The small non-coding RNA response to virus infection in the Leishmania vector Lutzomyia longipalpis.
Sandflies are well known vectors for Leishmania but also transmit a number of arthropod-borne viruses (arboviruses). Few studies have addressed the interaction between sandflies and arboviruses. RNA interference (RNAi) mechanisms utilize small non-coding RNAs to regulate different aspects of host-pa...
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| _version_ | 1850231105810071552 |
|---|---|
| author | Flávia Viana Ferreira Eric Roberto Guimarães Rocha Aguiar Roenick Proveti Olmo Karla Pollyanna Vieira de Oliveira Emanuele Guimarães Silva Maurício Roberto Viana Sant'Anna Nelder de Figueiredo Gontijo Erna Geessien Kroon Jean Luc Imler João Trindade Marques |
| author_facet | Flávia Viana Ferreira Eric Roberto Guimarães Rocha Aguiar Roenick Proveti Olmo Karla Pollyanna Vieira de Oliveira Emanuele Guimarães Silva Maurício Roberto Viana Sant'Anna Nelder de Figueiredo Gontijo Erna Geessien Kroon Jean Luc Imler João Trindade Marques |
| author_sort | Flávia Viana Ferreira |
| collection | DOAJ |
| description | Sandflies are well known vectors for Leishmania but also transmit a number of arthropod-borne viruses (arboviruses). Few studies have addressed the interaction between sandflies and arboviruses. RNA interference (RNAi) mechanisms utilize small non-coding RNAs to regulate different aspects of host-pathogen interactions. The small interfering RNA (siRNA) pathway is a broad antiviral mechanism in insects. In addition, at least in mosquitoes, another RNAi mechanism mediated by PIWI interacting RNAs (piRNAs) is activated by viral infection. Finally, endogenous microRNAs (miRNA) may also regulate host immune responses. Here, we analyzed the small non-coding RNA response to Vesicular stomatitis virus (VSV) infection in the sandfly Lutzoymia longipalpis. We detected abundant production of virus-derived siRNAs after VSV infection in adult sandflies. However, there was no production of virus-derived piRNAs and only mild changes in the expression of vector miRNAs in response to infection. We also observed abundant production of virus-derived siRNAs against two other viruses in Lutzomyia Lulo cells. Together, our results suggest that the siRNA but not the piRNA pathway mediates an antiviral response in sandflies. In agreement with this hypothesis, pre-treatment of cells with dsRNA against VSV was able to inhibit viral replication while knock-down of the central siRNA component, Argonaute-2, led to increased virus levels. Our work begins to elucidate the role of RNAi mechanisms in the interaction between L. longipalpis and viruses and should also open the way for studies with other sandfly-borne pathogens. |
| format | Article |
| id | doaj-art-bf4fa07d75fa4dce98f287aa627c6c10 |
| institution | OA Journals |
| issn | 1935-2727 1935-2735 |
| language | English |
| publishDate | 2018-06-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj-art-bf4fa07d75fa4dce98f287aa627c6c102025-08-20T02:03:39ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352018-06-01126e000656910.1371/journal.pntd.0006569The small non-coding RNA response to virus infection in the Leishmania vector Lutzomyia longipalpis.Flávia Viana FerreiraEric Roberto Guimarães Rocha AguiarRoenick Proveti OlmoKarla Pollyanna Vieira de OliveiraEmanuele Guimarães SilvaMaurício Roberto Viana Sant'AnnaNelder de Figueiredo GontijoErna Geessien KroonJean Luc ImlerJoão Trindade MarquesSandflies are well known vectors for Leishmania but also transmit a number of arthropod-borne viruses (arboviruses). Few studies have addressed the interaction between sandflies and arboviruses. RNA interference (RNAi) mechanisms utilize small non-coding RNAs to regulate