Bergenin Attenuates Hepatic Fibrosis by Regulating Autophagy Mediated by the PPAR-γ/TGF-β Pathway
Liver fibrosis is a pathological process involving diffuse extracellular matrix (ECM) deposition in the liver. It is typical of many chronic liver diseases, including cirrhosis, and effective drugs are needed. In this study, we explored the protective effect of bergenin on liver fibrosis induced by...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-01-01
|
| Series: | PPAR Research |
| Online Access: | http://dx.doi.org/10.1155/2020/6694214 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850175496215592960 |
|---|---|
| author | Yujing Xia Jingjing Li Kan Chen Jiao Feng Chuanyong Guo |
| author_facet | Yujing Xia Jingjing Li Kan Chen Jiao Feng Chuanyong Guo |
| author_sort | Yujing Xia |
| collection | DOAJ |
| description | Liver fibrosis is a pathological process involving diffuse extracellular matrix (ECM) deposition in the liver. It is typical of many chronic liver diseases, including cirrhosis, and effective drugs are needed. In this study, we explored the protective effect of bergenin on liver fibrosis induced by carbon tetrachloride and bile duct ligation. A variety of molecular biological methods (qRT-PCR, western blotting, and immunohistochemistry) were employed to confirm the increased degree of hepatocyte injury and ECM formation in the disease model, consistent with autophagy and activation of the TGF-β pathway. Bergenin activated PPAR-γ and inhibited TGF-β and autophagy and decreased liver fibrosis by inhibiting hepatocyte necrosis and ECM formation in a dose-dependent manner. The results suggest that bergenin may be a promising drug candidate for the treatment of liver fibrosis. |
| format | Article |
| id | doaj-art-bf70c7efb70a40d485054de7e06c9aaa |
| institution | OA Journals |
| issn | 1687-4757 1687-4765 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | PPAR Research |
| spelling | doaj-art-bf70c7efb70a40d485054de7e06c9aaa2025-08-20T02:19:26ZengWileyPPAR Research1687-47571687-47652020-01-01202010.1155/2020/66942146694214Bergenin Attenuates Hepatic Fibrosis by Regulating Autophagy Mediated by the PPAR-γ/TGF-β PathwayYujing Xia0Jingjing Li1Kan Chen2Jiao Feng3Chuanyong Guo4Department of Gastroenterology, Shanghai Tenth People’s Hospital, School of Clinical Medicine of Nanjing Medical University, Shanghai 200072, ChinaDepartment of Gastroenterology, Shanghai Tenth People’s Hospital, School of Clinical Medicine of Nanjing Medical University, Shanghai 200072, ChinaDepartment of Gastroenterology, Shanghai Tenth People’s Hospital, School of Clinical Medicine of Nanjing Medical University, Shanghai 200072, ChinaDepartment of Gastroenterology, Shanghai Tenth People’s Hospital, School of Clinical Medicine of Nanjing Medical University, Shanghai 200072, ChinaDepartment of Gastroenterology, Shanghai Tenth People’s Hospital, School of Clinical Medicine of Nanjing Medical University, Shanghai 200072, ChinaLiver fibrosis is a pathological process involving diffuse extracellular matrix (ECM) deposition in the liver. It is typical of many chronic liver diseases, including cirrhosis, and effective drugs are needed. In this study, we explored the protective effect of bergenin on liver fibrosis induced by carbon tetrachloride and bile duct ligation. A variety of molecular biological methods (qRT-PCR, western blotting, and immunohistochemistry) were employed to confirm the increased degree of hepatocyte injury and ECM formation in the disease model, consistent with autophagy and activation of the TGF-β pathway. Bergenin activated PPAR-γ and inhibited TGF-β and autophagy and decreased liver fibrosis by inhibiting hepatocyte necrosis and ECM formation in a dose-dependent manner. The results suggest that bergenin may be a promising drug candidate for the treatment of liver fibrosis.http://dx.doi.org/10.1155/2020/6694214 |
| spellingShingle | Yujing Xia Jingjing Li Kan Chen Jiao Feng Chuanyong Guo Bergenin Attenuates Hepatic Fibrosis by Regulating Autophagy Mediated by the PPAR-γ/TGF-β Pathway PPAR Research |
| title | Bergenin Attenuates Hepatic Fibrosis by Regulating Autophagy Mediated by the PPAR-γ/TGF-β Pathway |
| title_full | Bergenin Attenuates Hepatic Fibrosis by Regulating Autophagy Mediated by the PPAR-γ/TGF-β Pathway |
| title_fullStr | Bergenin Attenuates Hepatic Fibrosis by Regulating Autophagy Mediated by the PPAR-γ/TGF-β Pathway |
| title_full_unstemmed | Bergenin Attenuates Hepatic Fibrosis by Regulating Autophagy Mediated by the PPAR-γ/TGF-β Pathway |
| title_short | Bergenin Attenuates Hepatic Fibrosis by Regulating Autophagy Mediated by the PPAR-γ/TGF-β Pathway |
| title_sort | bergenin attenuates hepatic fibrosis by regulating autophagy mediated by the ppar γ tgf β pathway |
| url | http://dx.doi.org/10.1155/2020/6694214 |
| work_keys_str_mv | AT yujingxia bergeninattenuateshepaticfibrosisbyregulatingautophagymediatedbytheppargtgfbpathway AT jingjingli bergeninattenuateshepaticfibrosisbyregulatingautophagymediatedbytheppargtgfbpathway AT kanchen bergeninattenuateshepaticfibrosisbyregulatingautophagymediatedbytheppargtgfbpathway AT jiaofeng bergeninattenuateshepaticfibrosisbyregulatingautophagymediatedbytheppargtgfbpathway AT chuanyongguo bergeninattenuateshepaticfibrosisbyregulatingautophagymediatedbytheppargtgfbpathway |