Probiotic potential and safety properties of Limosilactobacillus fermentum A51 with high exopolysaccharide production
IntroductionExopolysaccharides (EPS) produced by Lactic acid bacteria have many health benefits and unique physicochemical properties. They are widely used in the food industry to improve viscosity, mouthfeel, and textural properties of foods. In our previous studies, Limosilactobacillus fermentum A...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-01-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1498352/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832592434085756928 |
|---|---|
| author | Guangqiang Wei Daodian Wang Daodian Wang Teng Wang Gao Wang Yunmei Chai Yufang Li Minhui Mei Hao Wang Aixiang Huang |
| author_facet | Guangqiang Wei Daodian Wang Daodian Wang Teng Wang Gao Wang Yunmei Chai Yufang Li Minhui Mei Hao Wang Aixiang Huang |
| author_sort | Guangqiang Wei |
| collection | DOAJ |
| description | IntroductionExopolysaccharides (EPS) produced by Lactic acid bacteria have many health benefits and unique physicochemical properties. They are widely used in the food industry to improve viscosity, mouthfeel, and textural properties of foods. In our previous studies, Limosilactobacillus fermentum A51 (L. fermentum A51) isolated from yak yogurt exhibited high EPS production capacity and was applied to improve the texture of yogurt. In this study, whole genome sequencing analysis and corresponding in vitro assays were performed to investigate the probiotic potential and safety properties of L. fermentum A51.ResultsScanning electron microscopy (SEM) observed that L. fermentum strain A51 adhered into clusters and its colony exhibited the obvious silk drawing phenomenon. Whole genome mapping revealed that L. fermentum A51 genome is 2,188,538 bp, and with an average guanine and cytosine (GC) content of 51.28%. PGAAP annotation identified 2,152 protein-encoding genes and 58 rRNAs, 15 tRNAs, and 5 5sRNAs. Hemolysis and antibiotic resistance tests, combined with the analysis of genes involved in antibiotic resistance, virulence factor, and hemolysins, suggested that L. fermentum A51 is safe. Fifty-one carbohydrate active enzyme genes in the whole genome sequence of L. fermentum A51 were annotated by carbohydrate active enzymes (CAZymes). Furthermore, L. fermentum A51 possesses adhesion, acid tolerance, bile salt tolerance, and heat tolerance genes (srtA, tuf, Bsh, nhaC, Ntn, cfa), antioxidant (nrfA, npr, nox2, tps), antibacterial genes (Idh and Dld) EPS synthesis-related genes (glf, epsG, gtf, Wzz, Wzx, Wzy), and signal molecule A1-2 synthesis-related genes (luxS, pfs). These probiotic genes were verified by quantitative real-time PCR. In vitro assays confirmed that L. fermentum A51 showed good tolerance to simulated gastrointestinal tract (8.49 log CFU/mL), 0.3% bile salt (39.06%), and possessed adhesion (86.92%), antioxidant (70.60–89.71%), and antimicrobial activities, as well as EPS and signaling molecule AI-2 synthesis capacities.ConclusionCollectively, our findings have confirmed that L. fermentum A51 is safe and exhibits good probiotic properties, thus recommending its potential application in the production of value-added fermented dairy products. |
| format | Article |
| id | doaj-art-bf6a7e97688a4a5c8064d8b08c3660ee |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-bf6a7e97688a4a5c8064d8b08c3660ee2025-01-21T08:37:03ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-01-011610.3389/fmicb.2025.14983521498352Probiotic potential and safety properties of Limosilactobacillus fermentum A51 with high exopolysaccharide productionGuangqiang Wei0Daodian Wang1Daodian Wang2Teng Wang3Gao Wang4Yunmei Chai5Yufang Li6Minhui Mei7Hao Wang8Aixiang Huang9College of Food Science and Technology, Yunnan Agricultural University, Kunming, ChinaCollege of Food Science and Technology, Yunnan Agricultural University, Kunming, ChinaSericulture and Apiculture Research Institute, Yunnan Academy of Agricultural Sciences, Mengzi, Yunnan, ChinaCollege of Food Science and Technology, Yunnan Agricultural University, Kunming, ChinaHeqing County Inspection and Testing Institute, Heqing, Yunnan, ChinaCollege of Food Science and Technology, Yunnan Agricultural University, Kunming, ChinaCollege of Food Science and Technology, Yunnan Agricultural University, Kunming, ChinaCollege of Food Science and Technology, Yunnan Agricultural University, Kunming, ChinaCollege of Food Science and Technology, Yunnan Agricultural University, Kunming, ChinaCollege of Food Science and Technology, Yunnan Agricultural University, Kunming, ChinaIntroductionExopolysaccharides (EPS) produced by Lactic acid bacteria have many health benefits and unique physicochemical properties. They are widely used in the food industry to improve viscosity, mouthfeel, and textural