Acid hydrolysis and microwave digestion enhanced protein extraction from red seaweed Palmaria palmata
Palmaria palmata proteins have limited dietary use due to ineffective protein extraction methods. This study explores pretreatments, including homogenization, ultrasonication, microwave digestion, surfactant addition, acid hydrolysis, and viscozyme hydrolysis, before conventional alkaline solubiliza...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-01-01
|
| Series: | Food Chemistry: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157525000689 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1823856694499737600 |
|---|---|
| author | Anushi Madushani Wijethunga Quan Sophia He Balakrishnan Prithiviraj Xiaohong Sun |
| author_facet | Anushi Madushani Wijethunga Quan Sophia He Balakrishnan Prithiviraj Xiaohong Sun |
| author_sort | Anushi Madushani Wijethunga |
| collection | DOAJ |
| description | Palmaria palmata proteins have limited dietary use due to ineffective protein extraction methods. This study explores pretreatments, including homogenization, ultrasonication, microwave digestion, surfactant addition, acid hydrolysis, and viscozyme hydrolysis, before conventional alkaline solubilization and acidic precipitation to improve protein extraction yield and purity. The results showed that microwave digestion (500 W, 30 min) and acid hydrolysis (2 M H2SO4, 95 °C, 2 h) significantly increased final yield from 10.4 ± 0.4 % to 47.6 ± 3.9 % and 46.5 ± 3.4 %, respectively. Specifically, alkaline solubilization yields reached 99.7 ± 3.7 % (microwave) and 99.2 ± 3.2 % (acid hydrolysis) (p < 0.05). Protein purity reached 89.8 ± 1.1 % with microwave digestion and 65.3 ± 1.2 % with acid hydrolysis. The protein yield increment can be attributed to effective cell wall disruption, as evidenced by SEM imaging and CLSM imaging. SDS-PAGE showed that the proteins resulting from microwave and acid hydrolysis have molecular weights below 10 kDa. This study developed effective methods with potential for scaling up to enhance protein extraction from Palmaria palmata. |
| format | Article |
| id | doaj-art-bf6d509dc369458c903804f27c1ed768 |
| institution | Kabale University |
| issn | 2590-1575 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj-art-bf6d509dc369458c903804f27c1ed7682025-02-12T05:32:38ZengElsevierFood Chemistry: X2590-15752025-01-0125102222Acid hydrolysis and microwave digestion enhanced protein extraction from red seaweed Palmaria palmataAnushi Madushani Wijethunga0Quan Sophia He1Balakrishnan Prithiviraj2Xiaohong Sun3Department of Plant, Food and Environmental Sciences, Faculty of Agriculture, Dalhousie University, Truro, Nova Scotia B2N 5E3, CanadaDepartment of Engineering, Faculty of Agriculture, Dalhousie University, Truro, Nova Scotia B2N 5E3, CanadaDepartment of Plant, Food and Environmental Sciences, Faculty of Agriculture, Dalhousie University, Truro, Nova Scotia B2N 5E3, CanadaDepartment of Plant, Food and Environmental Sciences, Faculty of Agriculture, Dalhousie University, Truro, Nova Scotia B2N 5E3, Canada; Corresponding author.Palmaria palmata proteins have limited dietary use due to ineffective protein extraction methods. This study explores pretreatments, including homogenization, ultrasonication, microwave digestion, surfactant addition, acid hydrolysis, and viscozyme hydrolysis, before conventional alkaline solubilization and acidic precipitation to improve protein extraction yield and purity. The results showed that microwave digestion (500 W, 30 min) and acid hydrolysis (2 M H2SO4, 95 °C, 2 h) significantly increased final yield from 10.4 ± 0.4 % to 47.6 ± 3.9 % and 46.5 ± 3.4 %, respectively. Specifically, alkaline solubilization yields reached 99.7 ± 3.7 % (microwave) and 99.2 ± 3.2 % (acid hydrolysis) (p < 0.05). Protein purity reached 89.8 ± 1.1 % with microwave digestion and 65.3 ± 1.2 % with acid hydrolysis. The protein yield increment can be attributed to effective cell wall disruption, as evidenced by SEM imaging and CLSM imaging. SDS-PAGE showed that the proteins resulting from microwave and acid hydrolysis have molecular weights below 10 kDa. This study developed effective methods with potential for scaling up to enhance protein extraction from Palmaria palmata.http://www.sciencedirect.com/science/article/pii/S2590157525000689Palmaria palmataMicrowaveAcid hydrolysisProtein extraction yieldMicrostructureSustainable protein source |
| spellingShingle | Anushi Madushani Wijethunga Quan Sophia He Balakrishnan Prithiviraj Xiaohong Sun Acid hydrolysis and microwave digestion enhanced protein extraction from red seaweed Palmaria palmata Food Chemistry: X Palmaria palmata Microwave Acid hydrolysis Protein extraction yield Microstructure Sustainable protein source |
| title | Acid hydrolysis and microwave digestion enhanced protein extraction from red seaweed Palmaria palmata |
| title_full | Acid hydrolysis and microwave digestion enhanced protein extraction from red seaweed Palmaria palmata |
| title_fullStr | Acid hydrolysis and microwave digestion enhanced protein extraction from red seaweed Palmaria palmata |
| title_full_unstemmed | Acid hydrolysis and microwave digestion enhanced protein extraction from red seaweed Palmaria palmata |
| title_short | Acid hydrolysis and microwave digestion enhanced protein extraction from red seaweed Palmaria palmata |
| title_sort | acid hydrolysis and microwave digestion enhanced protein extraction from red seaweed palmaria palmata |
| topic | Palmaria palmata Microwave Acid hydrolysis Protein extraction yield Microstructure Sustainable protein source |
| url | http://www.sciencedirect.com/science/article/pii/S2590157525000689 |
| work_keys_str_mv | AT anushimadushaniwijethunga acidhydrolysisandmicrowavedigestionenhancedproteinextractionfromredseaweedpalmariapalmata AT quansophiahe acidhydrolysisandmicrowavedigestionenhancedproteinextractionfromredseaweedpalmariapalmata AT balakrishnanprithiviraj acidhydrolysisandmicrowavedigestionenhancedproteinextractionfromredseaweedpalmariapalmata AT xiaohongsun acidhydrolysisandmicrowavedigestionenhancedproteinextractionfromredseaweedpalmariapalmata |