Assessing the impact of resistance training on renal function of female wistar rats under cross-sex hormone therapy
IntroductionCross-hormone therapy (CHT) is commonly used in the gender-affirming process, with testosterone being administered to trans men to develop secondary masculine characteristics. In experimental models replicating this condition, CHT has been associated with increased plasma creatinine leve...
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Frontiers Media S.A.
2025-05-01
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2025.1543077/full |
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| author | Isadora Gonçalves Almeida Isabela Borges M. Silveira Isabela Borges M. Silveira Emily Rocha Cordeiro Letícia Maria Monteiro Letícia Maria Monteiro Nathalia Beserra da Silva Nathalia Beserra da Silva Rogerio Argeri Rogerio Argeri Debora C. K. Lichtenecker Magnus R. Dias da Silva Magnus R. Dias da Silva Guiomar Nascimento Gomes |
| author_facet | Isadora Gonçalves Almeida Isabela Borges M. Silveira Isabela Borges M. Silveira Emily Rocha Cordeiro Letícia Maria Monteiro Letícia Maria Monteiro Nathalia Beserra da Silva Nathalia Beserra da Silva Rogerio Argeri Rogerio Argeri Debora C. K. Lichtenecker Magnus R. Dias da Silva Magnus R. Dias da Silva Guiomar Nascimento Gomes |
| author_sort | Isadora Gonçalves Almeida |
| collection | DOAJ |
| description | IntroductionCross-hormone therapy (CHT) is commonly used in the gender-affirming process, with testosterone being administered to trans men to develop secondary masculine characteristics. In experimental models replicating this condition, CHT has been associated with increased plasma creatinine levels and renal morphological changes. Given benefits of physical exercise, this study aimed to evaluate whether resistance training could mitigate CHT-induced renal alterations.ObjectivesTo investigate the impact of resistance training combined with CHT on blood pressure and renal morphology and function.MethodsTwo-month-old female Wistar rats were divided into four groups: FSV–sedentary rats treated with vehicle (vegetable oil); FSH–sedentary rats treated with CHT; FEV–exercised rats treated with vehicle; and FEH–exercised rats treated with CHT. CHT was administered via testosterone cypionate (3.0 mg/kg, intramuscularly) every 10 days for 8 weeks. Exercise groups underwent progressive resistance training using a vertical climbing ladder five times per week for 6 weeks. At the end of the protocol, the animals were placed in metabolic cages for urine collection, followed by blood sampling for biochemical analysis.ResultsTestosterone-treated groups showed increased plasma creatinine levels, though urea concentrations were unchanged. Plasma sodium concentration was elevated, and sodium excretion was reduced in the sedentary testosterone-treated group. Morphological analysis revealed that resistance exercise reduced macrophage infiltration, lowered the number of PCNA-positive cells in kidney tissue, and decreased glomerular tubularization in the kidney.ConclusionTestosterone-based CHT in female rats induces renal functional alterations, but resistance exercise effectively attenuates these effects by reducing macrophage infiltration, cell proliferation, and glomerular changes. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-05-01 |
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| series | Frontiers in Physiology |
| spelling | doaj-art-bf47a01453d048bd84416b729ba71dd72025-08-20T03:47:49ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2025-05-011610.3389/fphys.2025.15430771543077Assessing the impact of resistance training on renal function of female wistar rats under cross-sex hormone therapyIsadora Gonçalves Almeida0Isabela Borges M. Silveira1Isabela Borges M. Silveira2Emily Rocha Cordeiro3Letícia Maria Monteiro4Letícia Maria Monteiro5Nathalia Beserra da Silva6Nathalia Beserra da Silva7Rogerio Argeri8Rogerio Argeri9Debora C. K. Lichtenecker10Magnus R. Dias da Silva11Magnus R. Dias da Silva12Guiomar Nascimento Gomes13Laboratory of Renal Physiology Department of Physiology, Escola Paulista de Medicina, Universidade Federal de São Paulo (EPM/Unifesp), São Paulo, BrazilLaboratory of Renal Physiology Department of Physiology, Escola Paulista de Medicina, Universidade Federal de São Paulo (EPM/Unifesp), São Paulo, BrazilPostgraduate Program in Translational Medicine, Department of Medicine, Escola Paulista de Medicina, Federal University of São Paulo, São Paulo, BrazilLaboratory of Renal Physiology Department of Physiology, Escola Paulista de Medicina, Universidade Federal de São Paulo (EPM/Unifesp), São Paulo, BrazilLaboratory of Renal Physiology Department of Physiology, Escola Paulista de Medicina, Universidade Federal de São Paulo (EPM/Unifesp), São Paulo, BrazilPostgraduate Program in Translational Medicine, Department of Medicine, Escola Paulista de Medicina, Federal University of São Paulo, São Paulo, BrazilLaboratory