properties of foods. In our previous studies, Limosilactobacillus fermentum A51 (L. fermentum A51) isolated from yak yogurt exhibited high EPS production capacity and was applied to improve the texture of yogurt. In this study, whole genome sequencing analysis and corresponding in vitro assays were performed to investigate the probiotic potential and safety properties of L. fermentum A51.ResultsScanning electron microscopy (SEM) observed that L. fermentum strain A51 adhered into clusters and its colony exhibited the obvious silk drawing phenomenon. Whole genome mapping revealed that L. fermentum A51 genome is 2,188,538 bp, and with an average guanine and cytosine (GC) content of 51.28%. PGAAP annotation identified 2,152 protein-encoding genes and 58 rRNAs, 15 tRNAs, and 5 5sRNAs. Hemolysis and antibiotic resistance tests, combined with the analysis of genes involved in antibiotic resistance, virulence factor, and hemolysins, suggested that L. fermentum A51 is safe. Fifty-one carbohydrate active enzyme genes in the whole genome sequence of L. fermentum A51 were annotated by carbohydrate active enzymes (CAZymes). Furthermore, L. fermentum A51 possesses adhesion, acid tolerance, bile salt tolerance, and heat tolerance genes (srtA, tuf, Bsh, nhaC, Ntn, cfa), antioxidant (nrfA, npr, nox2, tps), antibacterial genes (Idh and Dld) EPS synthesis-related genes (glf, epsG, gtf, Wzz, Wzx, Wzy), and signal molecule A1-2 synthesis-related genes (luxS, pfs). These probiotic genes were verified by quantitative real-time PCR. In vitro assays confirmed that L. fermentum A51 showed good tolerance to simulated gastrointestinal tract (8.49 log CFU/mL), 0.3% bile salt (39.06%), and possessed adhesion (86.92%), antioxidant (70.60–89.71%), and antimicrobial activities, as well as EPS and signaling molecule AI-2 synthesis capacities.ConclusionCollectively, our findings have confirmed that L. fermentum A51 is safe and exhibits good probiotic properties, thus recommending its potential application in the production of value-added fermented dairy products.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1498352/fullLimosilactobacillusfermentum A51whole genome sequencingprobiotic propertiesphenotype analysissafety |
| spellingShingle | Guangqiang Wei Daodian Wang Daodian Wang Teng Wang Gao Wang Yunmei Chai Yufang Li Minhui Mei Hao Wang Aixiang Huang Probiotic potential and safety properties of Limosilactobacillus fermentum A51 with high exopolysaccharide production Frontiers in Microbiology Limosilactobacillusfermentum A51 whole genome sequencing probiotic properties phenotype analysis safety |
| title | Probiotic potential and safety properties of Limosilactobacillus fermentum A51 with high exopolysaccharide production |
| title_full | Probiotic potential and safety properties of Limosilactobacillus fermentum A51 with high exopolysaccharide production |
| title_fullStr | Probiotic potential and safety properties of Limosilactobacillus fermentum A51 with high exopolysaccharide production |
| title_full_unstemmed | Probiotic potential and safety properties of Limosilactobacillus fermentum A51 with high exopolysaccharide production |
| title_short | Probiotic potential and safety properties of Limosilactobacillus fermentum A51 with high exopolysaccharide production |
| title_sort | probiotic potential and safety properties of limosilactobacillus fermentum a51 with high exopolysaccharide production |
| topic | Limosilactobacillusfermentum A51 whole genome sequencing probiotic properties phenotype analysis safety |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1498352/full |
| work_keys_str_mv | AT guangqiangwei probioticpotentialandsafetypropertiesoflimosilactobacillusfermentuma51withhighexopolysaccharideproduction AT daodianwang probioticpotentialandsafetypropertiesoflimosilactobacillusfermentuma51withhighexopolysaccharideproduction AT daodianwang probioticpotentialandsafetypropertiesoflimosilactobacillusfermentuma51withhighexopolysaccharideproduction AT tengwang probioticpotentialandsafetypropertiesoflimosilactobacillusfermentuma51withhighexopolysaccharideproduction AT gaowang probioticpotentialandsafetypropertiesoflimosilactobacillusfermentuma51withhighexopolysaccharideproduction AT yunmeichai probioticpotentialandsafetypropertiesoflimosilactobacillusfermentuma51withhighexopolysaccharideproduction AT yufangli probioticpotentialandsafetypropertiesoflimosilactobacillusfermentuma51withhighexopolysaccharideproduction AT minhuimei probioticpotentialandsafetypropertiesoflimosilactobacillusfermentuma51withhighexopolysaccharideproduction AT haowang probioticpotentialandsafetypropertiesoflimosilactobacillusfermentuma51withhighexopolysaccharideproduction AT aixianghuang probioticpotentialandsafetypropertiesoflimosilactobacillusfermentuma51withhighexopolysaccharideproduction |