Protocol for decellularizing and delipidizing human pancreatic tissue into native hydrogel
Summary: We present a protocol for transforming human pancreatic tissue into a decellularized and delipidized extracellular matrix (ECM) for hydrogel/scaffold applications. We describe steps for decellularization and delipidization using sodium deoxycholate and sequential ethanol, acetone, and hexan...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-09-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725004034 |
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| _version_ | 1849420880023650304 |
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| author | Connie S. Chamberlain Daniel M. Tremmel Ayesha Palwasha Khan Ellen Abad Santos Deep Kapadia Caterra Leavens Katherine M. Gorski Rawan Hamadeh Austin K. Feeney Jon Odorico Sara D. Sackett |
| author_facet | Connie S. Chamberlain Daniel M. Tremmel Ayesha Palwasha Khan Ellen Abad Santos Deep Kapadia Caterra Leavens Katherine M. Gorski Rawan Hamadeh Austin K. Feeney Jon Odorico Sara D. Sackett |
| author_sort | Connie S. Chamberlain |
| collection | DOAJ |
| description | Summary: We present a protocol for transforming human pancreatic tissue into a decellularized and delipidized extracellular matrix (ECM) for hydrogel/scaffold applications. We describe steps for decellularization and delipidization using sodium deoxycholate and sequential ethanol, acetone, and hexane washes and removal of nucleic acids with benzonase. We then detail procedures for lyophilizing the ECM for long-term preservation and digesting the pancreatic ECM in pepsin-HCL to form a hydrogel.For complete details on the use and execution of this protocol, please refer to Tremmel et al.1 and Sackett et al.2 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-bf5dca8d64334f47b820d0ebcf32c172 |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-bf5dca8d64334f47b820d0ebcf32c1722025-08-20T03:31:37ZengElsevierSTAR Protocols2666-16672025-09-016310399710.1016/j.xpro.2025.103997Protocol for decellularizing and delipidizing human pancreatic tissue into native hydrogelConnie S. Chamberlain0Daniel M. Tremmel1Ayesha Palwasha Khan2Ellen Abad Santos3Deep Kapadia4Caterra Leavens5Katherine M. Gorski6Rawan Hamadeh7Austin K. Feeney8Jon Odorico9Sara D. Sackett10Division of Transplantation, Department of Surgery, University of Wisconsin–Madison, Madison, WI 53705, USADivision of Transplantation, Department of Surgery, University of Wisconsin–Madison, Madison, WI 53705, USA; Corresponding authorDivision of Transplantation, Department of Surgery, University of Wisconsin–Madison, Madison, WI 53705, USADivision of Transplantation, Department of Surgery, University of Wisconsin–Madison, Madison, WI 53705, USADivision of Transplantation, Department of Surgery, University of Wisconsin–Madison, Madison, WI 53705, USADivision of Transplantation, Department of Surgery, University of Wisconsin–Madison, Madison, WI 53705, USADivision of Transplantation, Department of Surgery, University of Wisconsin–Madison, Madison, WI 53705, USADivision of Transplantation, Department of Surgery, University of Wisconsin–Madison, Madison, WI 53705, USADivision of Transplantation, Department of Surgery, University of Wisconsin–Madison, Madison, WI 53705, USADivision of Transplantation, Department of Surgery, University of Wisconsin–Madison, Madison, WI 53705, USA; Corresponding authorDivision of Transplantation, Department of Surgery, University of Wisconsin–Madison, Madison, WI 53705, USA; Corresponding authorSummary: We present a protocol for transforming human pancreatic tissue into a decellularized and delipidized extracellular matrix (ECM) for hydrogel/scaffold applications. We describe steps for decellularization and delipidization using sodium deoxycholate and sequential ethanol, acetone, and hexane washes and removal of nucleic acids with benzonase. We then detail procedures for lyophilizing the ECM for long-term preservation and digesting the pancreatic ECM in pepsin-HCL to form a hydrogel.For complete details on the use and execution of this protocol, please refer to Tremmel et al.1 and Sackett et al.2 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725004034OrganoidsTissue EngineeringBiotechnology and bioengineering |
| spellingShingle | Connie S. Chamberlain Daniel M. Tremmel Ayesha Palwasha Khan Ellen Abad Santos Deep Kapadia Caterra Leavens Katherine M. Gorski Rawan Hamadeh Austin K. Feeney Jon Odorico Sara D. Sackett Protocol for decellularizing and delipidizing human pancreatic tissue into native hydrogel STAR Protocols Organoids Tissue Engineering Biotechnology and bioengineering |
| title | Protocol for decellularizing and delipidizing human pancreatic tissue into native hydrogel |
| title_full | Protocol for decellularizing and delipidizing human pancreatic tissue into native hydrogel |
| title_fullStr | Protocol for decellularizing and delipidizing human pancreatic tissue into native hydrogel |
| title_full_unstemmed | Protocol for decellularizing and delipidizing human pancreatic tissue into native hydrogel |
| title_short | Protocol for decellularizing and delipidizing human pancreatic tissue into native hydrogel |
| title_sort | protocol for decellularizing and delipidizing human pancreatic tissue into native hydrogel |
| topic | Organoids Tissue Engineering Biotechnology and bioengineering |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725004034 |
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