Preparation and evaluation of Brucella T4SS recombinant proteins in serodiagnosis of human brucellosis based on TMT-based proteomics technology
IntroductionBrucellosis, a significant zoonotic infectious disease, poses a global health threat. Accurate and efficient diagnosis is crucial for prevention, control, and treatment of brucellosis. VirB proteins, components of the Type IV secretion system (T4SS) in Brucella, play a pivotal role in ba...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2024.1514046/full |
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| author | Qi Wu Chen Sun Liping Guo Yujia Xie Jinpeng Zhang Jinpeng Zhang Dehui Yin Dehui Yin |
| author_facet | Qi Wu Chen Sun Liping Guo Yujia Xie Jinpeng Zhang Jinpeng Zhang Dehui Yin Dehui Yin |
| author_sort | Qi Wu |
| collection | DOAJ |
| description | IntroductionBrucellosis, a significant zoonotic infectious disease, poses a global health threat. Accurate and efficient diagnosis is crucial for prevention, control, and treatment of brucellosis. VirB proteins, components of the Type IV secretion system (T4SS) in Brucella, play a pivotal role in bacterial virulence and pathogenesis but have been understudied for their diagnostic potential.MethodsTandem Mass Tag (TMT) proteomics technology was utilized to identify highly expressed VirB proteins from wild-type Brucella strains. Recombinant T4SS proteins were prepared, and an indirect ELISA method was established for serological diagnosis of human brucellosis.ResultsSeven T4SS proteins (rVirB3, rVirB4, rVirB9, rBMEII0036, rVirB8, rVirB11, and rVirB10) were expressed used to construct the indirect ELISA method which showed high diagnostic accuracy. Sensitivity and specificity of the proteins exceeded 0.9100 and 0.9167, respectively, demonstrating good performance comparable to traditional LPS and Rose Bengal Ag antigens. Cross-reactivity was observed in a limited number of serum samples from febrile patients without brucellosis.ConclusionsThe study highlights the potential of VirB proteins as novel diagnostic antigens for human brucellosis. Future research can further optimize the use of VirB proteins in diagnostic assays and explore their applications in vaccine development. |
| format | Article |
| id | doaj-art-bf91336ac27b493cb69734dfef95b147 |
| institution | Kabale University |
| issn | 2235-2988 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj-art-bf91336ac27b493cb69734dfef95b1472025-01-16T06:10:17ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882025-01-011410.3389/fcimb.2024.15140461514046Preparation and evaluation of Brucella T4SS recombinant proteins in serodiagnosis of human brucellosis based on TMT-based proteomics technologyQi Wu0Chen Sun1Liping Guo2Yujia Xie3Jinpeng Zhang4Jinpeng Zhang5Dehui Yin6Dehui Yin7Jiangsu Engineering Research Center of Biological Data Mining and Healthcare Transformation, Xuzhou Medical University, Xuzhou, Jiangsu, ChinaDepartment of Clinical Laboratory, Huai’an Hospital of Huai’an City, Huai’an, ChinaJiangsu Engineering Research Center of Biological Data Mining and Healthcare Transformation, Xuzhou Medical University, Xuzhou, Jiangsu, ChinaJiangsu Engineering Research Center of Biological Data Mining and Healthcare Transformation, Xuzhou Medical University, Xuzhou, Jiangsu, ChinaJiangsu Engineering Research Center of Biological Data Mining and Healthcare Transformation, Xuzhou Medical University, Xuzhou, Jiangsu, ChinaKey Laboratory of Human Genetics and Environmental Medicine, Xuzhou Medical University, Xuzhou, ChinaJiangsu Engineering Research Center of Biological Data Mining and Healthcare Transformation, Xuzhou Medical University, Xuzhou, Jiangsu, ChinaKey Laboratory of Human Genetics and Environmental Medicine, Xuzhou Medical University, Xuzhou, ChinaIntroductionBrucellosis, a significant zoonotic infectious disease, poses a global health threat. Accurate and efficient diagnosis is crucial for prevention, control, and treatment of brucellosis. VirB proteins, components of the Type IV secretion system (T4SS) in Brucella, play a pivotal role in bacterial virulence and pathogenesis but have been understudied for their diagnostic potential.MethodsTandem Mass Tag (TMT) proteomics technology was utilized to identify highly expressed VirB proteins from wild-type Brucella strains. Recombinant T4SS proteins were prepared, and an indirect ELISA method was established for serological diagnosis of human brucellosis.ResultsSeven T4SS proteins (rVirB3, rVirB4, rVirB9, rBMEII0036, rVirB8, rVirB11, and rVirB10) were expressed used to construct the indirect ELISA method which showed high diagnostic accuracy. Sensitivity and specificity of the proteins exceeded 0.9100 and 0.9167, respectively, demonstrating good performance comparable to traditional LPS and Rose Bengal Ag antigens. Cross-reactivity was observed in a limited number of serum samples from febrile patients without brucellosis.ConclusionsThe study highlights the potential of VirB proteins as novel diagnostic antigens for human brucellosis. Future research can further optimize the use of VirB proteins in diagnostic assays and explore their applications in vaccine development.https://www.frontiersin.org/articles/10.3389/fcimb.2024.1514046/fullbrucellosistype IV secretion systemVirB proteinsTMT proteomicsserodiagnosis |
| spellingShingle | Qi Wu Chen Sun Liping Guo Yujia Xie Jinpeng Zhang Jinpeng Zhang Dehui Yin Dehui Yin Preparation and evaluation of Brucella T4SS recombinant proteins in serodiagnosis of human brucellosis based on TMT-based proteomics technology Frontiers in Cellular and Infection Microbiology brucellosis type IV secretion system VirB proteins TMT proteomics serodiagnosis |
| title | Preparation and evaluation of Brucella T4SS recombinant proteins in serodiagnosis of human brucellosis based on TMT-based proteomics technology |
| title_full | Preparation and evaluation of Brucella T4SS recombinant proteins in serodiagnosis of human brucellosis based on TMT-based proteomics technology |
| title_fullStr | Preparation and evaluation of Brucella T4SS recombinant proteins in serodiagnosis of human brucellosis based on TMT-based proteomics technology |
| title_full_unstemmed | Preparation and evaluation of Brucella T4SS recombinant proteins in serodiagnosis of human brucellosis based on TMT-based proteomics technology |
| title_short | Preparation and evaluation of Brucella T4SS recombinant proteins in serodiagnosis of human brucellosis based on TMT-based proteomics technology |
| title_sort | preparation and evaluation of brucella t4ss recombinant proteins in serodiagnosis of human brucellosis based on tmt based proteomics technology |
| topic | brucellosis type IV secretion system VirB proteins TMT proteomics serodiagnosis |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2024.1514046/full |
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