Elucidation of fruit cracking mechanism in bael [Aegle marmelos (L.) Correa.] using physico-biochemical and de novo transcriptomic approaches

Fruit cracking in Bael [Aegle marmelos (L) Correa.] is a major physiological disorder which is influenced by factors like water stress, nutrient deficiency, and environmental conditions. This study aimed to identify key biochemical constituents, genes, and pathways affecting fruit cracking using phy...

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Main Authors: Vasanth Vinayak Vara Prasad, V.B. Patel, M.K. Dhakar, Bikash Das, Sujit Kumar Bishi, V.P. Bhadana, G.P. Mishra, Vishal Mhetre, S.K. Singh, Vishal Nath, Ram Asrey, Devendra Pandey
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:Plant Stress
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Online Access:http://www.sciencedirect.com/science/article/pii/S2667064X25000843
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Summary:Fruit cracking in Bael [Aegle marmelos (L) Correa.] is a major physiological disorder which is influenced by factors like water stress, nutrient deficiency, and environmental conditions. This study aimed to identify key biochemical constituents, genes, and pathways affecting fruit cracking using physical, biochemical, and transcriptomic analyses. Bael genotypes were categorized into three groups based on cracking incidence as tolerant (0 % cracking), moderately tolerant (>0–30 % cracking), and susceptible (>30 % cracking). Three genotypes from each category were selected for further analysis. Biochemical profiling revealed that total flavonoids, antioxidants, vanillic acid and soluble carbohydrates were predominant in the cracking-susceptible genotypes, while calcium and boron levels were significantly lower in these genotypes. Transcriptomic analysis using susceptible (Pant Aparna) and tolerant genotypes (ICAR-RCER 8–5) identified differentially expressed genes (DEGs) associated with cell wall and polysaccharide metabolism, phenolics and flavonoid biosynthesis, plant hormone biosynthesis and signaling, nutrient transport. Interestingly, aquaporin-encoding genes were found significantly upregulated in the cracking stage, while genes involved in MAPK signaling showed higher expression in the susceptible genotype. These transcriptomic changes were corroborated by biochemical findings, reinforcing their role in bael fruit cracking susceptibility. The insights gained from this study provide a foundation for developing cracking-tolerant bael cultivars and formulating management strategies to mitigate fruit cracking in bael.
ISSN:2667-064X