The synergistic antibacterial effects of photothermal and enzymatic catalysis mediated by Pd/ZIF-8 nanozyme
Objective To explore the synergistic antibacterial performance of photothermal and enzymatic catalysis mediated by Pd/ZIF-8 nanozyme, and assess its potential for biomedical applications. Methods The Pd/ZIF-8 nanozyme was prepared by the hydrothermal method, and its structure was characterized by tr...
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Editorial Office of Journal of Guangxi Medical University
2025-06-01
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| Series: | Guangxi Yike Daxue xuebao |
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| Online Access: | https://journal.gxmu.edu.cn/article/doi/10.16190/j.cnki.45-1211/r.2025.03.009 |
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| author | Miao MO Zhangrui HUANG Xin ZHAO Jingping ZHONG |
| author_facet | Miao MO Zhangrui HUANG Xin ZHAO Jingping ZHONG |
| author_sort | Miao MO |
| collection | DOAJ |
| description | Objective To explore the synergistic antibacterial performance of photothermal and enzymatic catalysis mediated by Pd/ZIF-8 nanozyme, and assess its potential for biomedical applications. Methods The Pd/ZIF-8 nanozyme was prepared by the hydrothermal method, and its structure was characterized by transmission electron microscopy (TEM) and X-ray diffraction (XRD). The photothermal conversion performance of the Pd/ZIF-8 nanozyme and its ability to mimic the activities of peroxidase (POD) and glutathione peroxidase (GPx) were evaluated. The cell counting kit-8 (CCK-8) method was used to detect the toxicity of Pd/ZIF-8 to human dermal fibroblasts (HDF-a), and the erythrocyte hemolysis test was used to detect its blood compatibility. The antibacterial performance of Pd/ZIF-8 against Gram-negative bacteria (Escherichia coli, E. coli) and Gram-positive bacteria (methicillin-resistant Staphylococcus aureus, MRSA) under the irradiation of 808 nm near-infrared light was detected. Results TEM and XRD analyses confirmed the successful synthesis of the Pd/ZIF-8 nanozyme. The Pd/ZIF-8 nanozyme exhibited good photothermal performance with a photothermal conversion rate of 65.3% and good stability. Meanwhile, the Pd/ZIF-8 nanozyme exhibited excellent dual enzyme-like activities (POD and GPx). The results of cytotoxicity and hemolysis tests indicated that Pd/ZIF-8 had good biocompatibility. Under near infrared light irradiation, the antibacterial rates of Pd/ZIF-8 against E. coli and MRSA were 97.3% and 99.41%, respectively (P < 0.0001). Conclusion The Pd/ZIF-8 nanozyme has the ability to mediate photothermal and enzymatic promotion to achieve synergistic antimicrobial properties. This nanomaterial shows promising application prospects in the field of anti-infection treatment and provides important ideas for the development of new antibacterial agents. |
| format | Article |
| id | doaj-art-bfa45af28cdd4311874ccebe800c4bfe |
| institution | Kabale University |
| issn | 1005-930X |
| language | zho |
| publishDate | 2025-06-01 |
| publisher | Editorial Office of Journal of Guangxi Medical University |
| record_format | Article |
| series | Guangxi Yike Daxue xuebao |
