf36478ac050d |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1998-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-bfb23d4362d54f39955cf36478ac050d2025-02-03T01:24:27ZengWileyMediators of Inflammation0962-93511466-18611998-01-017427528110.1080/09629359890965Glucocorticoid receptor expression in human bronchial epithelial cells: effects of smoking and COPDM. M. Verheggen0P. W. C. Adriaansen-Soeting1C. A. Berrevoets2P. Th. W. van Hal3A. O. Brinkmann4H. C. Hoogsteden5M. A. Versnel6Department of Immunology, Erasmus University and University Hospital Rotterdam-Dijkzigt, PO Box 1738, Rotterdam 3000 DR, The NetherlandsDepartment of Immunology, Erasmus University and University Hospital Rotterdam-Dijkzigt, PO Box 1738, Rotterdam 3000 DR, The NetherlandsDepartment of Endocrinology and Reproduction, Erasmus University and University Hospital Rotterdam-Dijkzigt, PO Box 1738, Rotterdam 3000 DR, The NetherlandsDepartment of Immunology, Erasmus University and University Hospital Rotterdam-Dijkzigt, PO Box 1738, Rotterdam 3000 DR, The NetherlandsDepartment of Endocrinology and Reproduction, Erasmus University and University Hospital Rotterdam-Dijkzigt, PO Box 1738, Rotterdam 3000 DR, The NetherlandsDepartment of Pulmonary Medicine, Erasmus University and University Hospital Rotterdam-Dijkzigt, PO Box 1738, Rotterdam 3000 DR, The NetherlandsDepartment of Immunology, Erasmus University and University Hospital Rotterdam-Dijkzigt, PO Box 1738, Rotterdam 3000 DR, The NetherlandsPreviously, we found that inflammatory mediators modulated the number and binding affinity of glucocorticoid receptors (GR) in human bronchial epithelial cell lines . In this study we investigated whether smoking and chronic obstructive pulmonary disease (COPD), both characterized by airway inflammation with increased levels of inflammatory mediators , affect GR characteristics in cultured human bronchial epithelial cells (HBEC). A statisticallys ignificant difference was found be tween the dissociation constant (Kd) values in HBEC from smoking (Kd = 0.98 ± 0.08 nM; n=6) and nonsmoking controls (Kd = 0.76 ± 0.10 nM, p=0.03; n=5), but no significant difference was found between the mean number of binding sites . Our results are the first indication that cultured HBEC from smokers possess GR with a lower binding affinity. This may result from the in flammation found in the airways from smokers . Further more, these results provide furthere vidence that the bronchial epithelium may be an actual target for inhaled glucocor ticoid therapy.http://dx.doi.org/10.1080/09629359890965Glucocorticoid receptorsHuman bronchial epithelial cellsChronic obstructive pulmonary disease Smoking. |
| spellingShingle | M. M. Verheggen P. W. C. Adriaansen-Soeting C. A. Berrevoets P. Th. W. van Hal A. O. Brinkmann H. C. Hoogsteden M. A. Versnel Glucocorticoid receptor expression in human bronchial epithelial cells: effects of smoking and COPD Mediators of Inflammation Glucocorticoid receptors Human bronchial epithelial cells Chronic obstructive pulmonary disease Smoking. |
| title | Glucocorticoid receptor expression in human bronchial epithelial cells: effects
of smoking and COPD |
| title_full | Glucocorticoid receptor expression in human bronchial epithelial cells: effects
of smoking and COPD |
| title_fullStr | Glucocorticoid receptor expression in human bronchial epithelial cells: effects
of smoking and COPD |
| title_full_unstemmed | Glucocorticoid receptor expression in human bronchial epithelial cells: effects
of smoking and COPD |
| title_short | Glucocorticoid receptor expression in human bronchial epithelial cells: effects
of smoking and COPD |
| title_sort | glucocorticoid receptor expression in human bronchial epithelial cells effects of smoking and copd |
| topic | Glucocorticoid receptors Human bronchial epithelial cells Chronic obstructive pulmonary disease Smoking. |
| url | http://dx.doi.org/10.1080/09629359890965 |
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