of Renal Physiology Department of Physiology, Escola Paulista de Medicina, Universidade Federal de São Paulo (EPM/Unifesp), São Paulo, BrazilPostgraduate Program in Translational Medicine, Department of Medicine, Escola Paulista de Medicina, Federal University of São Paulo, São Paulo, BrazilLaboratory of Renal Physiology Department of Physiology, Escola Paulista de Medicina, Universidade Federal de São Paulo (EPM/Unifesp), São Paulo, BrazilPostgraduate Program in Translational Medicine, Department of Medicine, Escola Paulista de Medicina, Federal University of São Paulo, São Paulo, BrazilLaboratory of Renal Physiology Department of Physiology, Escola Paulista de Medicina, Universidade Federal de São Paulo (EPM/Unifesp), São Paulo, BrazilLaboratory of Molecular and Translational Endocrinology (LEMT), Endocrinology Division, Department of Medicine, Escola Paulista de Medicina, Universidade Federal de São Paulo (EPM/Unifesp), São Paulo, BrazilTrans Care Outpatient Clinics; Núcleo de Estudos, Pesquisa, Extensão e Assistência à Pessoa Trans Professor Roberto Farina, Universidade Federal de São Paulo (Núcleo TransUnifesp), São Paulo, BrazilLaboratory of Renal Physiology Department of Physiology, Escola Paulista de Medicina, Universidade Federal de São Paulo (EPM/Unifesp), São Paulo, BrazilIntroductionCross-hormone therapy (CHT) is commonly used in the gender-affirming process, with testosterone being administered to trans men to develop secondary masculine characteristics. In experimental models replicating this condition, CHT has been associated with increased plasma creatinine levels and renal morphological changes. Given benefits of physical exercise, this study aimed to evaluate whether resistance training could mitigate CHT-induced renal alterations.ObjectivesTo investigate the impact of resistance training combined with CHT on blood pressure and renal morphology and function.MethodsTwo-month-old female Wistar rats were divided into four groups: FSV–sedentary rats treated with vehicle (vegetable oil); FSH–sedentary rats treated with CHT; FEV–exercised rats treated with vehicle; and FEH–exercised rats treated with CHT. CHT was administered via testosterone cypionate (3.0 mg/kg, intramuscularly) every 10 days for 8 weeks. Exercise groups underwent progressive resistance training using a vertical climbing ladder five times per week for 6 weeks. At the end of the protocol, the animals were placed in metabolic cages for urine collection, followed by blood sampling for biochemical analysis.ResultsTestosterone-treated groups showed increased plasma creatinine levels, though urea concentrations were unchanged. Plasma sodium concentration was elevated, and sodium excretion was reduced in the sedentary testosterone-treated group. Morphological analysis revealed that resistance exercise reduced macrophage infiltration, lowered the number of PCNA-positive cells in kidney tissue, and decreased glomerular tubularization in the kidney.ConclusionTestosterone-based CHT in female rats induces renal functional alterations, but resistance exercise effectively attenuates these effects by reducing macrophage infiltration, cell proliferation, and glomerular changes.https://www.frontiersin.org/articles/10.3389/fphys.2025.1543077/fullcross-hormone-therapytestosteronephysical exercise (EX)resistance trainingrenal function |
| spellingShingle | Isadora Gonçalves Almeida Isabela Borges M. Silveira Isabela Borges M. Silveira Emily Rocha Cordeiro Letícia Maria Monteiro Letícia Maria Monteiro Nathalia Beserra da Silva Nathalia Beserra da Silva Rogerio Argeri Rogerio Argeri Debora C. K. Lichtenecker Magnus R. Dias da Silva Magnus R. Dias da Silva Guiomar Nascimento Gomes Assessing the impact of resistance training on renal function of female wistar rats under cross-sex hormone therapy Frontiers in Physiology cross-hormone-therapy testosterone physical exercise (EX) resistance training renal function |
| title | Assessing the impact of resistance training on renal function of female wistar rats under cross-sex hormone therapy |
| title_full | Assessing the impact of resistance training on renal function of female wistar rats under cross-sex hormone therapy |
| title_fullStr | Assessing the impact of resistance training on renal function of female wistar rats under cross-sex hormone therapy |
| title_full_unstemmed | Assessing the impact of resistance training on renal function of female wistar rats under cross-sex hormone therapy |
| title_short | Assessing the impact of resistance training on renal function of female wistar rats under cross-sex hormone therapy |
| title_sort | assessing the impact of resistance training on renal function of female wistar rats under cross sex hormone therapy |
| topic | cross-hormone-therapy testosterone physical exercise (EX) resistance training renal function |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2025.1543077/full |
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