STUDY THE PATHOLOGICAL EFFECTS OF Klebsiella pneumoniae ISOLATED FROM LUNGS OF PNEUMONIA'S INFECTED SHEEP AND EXPERIMENTALLY INFECTED IN MICE

Currents study was designed to investigations about Klebsiella pneumoniaeinfection in sheep then evaluation its effects in mouse by experimentally infectionField study conducting on examination of one hundred100 sheep's lungs in KerbalaProvince, these lungs were send to laboratory of microbes f...

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Main Authors: Muna Sachit Hashim, Zahra Saleh Mahdi, Taghreed Jabbar Humadi, Eman Hashim Yousif, Thikra Abdulla Mahmood
Format: Article
Language:English
Published: College of Veterinary Medicine, University of Basrah, Iraq 2018-11-01
Series:Basrah Journal of Veterinary Research
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Online Access:https://bjvr.uobasrah.edu.iq/article_173000_2d78f2357d8cfc91556ce490b41e2840.pdf
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author Muna Sachit Hashim
Zahra Saleh Mahdi
Taghreed Jabbar Humadi
Eman Hashim Yousif
Thikra Abdulla Mahmood
author_facet Muna Sachit Hashim
Zahra Saleh Mahdi
Taghreed Jabbar Humadi
Eman Hashim Yousif
Thikra Abdulla Mahmood
author_sort Muna Sachit Hashim
collection DOAJ
description Currents study was designed to investigations about Klebsiella pneumoniaeinfection in sheep then evaluation its effects in mouse by experimentally infectionField study conducting on examination of one hundred100 sheep's lungs in KerbalaProvince, these lungs were send to laboratory of microbes for microbial examine.Results showed 44 % of lungs samples were infected with Klebsiella pneumoniae;18% of samples were negative for bacterial examination and the others 38 sampleswere infected with different type of bacteria but not frequently occurs as Klebsiella eLaboratory study, the isolated Klebsiella pneumoniae used to induce experimentalinfection in mice. Thirty 30 mixed mice at age of (4-6) weeks were used andsubdivided in to three 3 group; 1st contains 10 ten males injected with 0.25ml.IP. (1.5*10) suspension of bacteria; 2nd contains 10 ten females injected with 0.25ml.IP. (1.5*10) suspension of bacteria; 3rd contains mixed ten mice injected with Distilled Water.Scarified 4 four animals were done at the end of one week's until last 4 four week.Samples of tissues (liver, Kidney, lung, spleen, testes, uterus) were taken from eachmouse. Maine pathological lesions in lung of sheep infected with Klebsiellapneumoniae were suppurative broncho pneumonia, giant cell infiltration andgranulomatous foci. Tissues samples of experimentally infected mice showed generalabscesses at 1-2 weeks of infection, necrosis with granuloma and giant cell infiltrationat 3-4 weeks of infection. Conclusion: Klebsiella pneumoniae infects lung sheep and cause chronic suppurative pneumonia due to presence of giant cell as well asexperimentally infects mice and causes general suppurative and granulomatouslesions
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issn 1813-8497
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publisher College of Veterinary Medicine, University of Basrah, Iraq
