Mitochondrial DNA from archived tissue samples kept in formalin for forensic odontology studies
Background: Samples used for DNA isolation to be used for forensic odontology studies are often limited. The possibility to use tissue samples stored in formalin for a prolonged period, which contains nucleic acids of questionable quality, opens exciting possibilities for genetic and molecular biolo...
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| Format: | Article |
| Language: | English |
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Elsevier
2014-05-01
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| Series: | Journal of Oral Biology and Craniofacial Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212426814000384 |
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| author | Rahul Pandey Divya Mehrotra Pradnya Kowtal Abbas Ali Mahdi Rajiv Sarin |
| author_facet | Rahul Pandey Divya Mehrotra Pradnya Kowtal Abbas Ali Mahdi Rajiv Sarin |
| author_sort | Rahul Pandey |
| collection | DOAJ |
| description | Background: Samples used for DNA isolation to be used for forensic odontology studies are often limited. The possibility to use tissue samples stored in formalin for a prolonged period, which contains nucleic acids of questionable quality, opens exciting possibilities for genetic and molecular biology studies useful in speciality of forensic odontology. Aim: The present study defines substantial modification of existing protocols for total genomic isolation including mitochondrial DNA and proves the utility of such obtained mitochondrial DNA in microsatellite analyses. Methods: 50 dental tissue samples which were kept in neutral buffered formalin liquid bottles were taken for DNA isolation and subsequent analysis. For the isolation of total genomic DNA from tissue samples, a new protocol with substantial modifications from routine ones was adopted by us. Total genomic DNA from matched blood samples were extracted using standard phenol-chloroform extraction method. Results: Polymerase Chain Reaction and Sequencing of such extracted DNA samples for mitochondrial D loop region were successful and the results were comparable with DNA extracted from normal sources of samples. Conclusion: The present study reports for the first time that nucleic acids extracted from human dental tissue samples under prolonged formalin fixation times can be used for forensic odontology studies using the described methodology. |
| format | Article |
| id | doaj-art-bfa2f07e87d7478382f3eee103e99319 |
| institution | OA Journals |
| issn | 2212-4268 |
| language | English |
| publishDate | 2014-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Oral Biology and Craniofacial Research |
| spelling | doaj-art-bfa2f07e87d7478382f3eee103e993192025-08-20T02:02:02ZengElsevierJournal of Oral Biology and Craniofacial Research2212-42682014-05-014210911310.1016/j.jobcr.2014.08.004Mitochondrial DNA from archived tissue samples kept in formalin for forensic odontology studiesRahul Pandey0Divya Mehrotra1Pradnya Kowtal2Abbas Ali Mahdi3Rajiv Sarin4Department of Oral & Maxillofacial Surgery, King George's Medical University, Lucknow, Uttar Pradesh, IndiaDepartment of Oral & Maxillofacial Surgery, King George's Medical University, Lucknow, Uttar Pradesh, India; Corresponding author.ACTREC, Tata Memorial Centre, Mumbai, Maharashtra, IndiaDepartment of Biochemistry, King George's Medical University, Lucknow, Uttar Pradesh, IndiaACTREC, Tata Memorial Centre, Mumbai, Maharashtra, IndiaBackground: Samples used for DNA isolation to be used for forensic odontology studies are often limited. The possibility to use tissue samples stored in formalin for a prolonged period, which contains nucleic acids of questionable quality, opens exciting possibilities for genetic and molecular biology studies useful in speciality of forensic odontology. Aim: The present study defines substantial modification of existing protocols for total genomic isolation including mitochondrial DNA and proves the utility of such obtained mitochondrial DNA in microsatellite analyses. Methods: 50 dental tissue samples which were kept in neutral buffered formalin liquid bottles were taken for DNA isolation and subsequent analysis. For the isolation of total genomic DNA from tissue samples, a new protocol with substantial modifications from routine ones was adopted by us. Total genomic DNA from matched blood samples were extracted using standard phenol-chloroform extraction method. Results: Polymerase Chain Reaction and Sequencing of such extracted DNA samples for mitochondrial D loop region were successful and the results were comparable with DNA extracted from normal sources of samples. Conclusion: The present study reports for the first time that nucleic acids extracted from human dental tissue samples under prolonged formalin fixation times can be used for forensic odontology studies using the described methodology.http://www.sciencedirect.com/science/article/pii/S2212426814000384MitochondriaDNAForensic odontologyIsolation protocol |
| spellingShingle | Rahul Pandey Divya Mehrotra Pradnya Kowtal Abbas Ali Mahdi Rajiv Sarin Mitochondrial DNA from archived tissue samples kept in formalin for forensic odontology studies Journal of Oral Biology and Craniofacial Research Mitochondria DNA Forensic odontology Isolation protocol |
| title | Mitochondrial DNA from archived tissue samples kept in formalin for forensic odontology studies |
| title_full | Mitochondrial DNA from archived tissue samples kept in formalin for forensic odontology studies |
| title_fullStr | Mitochondrial DNA from archived tissue samples kept in formalin for forensic odontology studies |
| title_full_unstemmed | Mitochondrial DNA from archived tissue samples kept in formalin for forensic odontology studies |
| title_short | Mitochondrial DNA from archived tissue samples kept in formalin for forensic odontology studies |
| title_sort | mitochondrial dna from archived tissue samples kept in formalin for forensic odontology studies |
| topic | Mitochondria DNA Forensic odontology Isolation protocol |
| url | http://www.sciencedirect.com/science/article/pii/S2212426814000384 |
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