The Involvement of Notch1-RBP-Jk/Msx2 Signaling Pathway in Aortic Calcification of Diabetic Nephropathy Rats
Background. This study explored the changes in expression of vascular smooth muscle cell (VSMC) markers and osteogenic markers, as well as the involvement of Notch1-RBP-Jk/Msx2 pathway in a rat model of diabetic nephropathy (DN) with vascular calcification. Methods. A rat model of DN with concomitan...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2017/8968523 |
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| author | Caipan Gong Li Li Chunmei Qin Weihua Wu Qi Liu Ying Li Linwang Gan Santao Ou |
| author_facet | Caipan Gong Li Li Chunmei Qin Weihua Wu Qi Liu Ying Li Linwang Gan Santao Ou |
| author_sort | Caipan Gong |
| collection | DOAJ |
| description | Background. This study explored the changes in expression of vascular smooth muscle cell (VSMC) markers and osteogenic markers, as well as the involvement of Notch1-RBP-Jk/Msx2 pathway in a rat model of diabetic nephropathy (DN) with vascular calcification. Methods. A rat model of DN with concomitant vascular calcification was created by intraperitoneal injection of streptozotocin followed by administration of vitamin D3 and nicotine. Biochemical analysis and histological examination of aortic tissue were performed. VSMC markers and osteogenic markers as well as target molecules in Notch1-RBP-Jk/Msx2 were determined by quantitative real-time polymerase chain reaction and immunohistochemical analysis. Results. Serum calcium and phosphorus levels were significantly increased in model rats as compared to that in normal controls. Diabetic rats with vascular calcification exhibited mineral deposits in aortic intima-media accompanied by decreased expression of VSMC markers and increased expression of osteogenic markers. Notch1, RBP-Jk, Msx2, Jagged1, and N1-ICD were barely expressed in the aortic wall of normal rats. In contrast, these were significantly increased in the model group at all time points (8, 12, and 16 weeks), as compared to that in the normal rats. Conclusion. Activation of the Notch1-RBP-Jk/Msx2 signaling pathway may be involved in the development and progression of vascular calcification in DN. |
| format | Article |
| id | doaj-art-bfab158b937847c689e0cf676349803e |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-bfab158b937847c689e0cf676349803e2025-02-03T01:27:52ZengWileyJournal of Diabetes Research2314-67452314-67532017-01-01201710.1155/2017/89685238968523The Involvement of Notch1-RBP-Jk/Msx2 Signaling Pathway in Aortic Calcification of Diabetic Nephropathy RatsCaipan Gong0Li Li1Chunmei Qin2Weihua Wu3Qi Liu4Ying Li5Linwang Gan6Santao Ou7Department of Nephrology, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, ChinaDepartment of Nephrology, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, ChinaDepartment of Nephrology, Luzhou People’s Hospital, Luzhou, Sichuan 646000, ChinaDepartment of Nephrology, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, ChinaDepartment of Nephrology, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, ChinaDepartment of Nephrology, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, ChinaDepartment of Nephrology, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, ChinaDepartment of Nephrology, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, ChinaBackground. This study explored the changes in expression of vascular smooth muscle cell (VSMC) markers and osteogenic markers, as well as the involvement of Notch1-RBP-Jk/Msx2 pathway in a rat model of diabetic nephropathy (DN) with vascular calcification. Methods. A rat model of DN with concomitant vascular calcification was created by intraperitoneal injection of streptozotocin followed by administration of vitamin D3 and nicotine. Biochemical analysis and histological examination of aortic tissue were performed. VSMC markers and osteogenic markers as well as target molecules in Notch1-RBP-Jk/Msx2 were determined by quantitative real-time polymerase chain reaction and immunohistochemical analysis. Results. Serum calcium and phosphorus levels were significantly increased in model rats as compared to that in normal controls. Diabetic rats with vascular calcification exhibited mineral deposits in aortic intima-media accompanied by decreased expression of VSMC markers and increased expression of osteogenic markers. Notch1, RBP-Jk, Msx2, Jagged1, and N1-ICD were barely expressed in the aortic wall of normal rats. In contrast, these were significantly increased in the model group at all time points (8, 12, and 16 weeks), as compared to that in the normal rats. Conclusion. Activation of the Notch1-RBP-Jk/Msx2 signaling pathway may be involved in the development and progression of vascular calcification in DN.http://dx.doi.org/10.1155/2017/8968523 |
| spellingShingle | Caipan Gong Li Li Chunmei Qin Weihua Wu Qi Liu Ying Li Linwang Gan Santao Ou The Involvement of Notch1-RBP-Jk/Msx2 Signaling Pathway in Aortic Calcification of Diabetic Nephropathy Rats Journal of Diabetes Research |
| title | The Involvement of Notch1-RBP-Jk/Msx2 Signaling Pathway in Aortic Calcification of Diabetic Nephropathy Rats |
| title_full | The Involvement of Notch1-RBP-Jk/Msx2 Signaling Pathway in Aortic Calcification of Diabetic Nephropathy Rats |
| title_fullStr | The Involvement of Notch1-RBP-Jk/Msx2 Signaling Pathway in Aortic Calcification of Diabetic Nephropathy Rats |
| title_full_unstemmed | The Involvement of Notch1-RBP-Jk/Msx2 Signaling Pathway in Aortic Calcification of Diabetic Nephropathy Rats |
| title_short | The Involvement of Notch1-RBP-Jk/Msx2 Signaling Pathway in Aortic Calcification of Diabetic Nephropathy Rats |
| title_sort | involvement of notch1 rbp jk msx2 signaling pathway in aortic calcification of diabetic nephropathy rats |
| url | http://dx.doi.org/10.1155/2017/8968523 |
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