Detection of CWD in cervids by RT-QuIC assay of third eyelids.
The diagnosis of chronic wasting disease (CWD) relies on demonstration of the disease-associated misfolded CWD prion protein (PrPCWD) in brain or retropharyngeal lymph node tissue by immunodetection methods, e.g. ELISA and immunohistochemistry (IHC). The success of these methods relies on a quality...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2019-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0221654&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849317020590407680 |
|---|---|
| author | Sarah K Cooper Clare E Hoover Davin M Henderson Nicholas J Haley Candace K Mathiason Edward A Hoover |
| author_facet | Sarah K Cooper Clare E Hoover Davin M Henderson Nicholas J Haley Candace K Mathiason Edward A Hoover |
| author_sort | Sarah K Cooper |
| collection | DOAJ |
| description | The diagnosis of chronic wasting disease (CWD) relies on demonstration of the disease-associated misfolded CWD prion protein (PrPCWD) in brain or retropharyngeal lymph node tissue by immunodetection methods, e.g. ELISA and immunohistochemistry (IHC). The success of these methods relies on a quality sample of tissues, which requires both anatomical knowledge and considerable dissection to collect. As the prevalence of CWD continues to increase globally, the development of fast and cost-effective methods to detect the disease is vital to facilitate CWD detection and surveillance. To address these issues, we have evaluated third eyelids from CWD-infected deer and elk using real-time quaking induced conversion (RT-QuIC). We identified prion seeding activity in third eyelids in 24 of 25 (96%) CWD-infected white-tailed deer (Odocoileus virginianus). We detected RT-QuIC positivity in the third eyelid as early as 1 month after experimental CWD exposure. In addition, we identified prion seeding activity in third eyelids of 18 of 25 (72%) naturally exposed asymptomatic CWD-positive rocky mountain elk (Cervus canadensis nelson). We compared CWD detection by RT-QuIC and IHC in third eyelid, retropharyngeal lymph node, and brain in 10 deer in early symptomatic stage of disease. IHC detected PrPCWD deposition in third eyelid lymphoid follicles in 5 of 10 deer (50%) whereas third eyelids of all 10 animals were positive by RT-QuIC. This difference reflected in part a lower requirement for lymphoid follicle presence for seeding activity detection by RT-QuIC. In conclusion, RT-QuIC analysis of the third eyelid, an easily accessed tissue, has potential to advance CWD detection and testing compliance. |
| format | Article |
| id | doaj-art-bf4290ff7f0149f4b517066f4f00a542 |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-bf4290ff7f0149f4b517066f4f00a5422025-08-20T03:51:24ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01148e022165410.1371/journal.pone.0221654Detection of CWD in cervids by RT-QuIC assay of third eyelids.Sarah K CooperClare E HooverDavin M HendersonNicholas J HaleyCandace K MathiasonEdward A HooverThe diagnosis of chronic wasting disease (CWD) relies on demonstration of the disease-associated misfolded CWD prion protein (PrPCWD) in brain or retropharyngeal lymph node tissue by immunodetection methods, e.g. ELISA and immunohistochemistry (IHC). The success of these methods relies on a quality sample of tissues, which requires both anatomical knowledge and considerable dissection to collect. As the prevalence of CWD continues to increase globally, the development of fast and cost-effective methods to detect the disease is vital to facilitate CWD detection and surveillance. To address these issues, we have evaluated third eyelids from CWD-infected deer and elk using real-time quaking induced conversion (RT-QuIC). We identified prion seeding activity in third eyelids in 24 of 25 (96%) CWD-infected white-tailed deer (Odocoileus virginianus). We detected RT-QuIC positivity in the third eyelid as early as 1 month after experimental CWD exposure. In addition, we identified prion seeding activity in third eyelids of 18 of 25 (72%) naturally exposed asymptomatic CWD-positive rocky mountain elk (Cervus canadensis nelson). We compared CWD detection by RT-QuIC and IHC in third eyelid, retropharyngeal lymph node, and brain in 10 deer in early symptomatic stage of disease. IHC detected PrPCWD deposition in third eyelid lymphoid follicles in 5 of 10 deer (50%) whereas third eyelids of all 10 animals were positive by RT-QuIC. This difference reflected in part a lower requirement for lymphoid follicle presence for seeding activity detection by RT-QuIC. In conclusion, RT-QuIC analysis of the third eyelid, an easily accessed tissue, has potential to advance CWD detection and testing compliance.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0221654&type=printable |
| spellingShingle | Sarah K Cooper Clare E Hoover Davin M Henderson Nicholas J Haley Candace K Mathiason Edward A Hoover Detection of CWD in cervids by RT-QuIC assay of third eyelids. PLoS ONE |
| title | Detection of CWD in cervids by RT-QuIC assay of third eyelids. |
| title_full | Detection of CWD in cervids by RT-QuIC assay of third eyelids. |
| title_fullStr | Detection of CWD in cervids by RT-QuIC assay of third eyelids. |
| title_full_unstemmed | Detection of CWD in cervids by RT-QuIC assay of third eyelids. |
| title_short | Detection of CWD in cervids by RT-QuIC assay of third eyelids. |
| title_sort | detection of cwd in cervids by rt quic assay of third eyelids |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0221654&type=printable |
| work_keys_str_mv | AT sarahkcooper detectionofcwdincervidsbyrtquicassayofthirdeyelids AT clareehoover detectionofcwdincervidsbyrtquicassayofthirdeyelids AT davinmhenderson detectionofcwdincervidsbyrtquicassayofthirdeyelids AT nicholasjhaley detectionofcwdincervidsbyrtquicassayofthirdeyelids AT candacekmathiason detectionofcwdincervidsbyrtquicassayofthirdeyelids AT edwardahoover detectionofcwdincervidsbyrtquicassayofthirdeyelids |