| spelling | doaj-art-bfa45af28cdd4311874ccebe800c4bfe2025-08-20T03:29:21ZzhoEditorial Office of Journal of Guangxi Medical UniversityGuangxi Yike Daxue xuebao1005-930X2025-06-0142339540110.16190/j.cnki.45-1211/r.2025.03.009gxykdxxb-42-3-395The synergistic antibacterial effects of photothermal and enzymatic catalysis mediated by Pd/ZIF-8 nanozymeMiao MO0Zhangrui HUANG1Xin ZHAO2Jingping ZHONG3Collaborative Innovation Centre of Regenerative Medicine and Medical BioResource Development and Application Co-constructed by the Province and Ministry, Guangxi Medical University, Nanning 530021, ChinaGuangxi Engineering Center in Biomedical Materials for Tissue and Organ Regeneration, Guangxi Medical University, Nanning 530021, ChinaGuangxi Engineering Center in Biomedical Materials for Tissue and Organ Regeneration, Guangxi Medical University, Nanning 530021, ChinaGuangxi Engineering Center in Biomedical Materials for Tissue and Organ Regeneration, Guangxi Medical University, Nanning 530021, ChinaObjective To explore the synergistic antibacterial performance of photothermal and enzymatic catalysis mediated by Pd/ZIF-8 nanozyme, and assess its potential for biomedical applications. Methods The Pd/ZIF-8 nanozyme was prepared by the hydrothermal method, and its structure was characterized by transmission electron microscopy (TEM) and X-ray diffraction (XRD). The photothermal conversion performance of the Pd/ZIF-8 nanozyme and its ability to mimic the activities of peroxidase (POD) and glutathione peroxidase (GPx) were evaluated. The cell counting kit-8 (CCK-8) method was used to detect the toxicity of Pd/ZIF-8 to human dermal fibroblasts (HDF-a), and the erythrocyte hemolysis test was used to detect its blood compatibility. The antibacterial performance of Pd/ZIF-8 against Gram-negative bacteria (Escherichia coli, E. coli) and Gram-positive bacteria (methicillin-resistant Staphylococcus aureus, MRSA) under the irradiation of 808 nm near-infrared light was detected. Results TEM and XRD analyses confirmed the successful synthesis of the Pd/ZIF-8 nanozyme. The Pd/ZIF-8 nanozyme exhibited good photothermal performance with a photothermal conversion rate of 65.3% and good stability. Meanwhile, the Pd/ZIF-8 nanozyme exhibited excellent dual enzyme-like activities (POD and GPx). The results of cytotoxicity and hemolysis tests indicated that Pd/ZIF-8 had good biocompatibility. Under near infrared light irradiation, the antibacterial rates of Pd/ZIF-8 against E. coli and MRSA were 97.3% and 99.41%, respectively (P < 0.0001). Conclusion The Pd/ZIF-8 nanozyme has the ability to mediate photothermal and enzymatic promotion to achieve synergistic antimicrobial properties. This nanomaterial shows promising application prospects in the field of anti-infection treatment and provides important ideas for the development of new antibacterial agents.https://journal.gxmu.edu.cn/article/doi/10.16190/j.cnki.45-1211/r.2025.03.009nanozymenear-infrared lightantibacterial activityenzymatic catalysis |
| spellingShingle | Miao MO Zhangrui HUANG Xin ZHAO Jingping ZHONG The synergistic antibacterial effects of photothermal and enzymatic catalysis mediated by Pd/ZIF-8 nanozyme Guangxi Yike Daxue xuebao nanozyme near-infrared light antibacterial activity enzymatic catalysis |
| title | The synergistic antibacterial effects of photothermal and enzymatic catalysis mediated by Pd/ZIF-8 nanozyme |
| title_full | The synergistic antibacterial effects of photothermal and enzymatic catalysis mediated by Pd/ZIF-8 nanozyme |
| title_fullStr | The synergistic antibacterial effects of photothermal and enzymatic catalysis mediated by Pd/ZIF-8 nanozyme |
| title_full_unstemmed | The synergistic antibacterial effects of photothermal and enzymatic catalysis mediated by Pd/ZIF-8 nanozyme |
| title_short | The synergistic antibacterial effects of photothermal and enzymatic catalysis mediated by Pd/ZIF-8 nanozyme |
| title_sort | synergistic antibacterial effects of photothermal and enzymatic catalysis mediated by pd zif 8 nanozyme |
| topic | nanozyme near-infrared light antibacterial activity enzymatic catalysis |
| url | https://journal.gxmu.edu.cn/article/doi/10.16190/j.cnki.45-1211/r.2025.03.009 |
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