Cellular and synaptic properties of molecularly defined neurons in the external globus pallidus
Summary: The external globus pallidus (GPe) is a central basal ganglia (BG) nucleus, involved in various sensorimotor functions. The two main types of GPe neurons, the prototypic and arkypallidal neurons, differ in developmental origin, axonal projections, and functional roles. Using ex vivo whole-c...
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| Language: | English |
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Elsevier
2025-08-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004225012635 |
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| author | Linda M.C. Koene Wilhelm Thunberg Sten Grillner Gilad Silberberg Maya Ketzef |
| author_facet | Linda M.C. Koene Wilhelm Thunberg Sten Grillner Gilad Silberberg Maya Ketzef |
| author_sort | Linda M.C. Koene |
| collection | DOAJ |
| description | Summary: The external globus pallidus (GPe) is a central basal ganglia (BG) nucleus, involved in various sensorimotor functions. The two main types of GPe neurons, the prototypic and arkypallidal neurons, differ in developmental origin, axonal projections, and functional roles. Using ex vivo whole-cell patch-clamp recordings, we characterized the membrane properties, morphology, and synaptic connectivity of molecularly identified GPe neurons. Arkypallidal neurons had broader, slower action potentials with higher thresholds, and smaller somata, dendritic length, and surface area than prototypic neurons. Optogenetic activation of prototypic neurons inhibited both GPe subpopulations, but paired recordings revealed very sparse direct synaptic connectivity only between prototypic neurons. Moreover, parvalbumin-expressing prototypic neurons were not electrically coupled. This work provides a comprehensive description of the GPe microcircuitry and may provide insight into the functional role of the GPe within the BG network. |
| format | Article |
| id | doaj-art-bf8c49375b5f4b27a3d1775fa55328c0 |
| institution | DOAJ |
| issn | 2589-0042 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj-art-bf8c49375b5f4b27a3d1775fa55328c02025-08-20T03:12:49ZengElsevieriScience2589-00422025-08-0128811300210.1016/j.isci.2025.113002Cellular and synaptic properties of molecularly defined neurons in the external globus pallidusLinda M.C. Koene0Wilhelm Thunberg1Sten Grillner2Gilad Silberberg3Maya Ketzef4Department of Neuroscience, Karolinska Institutet, 171 77 Stockholm, SwedenDepartment of Neuroscience, Karolinska Institutet, 171 77 Stockholm, SwedenDepartment of Neuroscience, Karolinska Institutet, 171 77 Stockholm, SwedenDepartment of Neuroscience, Karolinska Institutet, 171 77 Stockholm, SwedenDepartment of Neuroscience, Karolinska Institutet, 171 77 Stockholm, Sweden; Corresponding authorSummary: The external globus pallidus (GPe) is a central basal ganglia (BG) nucleus, involved in various sensorimotor functions. The two main types of GPe neurons, the prototypic and arkypallidal neurons, differ in developmental origin, axonal projections, and functional roles. Using ex vivo whole-cell patch-clamp recordings, we characterized the membrane properties, morphology, and synaptic connectivity of molecularly identified GPe neurons. Arkypallidal neurons had broader, slower action potentials with higher thresholds, and smaller somata, dendritic length, and surface area than prototypic neurons. Optogenetic activation of prototypic neurons inhibited both GPe subpopulations, but paired recordings revealed very sparse direct synaptic connectivity only between prototypic neurons. Moreover, parvalbumin-expressing prototypic neurons were not electrically coupled. This work provides a comprehensive description of the GPe microcircuitry and may provide insight into the functional role of the GPe within the BG network.http://www.sciencedirect.com/science/article/pii/S2589004225012635PhysiologyNeuroscienceCellular neuroscienceCell biology |
| spellingShingle | Linda M.C. Koene Wilhelm Thunberg Sten Grillner Gilad Silberberg Maya Ketzef Cellular and synaptic properties of molecularly defined neurons in the external globus pallidus iScience Physiology Neuroscience Cellular neuroscience Cell biology |
| title | Cellular and synaptic properties of molecularly defined neurons in the external globus pallidus |
| title_full | Cellular and synaptic properties of molecularly defined neurons in the external globus pallidus |
| title_fullStr | Cellular and synaptic properties of molecularly defined neurons in the external globus pallidus |
| title_full_unstemmed | Cellular and synaptic properties of molecularly defined neurons in the external globus pallidus |
| title_short | Cellular and synaptic properties of molecularly defined neurons in the external globus pallidus |
| title_sort | cellular and synaptic properties of molecularly defined neurons in the external globus pallidus |
| topic | Physiology Neuroscience Cellular neuroscience Cell biology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004225012635 |
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