Complex class 1 integrons harboring CTX-M-2-encoding genes in clinical Enterobacteriaceae from a hospital in Brazil
Introduction: CTX-M enzymes are the most prevalent extended-spectrum beta-lactamases (ESBLs) in Brazil and around the world. The spread of CTX-M lies in their ability to be mobilized by insertion sequences and integrons. This study aimed to identify the mobile genetic structures associated with blaC...
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The Journal of Infection in Developing Countries
2015-08-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/6241 |
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| author | Milena Dropa Livia Carminato Balsalobre Nilton Lincopan Glavur Rogério Matté Maria Helena Matté |
| author_facet | Milena Dropa Livia Carminato Balsalobre Nilton Lincopan Glavur Rogério Matté Maria Helena Matté |
| author_sort | Milena Dropa |
| collection | DOAJ |
| description | Introduction: CTX-M enzymes are the most prevalent extended-spectrum beta-lactamases (ESBLs) in Brazil and around the world. The spread of CTX-M lies in their ability to be mobilized by insertion sequences and integrons. This study aimed to identify the mobile genetic structures associated with blaCTX-M genes from clinical Enterobacteriaceae strains.
Methodology: Twenty-eight clinical non-clonal Enterobacteriaceae were screened by PCR for the presence of blaCTX-M genes and class 1 integrase (int1), and for the association of blaCTX-M with class 1 integrons. Plasmid incompatibility groups were assessed by PBRT. Wild-type plasmids were transformed into electrocompetent E. coli, and the S1-PFGE technique was used to verify the presence of high-molecular-weight plasmids in both wild-type strains and E. coli transformants.
Results: Sequencing showed that strains carried blaCTX-M-2 (n = 25) and blaCTX-M-59 (n = 3) genes inserted into the 3’-end of complex class 1 integrons. Thirteen strains also carried blaTEM and blaSHV genes. CTX-M-2/59-containing complex class 1 integrons were also present in E. coli transformants. The most frequent Inc groups were IncA/C (n = 10) and IncF (n = 8). Heavy plasmids were observed in both wild-type strains and E. coli transformants.
Conclusions: The presence of the same blaCTX-M-2-group-containing genetic structure in seven Enterobacteriaceae species isolated at seven hospital wards shows the great mobility potential of complex class 1 integrons. Also, this is the first report of TEM-15, SHV-45, and SHV-55 in Latin America. The genetic environment of blaCTX-M-2 accounts for their maintenance and spread among Gram-negative bacteria.
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| format | Article |
| id | doaj-art-bfb22c9ef3584562bdc53602f0412efc |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2015-08-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-bfb22c9ef3584562bdc53602f0412efc2025-08-20T03:52:42ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802015-08-0190810.3855/jidc.6241Complex class 1 integrons harboring CTX-M-2-encoding genes in clinical Enterobacteriaceae from a hospital in BrazilMilena Dropa0Livia Carminato Balsalobre1Nilton Lincopan2Glavur Rogério Matté3Maria Helena Matté4School of Public Health, University of São Paulo, São Paulo, BrazilSchool of Public Health, University of São Paulo, São Paulo, BrazilInstitute of Biomedical Sciences, University of São Paulo, Butantã, BrazilSchool of Public Health, University of São Paulo, São Paulo, BrazilSchool of Public Health, University of São Paulo, São Paulo, BrazilIntroduction: CTX-M enzymes are the most prevalent extended-spectrum beta-lactamases (ESBLs) in Brazil and around the world. The spread of CTX-M lies in their ability to be mobilized by insertion sequences and integrons. This study aimed to identify the mobile genetic structures associated with blaCTX-M genes from clinical Enterobacteriaceae strains. Methodology: Twenty-eight clinical non-clonal Enterobacteriaceae were screened by PCR for the presence of blaCTX-M genes and class 1 integrase (int1), and for the association of blaCTX-M with class 1 integrons. Plasmid incompatibility groups were assessed by PBRT. Wild-type plasmids were transformed into electrocompetent E. coli, and the S1-PFGE technique was used to verify the presence of high-molecular-weight plasmids in both wild-type strains and E. coli transformants. Results: Sequencing showed that strains carried blaCTX-M-2 (n = 25) and blaCTX-M-59 (n = 3) genes inserted into the 3’-end of complex class 1 integrons. Thirteen strains also carried blaTEM and blaSHV genes. CTX-M-2/59-containing complex class 1 integrons were also present in E. coli transformants. The most frequent Inc groups were IncA/C (n = 10) and IncF (n = 8). Heavy plasmids were observed in both wild-type strains and E. coli transformants. Conclusions: The presence of the same blaCTX-M-2-group-containing genetic structure in seven Enterobacteriaceae species isolated at seven hospital wards shows the great mobility potential of complex class 1 integrons. Also, this is the first report of TEM-15, SHV-45, and SHV-55 in Latin America. The genetic environment of blaCTX-M-2 accounts for their maintenance and spread among Gram-negative bacteria. https://jidc.org/index.php/journal/article/view/6241complex class 1 integronCTX-M-2CTX-M-59EnterobacteriaceaeBrazil |
| spellingShingle | Milena Dropa Livia Carminato Balsalobre Nilton Lincopan Glavur Rogério Matté Maria Helena Matté Complex class 1 integrons harboring CTX-M-2-encoding genes in clinical Enterobacteriaceae from a hospital in Brazil Journal of Infection in Developing Countries complex class 1 integron CTX-M-2 CTX-M-59 Enterobacteriaceae Brazil |
| title | Complex class 1 integrons harboring CTX-M-2-encoding genes in clinical Enterobacteriaceae from a hospital in Brazil |
| title_full | Complex class 1 integrons harboring CTX-M-2-encoding genes in clinical Enterobacteriaceae from a hospital in Brazil |
| title_fullStr | Complex class 1 integrons harboring CTX-M-2-encoding genes in clinical Enterobacteriaceae from a hospital in Brazil |
| title_full_unstemmed | Complex class 1 integrons harboring CTX-M-2-encoding genes in clinical Enterobacteriaceae from a hospital in Brazil |
| title_short | Complex class 1 integrons harboring CTX-M-2-encoding genes in clinical Enterobacteriaceae from a hospital in Brazil |
| title_sort | complex class 1 integrons harboring ctx m 2 encoding genes in clinical enterobacteriaceae from a hospital in brazil |
| topic | complex class 1 integron CTX-M-2 CTX-M-59 Enterobacteriaceae Brazil |
| url | https://jidc.org/index.php/journal/article/view/6241 |
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