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spelling doaj-art-bf9e2babe5144774896963565a06be932025-08-20T03:09:55ZengCollege of Veterinary Medicine, University of Basrah, IraqBasrah Journal of Veterinary Research1813-84972410-84562018-11-01173 (Proceeding of 6th International Scientific Conference, College of Veterinary Medicine University of Basrah, Iraq)37038010.23975/bjvetr.2018.173000173000STUDY THE PATHOLOGICAL EFFECTS OF Klebsiella pneumoniae ISOLATED FROM LUNGS OF PNEUMONIA'S INFECTED SHEEP AND EXPERIMENTALLY INFECTED IN MICEMuna Sachit Hashim0Zahra Saleh Mahdi1Taghreed Jabbar Humadi2Eman Hashim Yousif3Thikra Abdulla Mahmood4College of Veterinary Medicine. University of Baghdad,Baghdad,Iraq*College of Veterinary Medicine. University of Baghdad,Baghdad,Iraq*College of Veterinary Medicine. University of Baghdad,Baghdad,Iraq*College of Veterinary Medicine. University of Baghdad,Baghdad,IraqCollege of Medicine University of Kufa ,kufa,IraqCurrents study was designed to investigations about Klebsiella pneumoniaeinfection in sheep then evaluation its effects in mouse by experimentally infectionField study conducting on examination of one hundred100 sheep's lungs in KerbalaProvince, these lungs were send to laboratory of microbes for microbial examine.Results showed 44 % of lungs samples were infected with Klebsiella pneumoniae;18% of samples were negative for bacterial examination and the others 38 sampleswere infected with different type of bacteria but not frequently occurs as Klebsiella eLaboratory study, the isolated Klebsiella pneumoniae used to induce experimentalinfection in mice. Thirty 30 mixed mice at age of (4-6) weeks were used andsubdivided in to three 3 group; 1st contains 10 ten males injected with 0.25ml.IP. (1.5*10) suspension of bacteria; 2nd contains 10 ten females injected with 0.25ml.IP. (1.5*10) suspension of bacteria; 3rd contains mixed ten mice injected with Distilled Water.Scarified 4 four animals were done at the end of one week's until last 4 four week.Samples of tissues (liver, Kidney, lung, spleen, testes, uterus) were taken from eachmouse. Maine pathological lesions in lung of sheep infected with Klebsiellapneumoniae were suppurative broncho pneumonia, giant cell infiltration andgranulomatous foci. Tissues samples of experimentally infected mice showed generalabscesses at 1-2 weeks of infection, necrosis with granuloma and giant cell infiltrationat 3-4 weeks of infection. Conclusion: Klebsiella pneumoniae infects lung sheep and cause chronic suppurative pneumonia due to presence of giant cell as well asexperimentally infects mice and causes general suppurative and granulomatouslesionshttps://bjvr.uobasrah.edu.iq/article_173000_2d78f2357d8cfc91556ce490b41e2840.pdfbacterialpneumoniafield animalsmouse
spellingShingle Muna Sachit Hashim
Zahra Saleh Mahdi
Taghreed Jabbar Humadi
Eman Hashim Yousif
Thikra Abdulla Mahmood
STUDY THE PATHOLOGICAL EFFECTS OF Klebsiella pneumoniae ISOLATED FROM LUNGS OF PNEUMONIA'S INFECTED SHEEP AND EXPERIMENTALLY INFECTED IN MICE
Basrah Journal of Veterinary Research
bacterial
pneumonia
field animals
mouse
title STUDY THE PATHOLOGICAL EFFECTS OF Klebsiella pneumoniae ISOLATED FROM LUNGS OF PNEUMONIA'S INFECTED SHEEP AND EXPERIMENTALLY INFECTED IN MICE
title_full STUDY THE PATHOLOGICAL EFFECTS OF Klebsiella pneumoniae ISOLATED FROM LUNGS OF PNEUMONIA'S INFECTED SHEEP AND EXPERIMENTALLY INFECTED IN MICE
title_fullStr STUDY THE PATHOLOGICAL EFFECTS OF Klebsiella pneumoniae ISOLATED FROM LUNGS OF PNEUMONIA'S INFECTED SHEEP AND EXPERIMENTALLY INFECTED IN MICE
title_full_unstemmed STUDY THE PATHOLOGICAL EFFECTS OF Klebsiella pneumoniae ISOLATED FROM LUNGS OF PNEUMONIA'S INFECTED SHEEP AND EXPERIMENTALLY INFECTED IN MICE
title_short STUDY THE PATHOLOGICAL EFFECTS OF Klebsiella pneumoniae ISOLATED FROM LUNGS OF PNEUMONIA'S INFECTED SHEEP AND EXPERIMENTALLY INFECTED IN MICE
title_sort study the pathological effects of klebsiella pneumoniae isolated from lungs of pneumonia s infected sheep and experimentally infected in mice
topic bacterial
pneumonia
field animals
mouse
url https://bjvr.uobasrah.edu.iq/article_173000_2d78f2357d8cfc91556ce490b41e2840.pdf
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