Profile of tRNA-derived short non-coding RNAs during monocyte differentiation and their role in macrophage survival
Monocytes, a type of leukocytes, are key players in immune responses, transitioning to macrophages at infection sites. Differentiation, which is crucial for macrophage survival and resistance to apoptosis, is a tightly regulated process. Based on our earlier findings implicating the 5'-fragment...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | RNA Biology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/15476286.2025.2525886 |
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| author | Anahita Jayaram Sanjeev Galande Soroush Sharbati Deepak Deshpande Kamlesh Pawar |
| author_facet | Anahita Jayaram Sanjeev Galande Soroush Sharbati Deepak Deshpande Kamlesh Pawar |
| author_sort | Anahita Jayaram |
| collection | DOAJ |
| description | Monocytes, a type of leukocytes, are key players in immune responses, transitioning to macrophages at infection sites. Differentiation, which is crucial for macrophage survival and resistance to apoptosis, is a tightly regulated process. Based on our earlier findings implicating the 5'-fragment of tRNAHisGUG (5'-HisGUG) in macrophage biology, we specifically focused on these fragments to investigate its regulation during monocyte-to-macrophage differentiation and its role in macrophage survival. We performed small RNA sequencing during monocyte-to-macrophage differentiation using THP-1 cells and identified 5'-HisGUG as a prominently regulated tDR. Notably, transfection of 5'-HisGUG in macrophages decreased survival under apoptotic stress. We propose that 5'-HisGUG can also act as an obstructive RNA (oRNA), and its reduction is critical for macrophage survival under stress. |
| format | Article |
| id | doaj-art-bf40460865d841c9977252c6ca849e9d |
| institution | DOAJ |
| issn | 1547-6286 1555-8584 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | RNA Biology |
| spelling | doaj-art-bf40460865d841c9977252c6ca849e9d2025-08-20T02:44:50ZengTaylor & Francis GroupRNA Biology1547-62861555-85842025-12-012211910.1080/15476286.2025.2525886Profile of tRNA-derived short non-coding RNAs during monocyte differentiation and their role in macrophage survivalAnahita Jayaram0Sanjeev Galande1Soroush Sharbati2Deepak Deshpande3Kamlesh Pawar4Centre of Excellence in Epigenetics (CoEE), Department of Life Sciences, School of Natural Science, Shiv Nadar Institution of Eminence Deemed to be University, Delhi NCR, Greater Noida, IndiaCentre of Excellence in Epigenetics (CoEE), Department of Life Sciences, School of Natural Science, Shiv Nadar Institution of Eminence Deemed to be University, Delhi NCR, Greater Noida, IndiaInstitute of Veterinary Biochemistry, School of Veterinary Medicine, Berlin, Germany, Freie Universität Berlin institutionCenter for Translational Medicine, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, PA, USACentre of Excellence in Epigenetics (CoEE), Department of Life Sciences, School of Natural Science, Shiv Nadar Institution of Eminence Deemed to be University, Delhi NCR, Greater Noida, IndiaMonocytes, a type of leukocytes, are key players in immune responses, transitioning to macrophages at infection sites. Differentiation, which is crucial for macrophage survival and resistance to apoptosis, is a tightly regulated process. Based on our earlier findings implicating the 5'-fragment of tRNAHisGUG (5'-HisGUG) in macrophage biology, we specifically focused on these fragments to investigate its regulation during monocyte-to-macrophage differentiation and its role in macrophage survival. We performed small RNA sequencing during monocyte-to-macrophage differentiation using THP-1 cells and identified 5'-HisGUG as a prominently regulated tDR. Notably, transfection of 5'-HisGUG in macrophages decreased survival under apoptotic stress. We propose that 5'-HisGUG can also act as an obstructive RNA (oRNA), and its reduction is critical for macrophage survival under stress.https://www.tandfonline.com/doi/10.1080/15476286.2025.2525886MonocytemacrophagedifferentiationncRNAtRNA-fragmentsapoptosis |
| spellingShingle | Anahita Jayaram Sanjeev Galande Soroush Sharbati Deepak Deshpande Kamlesh Pawar Profile of tRNA-derived short non-coding RNAs during monocyte differentiation and their role in macrophage survival RNA Biology Monocyte macrophage differentiation ncRNA tRNA-fragments apoptosis |
| title | Profile of tRNA-derived short non-coding RNAs during monocyte differentiation and their role in macrophage survival |
| title_full | Profile of tRNA-derived short non-coding RNAs during monocyte differentiation and their role in macrophage survival |
| title_fullStr | Profile of tRNA-derived short non-coding RNAs during monocyte differentiation and their role in macrophage survival |
| title_full_unstemmed | Profile of tRNA-derived short non-coding RNAs during monocyte differentiation and their role in macrophage survival |
| title_short | Profile of tRNA-derived short non-coding RNAs during monocyte differentiation and their role in macrophage survival |
| title_sort | profile of trna derived short non coding rnas during monocyte differentiation and their role in macrophage survival |
| topic | Monocyte macrophage differentiation ncRNA tRNA-fragments apoptosis |
| url | https://www.tandfonline.com/doi/10.1080/15476286.2025.2525886 |
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