Isolation and Characterization of Multipotent Turkey Tendon-Derived Stem Cells
Tendon injuries are among the most common and severe hand injuries with a high demand for functional recovery. Stem cells have been identified and isolated from different species and a variety of tissues for the sake of regenerative medicine. Recently, turkey has been suggested as a potential new la...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2018/3697971 |
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| author | Qian Liu Yaxi Zhu Peter C. Amadio Steven L. Moran Anne Gingery Chunfeng Zhao |
| author_facet | Qian Liu Yaxi Zhu Peter C. Amadio Steven L. Moran Anne Gingery Chunfeng Zhao |
| author_sort | Qian Liu |
| collection | DOAJ |
| description | Tendon injuries are among the most common and severe hand injuries with a high demand for functional recovery. Stem cells have been identified and isolated from different species and a variety of tissues for the sake of regenerative medicine. Recently, turkey has been suggested as a potential new large animal model for flexor tendon-related research. However, turkey tissue-specific stem cells have not been investigated. Here, we presented the isolation and verification of tendon-derived stem cells (TDSCs) from 6- to 8-month-old heritage-breed turkey. TDSCs were isolated from turkey flexor tendon by plating nucleated cells at the determined optimal density. Approximately 4% of the nucleated cells demonstrated clonogenicity, high proliferation rate, and trilineage differentiation potential after induction culturing. These cells expressed surface antigens CD90, CD105, and CD44, but did not express CD45. There was a high level of gene expression of tenogenic markers in TDSCs, including mohawk, collagen type I, tenascin C, and elastin. Turkey TDSCs also expressed transcription factors PouV, Nanog, and Sox2, which are critically involved in the regulation of stemness. The successful isolation of tendon-derived stem cells from turkey was beneficial for future studies in tendon tissue engineering and would help in the development of new treatment for tendon diseases using this novel animal model. |
| format | Article |
| id | doaj-art-bfad640809a7405a8f0f6d68b88665e7 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-bfad640809a7405a8f0f6d68b88665e72025-02-03T07:25:38ZengWileyStem Cells International1687-966X1687-96782018-01-01201810.1155/2018/36979713697971Isolation and Characterization of Multipotent Turkey Tendon-Derived Stem CellsQian Liu0Yaxi Zhu1Peter C. Amadio2Steven L. Moran3Anne Gingery4Chunfeng Zhao5Department of Orthopedic Surgery, Mayo Clinic, Rochester, MN, USADepartment of Molecular Medicine, Mayo Clinic, Rochester, MN, USADepartment of Orthopedic Surgery, Mayo Clinic, Rochester, MN, USADepartment of Orthopedic Surgery, Mayo Clinic, Rochester, MN, USADepartment of Orthopedic Surgery, Mayo Clinic, Rochester, MN, USADepartment of Orthopedic Surgery, Mayo Clinic, Rochester, MN, USATendon injuries are among the most common and severe hand injuries with a high demand for functional recovery. Stem cells have been identified and isolated from different species and a variety of tissues for the sake of regenerative medicine. Recently, turkey has been suggested as a potential new large animal model for flexor tendon-related research. However, turkey tissue-specific stem cells have not been investigated. Here, we presented the isolation and verification of tendon-derived stem cells (TDSCs) from 6- to 8-month-old heritage-breed turkey. TDSCs were isolated from turkey flexor tendon by plating nucleated cells at the determined optimal density. Approximately 4% of the nucleated cells demonstrated clonogenicity, high proliferation rate, and trilineage differentiation potential after induction culturing. These cells expressed surface antigens CD90, CD105, and CD44, but did not express CD45. There was a high level of gene expression of tenogenic markers in TDSCs, including mohawk, collagen type I, tenascin C, and elastin. Turkey TDSCs also expressed transcription factors PouV, Nanog, and Sox2, which are critically involved in the regulation of stemness. The successful isolation of tendon-derived stem cells from turkey was beneficial for future studies in tendon tissue engineering and would help in the development of new treatment for tendon diseases using this novel animal model.http://dx.doi.org/10.1155/2018/3697971 |
| spellingShingle | Qian Liu Yaxi Zhu Peter C. Amadio Steven L. Moran Anne Gingery Chunfeng Zhao Isolation and Characterization of Multipotent Turkey Tendon-Derived Stem Cells Stem Cells International |
| title | Isolation and Characterization of Multipotent Turkey Tendon-Derived Stem Cells |
| title_full | Isolation and Characterization of Multipotent Turkey Tendon-Derived Stem Cells |
| title_fullStr | Isolation and Characterization of Multipotent Turkey Tendon-Derived Stem Cells |
| title_full_unstemmed | Isolation and Characterization of Multipotent Turkey Tendon-Derived Stem Cells |
| title_short | Isolation and Characterization of Multipotent Turkey Tendon-Derived Stem Cells |
| title_sort | isolation and characterization of multipotent turkey tendon derived stem cells |
| url | http://dx.doi.org/10.1155/2018/3697971 |
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