Investigation of the acetic acid stress response in Saccharomyces cerevisiae with mutated H3 residues
Enhanced levels of acetic acid reduce the activity of yeast strains employed for industrial fermentation-based applications. Therefore, unraveling the genetic factors underlying the regulation of the tolerance and sensitivity of yeast towards acetic acid is imperative for optimising various industri...
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| Language: | English |
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Shared Science Publishers OG
2023-08-01
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| Series: | Microbial Cell |
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| Online Access: | http://microbialcell.com/researcharticles/2023a-saha-microbial-cell/ |
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| author | Nitu Saha Swati Swagatika Raghuvir Singh Tomar |
| author_facet | Nitu Saha Swati Swagatika Raghuvir Singh Tomar |
| author_sort | Nitu Saha |
| collection | DOAJ |
| description | Enhanced levels of acetic acid reduce the activity of yeast strains employed for industrial fermentation-based applications. Therefore, unraveling the genetic factors underlying the regulation of the tolerance and sensitivity of yeast towards acetic acid is imperative for optimising various industrial processes. In this communication, we have attempted to decipher the acetic acid stress response of the previously reported acetic acid-sensitive histone mutants. Revalidation using spot-test assays and growth curves revealed that five of these mutants, viz., H3K18Q, H3S28A, H3K42Q, H3Q68A, and H3F104A, are most sensitive towards the tested acetic acid concentrations. These mutants demonstrated enhanced acetic acid stress response as evidenced by the increased expression levels of AIF1, reactive oxygen species (ROS) generation, chromatin fragmentation, and aggregated actin cytoskeleton. Additionally, the mutants exhibited active cell wall damage response upon acetic acid treatment, as demonstrated by increased Slt2-phosphorylation and expression of cell wall integrity genes. Interestingly, the mutants demonstrated increased sensitivity to cell wall stress-causing agents. Finally, screening of histone H3 N-terminal tail truncation mutants revealed that the tail truncations exhibit general sensitivity to acetic acid stress. Some of these N-terminal tail truncation mutants viz., H3 [del 1-24], H3 [del 1-28], H3 [del 9-24], and H3 [del 25-36] are also sensitive to cell wall stress agents such as Congo red and caffeine suggesting that their enhanced acetic acid sensitivity may be due to cell wall stress induced by acetic acid. |
| format | Article |
| id | doaj-art-bf73eb38a3e945b29d30f75a4a31a2b0 |
| institution | OA Journals |
| issn | 2311-2638 |
| language | English |
| publishDate | 2023-08-01 |
| publisher | Shared Science Publishers OG |
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| series | Microbial Cell |
| spelling | doaj-art-bf73eb38a3e945b29d30f75a4a31a2b02025-08-20T02:04:34ZengShared Science Publishers OGMicrobial Cell2311-26382023-08-01101021723210.15698/mic2023.10.806Investigation of the acetic acid stress response in Saccharomyces cerevisiae with mutated H3 residuesNitu Saha0Swati Swagatika1Raghuvir Singh Tomar2Laboratory of Chromatin Biology, Department of Biological Sciences, Indian Institute of Science Education and Research Bhopal, 462066, Madhya Pradesh, India.Laboratory of Chromatin Biology, Department of Biological Sciences, Indian Institute of Science Education and Research Bhopal, 462066, Madhya Pradesh, India.Laboratory of Chromatin Biology, Department of Biological Sciences, Indian Institute of Science Education and Research Bhopal, 462066, Madhya Pradesh, India.Enhanced levels of acetic acid reduce the activity of yeast strains employed for industrial fermentation-based applications. Therefore, unraveling the genetic factors underlying the regulation of the tolerance and sensitivity of yeast towards acetic acid is imperative for optimising various industrial processes. In this communication, we have attempted to decipher the acetic acid stress response of the previously reported acetic acid-sensitive histone mutants. Revalidation using spot-test assays and growth curves revealed that five of these mutants, viz., H3K18Q, H3S28A, H3K42Q, H3Q68A, and H3F104A, are most sensitive towards the tested acetic acid concentrations. These mutants demonstrated enhanced acetic acid stress response as evidenced by the increased expression levels of AIF1, reactive oxygen species (ROS) generation, chromatin fragmentation, and aggregated actin cytoskeleton. Additionally, the mutants exhibited active cell wall damage response upon acetic acid treatment, as demonstrated by increased Slt2-phosphorylation and expression of cell wall integrity genes. Interestingly, the mutants demonstrated increased sensitivity to cell wall stress-causing agents. Finally, screening of histone H3 N-terminal tail truncation mutants revealed that the tail truncations exhibit general sensitivity to acetic acid stress. Some of these N-terminal tail truncation mutants viz., H3 [del 1-24], H3 [del 1-28], H3 [del 9-24], and H3 [del 25-36] are also sensitive to cell wall stress agents such as Congo red and caffeine suggesting that their enhanced acetic acid sensitivity may be due to cell wall stress induced by acetic acid.http://microbialcell.com/researcharticles/2023a-saha-microbial-cell/acetic acidh3 point mutantsh3 n-terminal tail truncation mutantsaif1reactive oxygen species |
| spellingShingle | Nitu Saha Swati Swagatika Raghuvir Singh Tomar Investigation of the acetic acid stress response in Saccharomyces cerevisiae with mutated H3 residues Microbial Cell acetic acid h3 point mutants h3 n-terminal tail truncation mutants aif1 reactive oxygen species |
| title | Investigation of the acetic acid stress response in Saccharomyces cerevisiae with mutated H3 residues |
| title_full | Investigation of the acetic acid stress response in Saccharomyces cerevisiae with mutated H3 residues |
| title_fullStr | Investigation of the acetic acid stress response in Saccharomyces cerevisiae with mutated H3 residues |
| title_full_unstemmed | Investigation of the acetic acid stress response in Saccharomyces cerevisiae with mutated H3 residues |
| title_short | Investigation of the acetic acid stress response in Saccharomyces cerevisiae with mutated H3 residues |
| title_sort | investigation of the acetic acid stress response in saccharomyces cerevisiae with mutated h3 residues |
| topic | acetic acid h3 point mutants h3 n-terminal tail truncation mutants aif1 reactive oxygen species |
| url | http://microbialcell.com/researcharticles/2023a-saha-microbial-cell/ |
| work_keys_str_mv | AT nitusaha investigationoftheaceticacidstressresponseinsaccharomycescerevisiaewithmutatedh3residues AT swatiswagatika investigationoftheaceticacidstressresponseinsaccharomycescerevisiaewithmutatedh3residues AT raghuvirsinghtomar investigationoftheaceticacidstressresponseinsaccharomycescerevisiaewithmutatedh3residues |