Isolation, screening and characterization of xylose-fermenting yeasts isolated from sawdust
Yeasts have been less frequently reported as xylanase producers compared to bacteria and filamentous fungi. Different cellulosic materials including sawdust are produced on a large scale and these can be used for the production of useful enzymes such as xylanases. Xylanases are a group of enzymes th...
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Visayas State University
2024-06-01
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| Series: | Annals of Tropical Research |
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| Online Access: | https://atr.vsu.edu.ph/article/view/10 |
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| author | Adelabu Blessing Michael Taiwo Soetan OO |
| author_facet | Adelabu Blessing Michael Taiwo Soetan OO |
| author_sort | Adelabu Blessing |
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| description | Yeasts have been less frequently reported as xylanase producers compared to bacteria and filamentous fungi. Different cellulosic materials including sawdust are produced on a large scale and these can be used for the production of useful enzymes such as xylanases. Xylanases are a group of enzymes that hydrolyze plant fibers made of xylan hemicellulose. Xylose-fermenting yeasts isolated from soil at a wood processing factory were isolated and qualitatively and quantitatively screened for xylanase production using xylose supplemented medium and congo red as indicator. Xylanase enzyme was produced using different xylose concentrations (0.5%, 1.0%, 1.5% and 2.0%). Pichia chambardii isolate, which was later identified as Wickerhamomyces chambardii by molecular techniques, showed the highest xylanase activity of 199.31U mL-1. Maximum xylanase activity (275.83U mL-1) was achieved at 1.5 %w/v xylose. This study showed that yeasts have a high potential for the production of xylanase enzymes. |
| format | Article |
| id | doaj-art-bf625affba5a4b6d8046504a8b63700f |
| institution | Kabale University |
| issn | 0116-0710 2704-3541 |
| language | English |
| publishDate | 2024-06-01 |
| publisher | Visayas State University |
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| series | Annals of Tropical Research |
| spelling | doaj-art-bf625affba5a4b6d8046504a8b63700f2025-01-10T02:07:46ZengVisayas State UniversityAnnals of Tropical Research0116-07102704-35412024-06-01461111https://doi.org/10.32945/atr4611.2024Isolation, screening and characterization of xylose-fermenting yeasts isolated from sawdustAdelabu Blessing0https://orcid.org/0000-0002-5469-2175Michael Taiwo 1https://orcid.org/0000-0001-9594-9355Soetan OO2Department of Microbiology, Chrisland University, Abeokuta, NigeriaDepartment of Microbiology, Chrisland University, Abeokuta, NigeriaDepartment of Microbiology, Chrisland University, Abeokuta, NigeriaYeasts have been less frequently reported as xylanase producers compared to bacteria and filamentous fungi. Different cellulosic materials including sawdust are produced on a large scale and these can be used for the production of useful enzymes such as xylanases. Xylanases are a group of enzymes that hydrolyze plant fibers made of xylan hemicellulose. Xylose-fermenting yeasts isolated from soil at a wood processing factory were isolated and qualitatively and quantitatively screened for xylanase production using xylose supplemented medium and congo red as indicator. Xylanase enzyme was produced using different xylose concentrations (0.5%, 1.0%, 1.5% and 2.0%). Pichia chambardii isolate, which was later identified as Wickerhamomyces chambardii by molecular techniques, showed the highest xylanase activity of 199.31U mL-1. Maximum xylanase activity (275.83U mL-1) was achieved at 1.5 %w/v xylose. This study showed that yeasts have a high potential for the production of xylanase enzymes.https://atr.vsu.edu.ph/article/view/10congo redlignocellulosicsawdustwikerhamomyces chambardiixylanase |
| spellingShingle | Adelabu Blessing Michael Taiwo Soetan OO Isolation, screening and characterization of xylose-fermenting yeasts isolated from sawdust Annals of Tropical Research congo red lignocellulosic sawdust wikerhamomyces chambardii xylanase |
| title | Isolation, screening and characterization of xylose-fermenting yeasts isolated from sawdust |
| title_full | Isolation, screening and characterization of xylose-fermenting yeasts isolated from sawdust |
| title_fullStr | Isolation, screening and characterization of xylose-fermenting yeasts isolated from sawdust |
| title_full_unstemmed | Isolation, screening and characterization of xylose-fermenting yeasts isolated from sawdust |
| title_short | Isolation, screening and characterization of xylose-fermenting yeasts isolated from sawdust |
| title_sort | isolation screening and characterization of xylose fermenting yeasts isolated from sawdust |
| topic | congo red lignocellulosic sawdust wikerhamomyces chambardii xylanase |
| url | https://atr.vsu.edu.ph/article/view/10 |
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