Tissue Adequacy and Diagnostic Yield Assessment in Malignant Lymph Nodes Using Endobronchial Ultrasound (EBUS)-Guided Miniforcep Biopsy vs. EBUS-Guided Transbronchial Needle Aspiration

Background Endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) is a predominantly used method for lymph node (LN) metastasis assessment. This study aims to identify tissue adequacy improvement with the addition of EBUS-guided miniforcep biopsy (EBUS-MFB) to EBUS-TBNA in samp...

Full description

Saved in:
Bibliographic Details
Main Authors: Pipu Tavornshevin, Poonchavist Chantranuwatana, Vorawut Thanthitaweewat, Virissorn Wongsrichanalai, Thitiwat Sriprasart, Nophol Leelayuwatanakul
Format: Article
Language:English
Published: The Korean Academy of Tuberculosis and Respiratory Diseases 2025-07-01
Series:Tuberculosis and Respiratory Diseases
Subjects:
Online Access:http://e-trd.org/upload/pdf/trd-2024-0134.pdf
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849416266119380992
author Pipu Tavornshevin
Poonchavist Chantranuwatana
Vorawut Thanthitaweewat
Virissorn Wongsrichanalai
Thitiwat Sriprasart
Nophol Leelayuwatanakul
author_facet Pipu Tavornshevin
Poonchavist Chantranuwatana
Vorawut Thanthitaweewat
Virissorn Wongsrichanalai
Thitiwat Sriprasart
Nophol Leelayuwatanakul
author_sort Pipu Tavornshevin
collection DOAJ
description Background Endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) is a predominantly used method for lymph node (LN) metastasis assessment. This study aims to identify tissue adequacy improvement with the addition of EBUS-guided miniforcep biopsy (EBUS-MFB) to EBUS-TBNA in sampling LNs. Methods We assessed tissue adequacy in patients with mediastinal and hilar lymphadenopathy, comparing the combination of EBUS-MFB and EBUS-TBNA with EBUS-TBNA alone. EBUS-MFB was performed with the guide sheath (GS) dilatation technique. Tissue adequacy was a tumor cell count (TCC) of >100 and neoplastic cell neoplastic cell estimate of >25%. Further, we reported the diagnostic yield, tumor cell characteristics, and safety outcomes. Results Among 69 patients (74 nodes), malignant diseases were diagnosed in 41 nodes using both techniques. Tissue adequacy with EBUS-TBNA (93.8% in 30/32 nodes) was comparable with the combined group (96.9% in 31/32 nodes, p=0.317). EBUS-TBNA yielded higher TCC (84.4% with >1,000 cells) than EBUS-MFB (53.1%, p=0.004). The combined approach significantly improved the diagnostic yield in non-malignant diseases compared with EBUS-TBNA alone (97% vs. 78.8%, p=0.014). Of the 32 nodes, 20 demonstrated discordant results between EBUS-TBNA and EBUS-MFB, with EBUS-MFB correctly diagnosing six nodes that EBUS-TBNA misdiagnosed. The complication rate was low (2.9%) with only minor bleeding reported. Conclusion EBUS-TBNA alone and the combination of EBUS-MFB and EBUS-TBNA demonstrated comparable tissue adequacy, with EBUS-TBNA exhibiting better specimen characteristics, potentially sufficient for various molecular analyses. The addition of EBUS-MFB, performed using the GS-dilatation technique, to EBUS-TBNA improved the diagnostic yield and proved to be a safe and efficient approach, particularly in non-malignant diseases.
format Article
id doaj-art-bf33347d58cf4e50bf71fbb54339d794
institution Kabale University
issn 1738-3536
2005-6184
language English
publishDate 2025-07-01
publisher The Korean Academy of Tuberculosis and Respiratory Diseases
record_format Article
series Tuberculosis and Respiratory Diseases
spelling doaj-art-bf33347d58cf4e50bf71fbb54339d7942025-08-20T03:33:14ZengThe Korean Academy of Tuberculosis and Respiratory DiseasesTuberculosis and Respiratory Diseases1738-35362005-61842025-07-0188354555610.4046/trd.2024.01344941Tissue Adequacy and Diagnostic Yield Assessment in Malignant Lymph Nodes Using Endobronchial Ultrasound (EBUS)-Guided Miniforcep Biopsy vs. EBUS-Guided Transbronchial Needle AspirationPipu Tavornshevin0Poonchavist Chantranuwatana1Vorawut Thanthitaweewat2Virissorn Wongsrichanalai3Thitiwat Sriprasart4Nophol Leelayuwatanakul5 Division of Pulmonary and Critical Care Medicine, Department of Medicine, Chulalongkorn University, Bangkok, Thailand Department of Pathology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand Division of Pulmonary and Critical Care Medicine, Department of Medicine, Chulalongkorn University, Bangkok, Thailand Division of Pulmonary and Critical Care Medicine, Department of Medicine, Chulalongkorn University, Bangkok, Thailand Division of Pulmonary and Critical Care Medicine, Department of Medicine, Chulalongkorn University, Bangkok, Thailand Division of Pulmonary and Critical Care Medicine, Department of Medicine, Chulalongkorn University, Bangkok, ThailandBackground Endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) is a predominantly used method for lymph node (LN) metastasis assessment. This study aims