Hydrogen gas inhalation prior to high-intensity training reduces attenuation of nitric oxide bioavailability in male rugby players

Background Inhalation of hydrogen gas (H2) as an antioxidant supplement may alleviate exercise-induced oxidative damage and protect post-exercise hydrogen peroxide signaling, which may help mediate beneficial exercise adaptation. The aims of this study were to determine the effects of H2 inhalation...

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Main Authors: Yuqi Zhao, Chaoqun Li, Shi Zhou, Zhiguang Xu, Xin Huang, Li Wen
Format: Article
Language:English
Published: PeerJ Inc. 2024-12-01
Series:PeerJ
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Online Access:https://peerj.com/articles/18503.pdf
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author Yuqi Zhao
Chaoqun Li
Shi Zhou
Zhiguang Xu
Xin Huang
Li Wen
author_facet Yuqi Zhao
Chaoqun Li
Shi Zhou
Zhiguang Xu
Xin Huang
Li Wen
author_sort Yuqi Zhao
collection DOAJ
description Background Inhalation of hydrogen gas (H2) as an antioxidant supplement may alleviate exercise-induced oxidative damage and protect post-exercise hydrogen peroxide signaling, which may help mediate beneficial exercise adaptation. The aims of this study were to determine the effects of H2 inhalation on plasma nitric oxide (NO) level and its synthesis precursor in professional athletes. Methods A randomized, placebo-controlled, double-blind, crossover trial was conducted with professional male rugby players for 3 weeks. Participants underwent 1 week of H2 supplementation and 1 week of placebo treatment prior to daily sessions of high-intensity exercise training, separated by 1 week of low-intensity training as a washout. Results Two-way (supplementation and time) repeated-measures analyses of variance showed that NO, L-arginine, and tetrahydrobiopterin levels in the H2 inhalation group were significantly higher than those in the placebo group after exercise (D6) and remained higher after 24 h of rest (D7). Levels of hydroxydeoxyguanosine and interleukin 6 were lower in the H2 inhalation week than in the placebo week on D6 and D7. In addition, total antioxidant levels were significantly higher with H2 inhalation than with placebo. Significance These results suggest that H2 inhalation helps to maintain NO signaling after exercise and to alleviate inflammation and oxidative stress induced by high-intensity exercise training in professional athletes.
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spelling doaj-art-bfa7b637499e48aab660efeaee1590502025-08-20T01:58:04ZengPeerJ Inc.PeerJ2167-83592024-12-0112e1850310.7717/peerj.18503Hydrogen gas inhalation prior to high-intensity training reduces attenuation of nitric oxide bioavailability in male rugby playersYuqi Zhao0Chaoqun Li1Shi Zhou2Zhiguang Xu3Xin Huang4Li Wen5School of Sports and Health, Nanjing Sport Institute, Nanjing, ChinaSchool of Kinesiology, Shanghai University of Sport, Shanghai, ChinaFaculty of Health, Southern Cross University, Lismore, AustraliaSchool of Social Sports and Health Sciences, Tianjin University of Sport, Tianjin, ChinaSchool of Social Sports and Health Sciences, Tianjin University of Sport, Tianjin, ChinaSchool of Sports and Health, Nanjing Sport Institute, Nanjing, ChinaBackground Inhalation of hydrogen gas (H2) as an antioxidant supplement may alleviate exercise-induced oxidative damage and protect post-exercise hydrogen peroxide signaling, which may help mediate beneficial exercise adaptation. The aims of this study were to determine the effects of H2 inhalation on plasma nitric oxide (NO) level and its synthesis precursor in professional athletes. Methods A randomized, placebo-controlled, double-blind, crossover trial was conducted with professional male rugby players for 3 weeks. Participants underwent 1 week of H2 supplementation and 1 week of placebo treatment prior to daily sessions of high-intensity exercise training, separated by 1 week of low-intensity training as a washout. Results Two-way (supplementation and time) repeated-measures analyses of variance showed that NO, L-arginine, and tetrahydrobiopterin levels in the H2 inhalation group were significantly higher than those in the placebo group after exercise (D6) and remained higher after 24 h of rest (D7). Levels of hydroxydeoxyguanosine and interleukin 6 were lower in the H2 inhalation week than in the placebo week on D6 and D7. In addition, total antioxidant levels were significantly higher with H2 inhalation than with placebo. Significance These results suggest that H2 inhalation helps to maintain NO signaling after exercise and to alleviate inflammation and oxidative stress induced by high-intensity exercise training in professional athletes.https://peerj.com/articles/18503.pdfAdaptationHydrogenNitric oxideOxidative stressSports training
spellingShingle Yuqi Zhao
Chaoqun Li
Shi Zhou
Zhiguang Xu
Xin Huang
Li Wen
Hydrogen gas inhalation prior to high-intensity training reduces attenuation of nitric oxide bioavailability in male rugby players
PeerJ
Adaptation
Hydrogen
Nitric oxide
Oxidative stress
Sports training
title Hydrogen gas inhalation prior to high-intensity training reduces attenuation of nitric oxide bioavailability in male rugby players
title_full Hydrogen gas inhalation prior to high-intensity training reduces attenuation of nitric oxide bioavailability in male rugby players
title_fullStr Hydrogen gas inhalation prior to high-intensity training reduces attenuation of nitric oxide bioavailability in male rugby players
title_full_unstemmed Hydrogen gas inhalation prior to high-intensity training reduces attenuation of nitric oxide bioavailability in male rugby players
title_short Hydrogen gas inhalation prior to high-intensity training reduces attenuation of nitric oxide bioavailability in male rugby players
title_sort hydrogen gas inhalation prior to high intensity training reduces attenuation of nitric oxide bioavailability in male rugby players
topic Adaptation
Hydrogen
Nitric oxide
Oxidative stress
Sports training
url https://peerj.com/articles/18503.pdf
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