different aspects of host-pathogen interactions. The small interfering RNA (siRNA) pathway is a broad antiviral mechanism in insects. In addition, at least in mosquitoes, another RNAi mechanism mediated by PIWI interacting RNAs (piRNAs) is activated by viral infection. Finally, endogenous microRNAs (miRNA) may also regulate host immune responses. Here, we analyzed the small non-coding RNA response to Vesicular stomatitis virus (VSV) infection in the sandfly Lutzoymia longipalpis. We detected abundant production of virus-derived siRNAs after VSV infection in adult sandflies. However, there was no production of virus-derived piRNAs and only mild changes in the expression of vector miRNAs in response to infection. We also observed abundant production of virus-derived siRNAs against two other viruses in Lutzomyia Lulo cells. Together, our results suggest that the siRNA but not the piRNA pathway mediates an antiviral response in sandflies. In agreement with this hypothesis, pre-treatment of cells with dsRNA against VSV was able to inhibit viral replication while knock-down of the central siRNA component, Argonaute-2, led to increased virus levels. Our work begins to elucidate the role of RNAi mechanisms in the interaction between L. longipalpis and viruses and should also open the way for studies with other sandfly-borne pathogens.https://storage.googleapis.com/plos-corpus-prod/10.1371/journal.pntd.0006569/2/pntd.0006569.pdf?X-Goog-Algorithm=GOOG4-RSA-SHA256&X-Goog-Credential=wombat-sa%40plos-prod.iam.gserviceaccount.com%2F20210222%2Fauto%2Fstorage%2Fgoog4_request&X-Goog-Date=20210222T093041Z&X-Goog-Expires=3600&X-Goog-SignedHeaders=host&X-Goog-Signature=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 |
| spellingShingle | Flávia Viana Ferreira Eric Roberto Guimarães Rocha Aguiar Roenick Proveti Olmo Karla Pollyanna Vieira de Oliveira Emanuele Guimarães Silva Maurício Roberto Viana Sant'Anna Nelder de Figueiredo Gontijo Erna Geessien Kroon Jean Luc Imler João Trindade Marques The small non-coding RNA response to virus infection in the Leishmania vector Lutzomyia longipalpis. PLoS Neglected Tropical Diseases |
| title | The small non-coding RNA response to virus infection in the Leishmania vector Lutzomyia longipalpis. |
| title_full | The small non-coding RNA response to virus infection in the Leishmania vector Lutzomyia longipalpis. |
| title_fullStr | The small non-coding RNA response to virus infection in the Leishmania vector Lutzomyia longipalpis. |
| title_full_unstemmed | The small non-coding RNA response to virus infection in the Leishmania vector Lutzomyia longipalpis. |
| title_short | The small non-coding RNA response to virus infection in the Leishmania vector Lutzomyia longipalpis. |
| title_sort | small non coding rna response to virus infection in the leishmania vector lutzomyia longipalpis |
| url | https://storage.googleapis.com/plos-corpus-prod/10.1371/journal.pntd.0006569/2/pntd.0006569.pdf?X-Goog-Algorithm=GOOG4-RSA-SHA256&X-Goog-Credential=wombat-sa%40plos-prod.iam.gserviceaccount.com%2F20210222%2Fauto%2Fstorage%2Fgoog4_request&X-Goog-Date=20210222T093041Z&X-Goog-Expires=3600&X-Goog-SignedHeaders=host&X-Goog-Signature=73580c3b3f93a8f43325997a8b7b68a51fe13b923ec31d631fc707d251adc3dd3d011096585240899315b668d66b0a87f8f98563936b519695f9ac847165a5cd478eb03dca1ca9898498d426cf457b3e160a412fe9f0b7cf7007e741a98234a3cd165cc53189c0bcc59e973e9ec1948c126d28b08bb89bb6ca978180a4ae4c16db650cc157a6be988e458a1284605bcb31ece083479fa99201b94a11b2b7d79539e79ca3846dcb34f5bc28ddf908fadb7e2a20a206acea3a81f544aab7f30b332d97adbeb343dcecdd2f2481013679cd9be362839ba1d4cf229e66fbceada9ce2dbaa1b4c4bfe7b329b0124ceac15dd46573164c4c0ae84ca3755a2cfc39acea |
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