to identify tissue adequacy improvement with the addition of EBUS-guided miniforcep biopsy (EBUS-MFB) to EBUS-TBNA in sampling LNs. Methods We assessed tissue adequacy in patients with mediastinal and hilar lymphadenopathy, comparing the combination of EBUS-MFB and EBUS-TBNA with EBUS-TBNA alone. EBUS-MFB was performed with the guide sheath (GS) dilatation technique. Tissue adequacy was a tumor cell count (TCC) of >100 and neoplastic cell neoplastic cell estimate of >25%. Further, we reported the diagnostic yield, tumor cell characteristics, and safety outcomes. Results Among 69 patients (74 nodes), malignant diseases were diagnosed in 41 nodes using both techniques. Tissue adequacy with EBUS-TBNA (93.8% in 30/32 nodes) was comparable with the combined group (96.9% in 31/32 nodes, p=0.317). EBUS-TBNA yielded higher TCC (84.4% with >1,000 cells) than EBUS-MFB (53.1%, p=0.004). The combined approach significantly improved the diagnostic yield in non-malignant diseases compared with EBUS-TBNA alone (97% vs. 78.8%, p=0.014). Of the 32 nodes, 20 demonstrated discordant results between EBUS-TBNA and EBUS-MFB, with EBUS-MFB correctly diagnosing six nodes that EBUS-TBNA misdiagnosed. The complication rate was low (2.9%) with only minor bleeding reported. Conclusion EBUS-TBNA alone and the combination of EBUS-MFB and EBUS-TBNA demonstrated comparable tissue adequacy, with EBUS-TBNA exhibiting better specimen characteristics, potentially sufficient for various molecular analyses. The addition of EBUS-MFB, performed using the GS-dilatation technique, to EBUS-TBNA improved the diagnostic yield and proved to be a safe and efficient approach, particularly in non-malignant diseases.http://e-trd.org/upload/pdf/trd-2024-0134.pdfendobronchial ultrasoundtransbronchial needle aspirationminiforcep biopsymediastinal and hilar lymphadenopathytissue adequacy
spellingShingle Pipu Tavornshevin
Poonchavist Chantranuwatana
Vorawut Thanthitaweewat
Virissorn Wongsrichanalai
Thitiwat Sriprasart
Nophol Leelayuwatanakul
Tissue Adequacy and Diagnostic Yield Assessment in Malignant Lymph Nodes Using Endobronchial Ultrasound (EBUS)-Guided Miniforcep Biopsy vs. EBUS-Guided Transbronchial Needle Aspiration
Tuberculosis and Respiratory Diseases
endobronchial ultrasound
transbronchial needle aspiration
miniforcep biopsy
mediastinal and hilar lymphadenopathy
tissue adequacy
title Tissue Adequacy and Diagnostic Yield Assessment in Malignant Lymph Nodes Using Endobronchial Ultrasound (EBUS)-Guided Miniforcep Biopsy vs. EBUS-Guided Transbronchial Needle Aspiration
title_full Tissue Adequacy and Diagnostic Yield Assessment in Malignant Lymph Nodes Using Endobronchial Ultrasound (EBUS)-Guided Miniforcep Biopsy vs. EBUS-Guided Transbronchial Needle Aspiration
title_fullStr Tissue Adequacy and Diagnostic Yield Assessment in Malignant Lymph Nodes Using Endobronchial Ultrasound (EBUS)-Guided Miniforcep Biopsy vs. EBUS-Guided Transbronchial Needle Aspiration
title_full_unstemmed Tissue Adequacy and Diagnostic Yield Assessment in Malignant Lymph Nodes Using Endobronchial Ultrasound (EBUS)-Guided Miniforcep Biopsy vs. EBUS-Guided Transbronchial Needle Aspiration
title_short Tissue Adequacy and Diagnostic Yield Assessment in Malignant Lymph Nodes Using Endobronchial Ultrasound (EBUS)-Guided Miniforcep Biopsy vs. EBUS-Guided Transbronchial Needle Aspiration
title_sort tissue adequacy and diagnostic yield assessment in malignant lymph nodes using endobronchial ultrasound ebus guided miniforcep biopsy vs ebus guided transbronchial needle aspiration
topic endobronchial ultrasound
transbronchial needle aspiration
miniforcep biopsy
mediastinal and hilar lymphadenopathy
tissue adequacy
url http://e-trd.org/upload/pdf/trd-2024-0134.pdf
work_keys_str_mv AT piputavornshevin tissueadequacyanddiagnosticyieldassessmentinmalignantlymphnodesusingendobronchialultrasoundebusguidedminiforcepbiopsyvsebusguidedtransbronchialneedleaspiration
AT poonchavistchantranuwatana tissueadequacyanddiagnosticyieldassessmentinmalignantlymphnodesusingendobronchialultrasoundebusguidedminiforcepbiopsyvsebusguidedtransbronchialneedleaspiration
AT vorawutthanthitaweewat tissueadequacyanddiagnosticyieldassessmentinmalignantlymphnodesusingendobronchialultrasoundebusguidedminiforcepbiopsyvsebusguidedtransbronchialneedleaspiration
AT virissornwongsrichanalai tissueadequacyanddiagnosticyieldassessmentinmalignantlymphnodesusingendobronchialultrasoundebusguidedminiforcepbiopsyvsebusguidedtransbronchialneedleaspiration
AT thitiwatsriprasart tissueadequacyanddiagnosticyieldassessmentinmalignantlymphnodesusingendobronchialultrasoundebusguidedminiforcepbiopsyvsebusguidedtransbronchialneedleaspiration
AT nopholleelayuwatanakul tissueadequacyanddiagnosticyieldassessmentinmalignantlymphnodesusingendobronchialultrasoundebusguidedminiforcepbiopsyvsebusguidedtransbronchialneedleaspiration