MicroRNA-137-3p Improves Nonalcoholic Fatty Liver Disease through Activating AMPKα
Nonalcoholic fatty liver disease (NAFLD) is one of the most common chronic liver diseases worldwide and can develop to nonalcoholic steatohepatitis and later hepatic cirrhosis with a high prevalence to hepatocellular carcinoma. Oxidative stress and chronic hepatic inflammation are implicated in the...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2021/4853355 |
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| author | Yuanjie Yu Chunping He Shiyun Tan Mengjun Huang Yitian Guo Ming Li Qian Zhang |
| author_facet | Yuanjie Yu Chunping He Shiyun Tan Mengjun Huang Yitian Guo Ming Li Qian Zhang |
| author_sort | Yuanjie Yu |
| collection | DOAJ |
| description | Nonalcoholic fatty liver disease (NAFLD) is one of the most common chronic liver diseases worldwide and can develop to nonalcoholic steatohepatitis and later hepatic cirrhosis with a high prevalence to hepatocellular carcinoma. Oxidative stress and chronic hepatic inflammation are implicated in the pathogenesis of NAFLD. MicroRNA-137-3p (miR-137-3p) are associated with oxidative stress and inflammation; however, its role and mechanism in NAFLD remain unclear. Mice were fed with a high-fat diet (HFD) for 24 weeks to establish the NAFLD model. To overexpress or suppress hepatic miR-137-3p expression, mice were intraperitoneally injected with the agomir, antagomir, or respective controls of miR-137-3p at a dose of 100 mg/kg weekly for 6 consecutive weeks before the mice were sacrificed. To validate the involvement of AMP-activated protein kinase alpha (AMPKα) or cAMP-specific phosphodiesterase 4D (PDE4D), HFD mice were intraperitoneally injected with 20 mg/kg compound C or 0.5 mg/kg rolipram every other day for 8 consecutive weeks before the mice were sacrificed. Hepatic miR-137-3p expression was significantly decreased in mice upon HFD stimulation. miR-137-3p agomir alleviated, while miR-137-3p antagomir facilitated HFD-induced oxidative stress, inflammation, and hepatic dysfunction in mice. Mechanistically, we revealed that miR-137-3p is directly bound to the 3′-untranslated region of PDE4D and subsequently increased hepatic cAMP level and protein kinase A activity, thereby activating the downstream AMPKα pathway. In summary, miR-137-3p improves NAFLD through activating AMPKα and it is a promising therapeutic candidate to treat NAFLD. |
| format | Article |
| id | doaj-art-bf79ddf6c4134520a0dcbd2363b60993 |
| institution | DOAJ |
| issn | 2210-7185 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-bf79ddf6c4134520a0dcbd2363b609932025-08-20T03:23:08ZengWileyAnalytical Cellular Pathology2210-71852021-01-01202110.1155/2021/4853355MicroRNA-137-3p Improves Nonalcoholic Fatty Liver Disease through Activating AMPKαYuanjie Yu0Chunping He1Shiyun Tan2Mengjun Huang3Yitian Guo4Ming Li5Qian Zhang6Department of GastroenterologyDepartment of GastroenterologyDepartment of GastroenterologyDepartment of NutritionDepartment of GastroenterologyDepartment of GastroenterologyDepartment of Infectious DiseasesNonalcoholic fatty liver disease (NAFLD) is one of the most common chronic liver diseases worldwide and can develop to nonalcoholic steatohepatitis and later hepatic cirrhosis with a high prevalence to hepatocellular carcinoma. Oxidative stress and chronic hepatic inflammation are implicated in the pathogenesis of NAFLD. MicroRNA-137-3p (miR-137-3p) are associated with oxidative stress and inflammation; however, its role and mechanism in NAFLD remain unclear. Mice were fed with a high-fat diet (HFD) for 24 weeks to establish the NAFLD model. To overexpress or suppress hepatic miR-137-3p expression, mice were intraperitoneally injected with the agomir, antagomir, or respective controls of miR-137-3p at a dose of 100 mg/kg weekly for 6 consecutive weeks before the mice were sacrificed. To validate the involvement of AMP-activated protein kinase alpha (AMPKα) or cAMP-specific phosphodiesterase 4D (PDE4D), HFD mice were intraperitoneally injected with 20 mg/kg compound C or 0.5 mg/kg rolipram every other day for 8 consecutive weeks before the mice were sacrificed. Hepatic miR-137-3p expression was significantly decreased in mice upon HFD stimulation. miR-137-3p agomir alleviated, while miR-137-3p antagomir facilitated HFD-induced oxidative stress, inflammation, and hepatic dysfunction in mice. Mechanistically, we revealed that miR-137-3p is directly bound to the 3′-untranslated region of PDE4D and subsequently increased hepatic cAMP level and protein kinase A activity, thereby activating the downstream AMPKα pathway. In summary, miR-137-3p improves NAFLD through activating AMPKα and it is a promising therapeutic candidate to treat NAFLD.http://dx.doi.org/10.1155/2021/4853355 |
| spellingShingle | Yuanjie Yu Chunping He Shiyun Tan Mengjun Huang Yitian Guo Ming Li Qian Zhang MicroRNA-137-3p Improves Nonalcoholic Fatty Liver Disease through Activating AMPKα Analytical Cellular Pathology |
| title | MicroRNA-137-3p Improves Nonalcoholic Fatty Liver Disease through Activating AMPKα |
| title_full | MicroRNA-137-3p Improves Nonalcoholic Fatty Liver Disease through Activating AMPKα |
| title_fullStr | MicroRNA-137-3p Improves Nonalcoholic Fatty Liver Disease through Activating AMPKα |
| title_full_unstemmed | MicroRNA-137-3p Improves Nonalcoholic Fatty Liver Disease through Activating AMPKα |
| title_short | MicroRNA-137-3p Improves Nonalcoholic Fatty Liver Disease through Activating AMPKα |
| title_sort | microrna 137 3p improves nonalcoholic fatty liver disease through activating ampkα |
| url | http://dx.doi.org/10.1155/2021/4853355 |
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