Three-Dimensional Aggregates Enhance the Therapeutic Effects of Adipose Mesenchymal Stem Cells for Ischemia-Reperfusion Induced Kidney Injury in Rats
It has been shown that administration of adipose derived mesenchymal stem cells (AdMSCs) enhanced structural and functional recovery of renal ischemia-reperfusion (IR) injury. Low engraftment of stem cells, however, limits the therapeutic effects of AdMSCs. The present study was designed to enhance...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/9062638 |
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| author | Xiaozhi Zhao Xuefeng Qiu Yanting Zhang Shiwei Zhang Xiaoping Gu Hongqian Guo |
| author_facet | Xiaozhi Zhao Xuefeng Qiu Yanting Zhang Shiwei Zhang Xiaoping Gu Hongqian Guo |
| author_sort | Xiaozhi Zhao |
| collection | DOAJ |
| description | It has been shown that administration of adipose derived mesenchymal stem cells (AdMSCs) enhanced structural and functional recovery of renal ischemia-reperfusion (IR) injury. Low engraftment of stem cells, however, limits the therapeutic effects of AdMSCs. The present study was designed to enhance the therapeutic effects of AdMSCs by delivering AdMSCs in a three-dimensional (3D) aggregates form. Microwell was used to produce 3D AdMSCs aggregates. In vitro data indicated that AdMSCs in 3D aggregates were less susceptible to oxidative and hypoxia stress induced by 200 μM peroxide and hypoxia/reoxygenation, respectively, compared with those cultured in two-dimensional (2D) monolayer. Furthermore, AdMSCs in 3D aggregates secreted more proangiogenic factors than those cultured in 2D monolayer. 2D AdMSCs or 3D AdMSCs aggregates were injected into renal cortex immediately after induction of renal IR injury. In vivo data revealed that 3D aggregates enhanced the effects of AdMSCs in recovering function and structure after renal IR injury. Improved grafted AdMSCs were observed in kidney injected with 3D aggregates compared with AdMSCs cultured in 2D monolayer. Our results demonstrated that 3D AdMSCs aggregated produced by microwell enhanced the retention and therapeutic effects of AdMSCs for renal IR injury. |
| format | Article |
| id | doaj-art-bf2e7bed3a044ffa85a91e06a3436402 |
| institution | DOAJ |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-bf2e7bed3a044ffa85a91e06a34364022025-08-20T03:23:07ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/90626389062638Three-Dimensional Aggregates Enhance the Therapeutic Effects of Adipose Mesenchymal Stem Cells for Ischemia-Reperfusion Induced Kidney Injury in RatsXiaozhi Zhao0Xuefeng Qiu1Yanting Zhang2Shiwei Zhang3Xiaoping Gu4Hongqian Guo5Department of Urology, Affiliated Drum Tower Hospital, School of Medicine, Nanjing University, Nanjing 210008, ChinaDepartment of Urology, Affiliated Drum Tower Hospital, School of Medicine, Nanjing University, Nanjing 210008, ChinaState Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, ChinaDepartment of Urology, Affiliated Drum Tower Hospital, School of Medicine, Nanjing University, Nanjing 210008, ChinaDepartment of Anesthesiology, Affiliated Drum Tower Hospital, School of Medicine, Nanjing University, Nanjing 210008, ChinaDepartment of Urology, Affiliated Drum Tower Hospital, School of Medicine, Nanjing University, Nanjing 210008, ChinaIt has been shown that administration of adipose derived mesenchymal stem cells (AdMSCs) enhanced structural and functional recovery of renal ischemia-reperfusion (IR) injury. Low engraftment of stem cells, however, limits the therapeutic effects of AdMSCs. The present study was designed to enhance the therapeutic effects of AdMSCs by delivering AdMSCs in a three-dimensional (3D) aggregates form. Microwell was used to produce 3D AdMSCs aggregates. In vitro data indicated that AdMSCs in 3D aggregates were less susceptible to oxidative and hypoxia stress induced by 200 μM peroxide and hypoxia/reoxygenation, respectively, compared with those cultured in two-dimensional (2D) monolayer. Furthermore, AdMSCs in 3D aggregates secreted more proangiogenic factors than those cultured in 2D monolayer. 2D AdMSCs or 3D AdMSCs aggregates were injected into renal cortex immediately after induction of renal IR injury. In vivo data revealed that 3D aggregates enhanced the effects of AdMSCs in recovering function and structure after renal IR injury. Improved grafted AdMSCs were observed in kidney injected with 3D aggregates compared with AdMSCs cultured in 2D monolayer. Our results demonstrated that 3D AdMSCs aggregated produced by microwell enhanced the retention and therapeutic effects of AdMSCs for renal IR injury.http://dx.doi.org/10.1155/2016/9062638 |
| spellingShingle | Xiaozhi Zhao Xuefeng Qiu Yanting Zhang Shiwei Zhang Xiaoping Gu Hongqian Guo Three-Dimensional Aggregates Enhance the Therapeutic Effects of Adipose Mesenchymal Stem Cells for Ischemia-Reperfusion Induced Kidney Injury in Rats Stem Cells International |
| title | Three-Dimensional Aggregates Enhance the Therapeutic Effects of Adipose Mesenchymal Stem Cells for Ischemia-Reperfusion Induced Kidney Injury in Rats |
| title_full | Three-Dimensional Aggregates Enhance the Therapeutic Effects of Adipose Mesenchymal Stem Cells for Ischemia-Reperfusion Induced Kidney Injury in Rats |
| title_fullStr | Three-Dimensional Aggregates Enhance the Therapeutic Effects of Adipose Mesenchymal Stem Cells for Ischemia-Reperfusion Induced Kidney Injury in Rats |
| title_full_unstemmed | Three-Dimensional Aggregates Enhance the Therapeutic Effects of Adipose Mesenchymal Stem Cells for Ischemia-Reperfusion Induced Kidney Injury in Rats |
| title_short | Three-Dimensional Aggregates Enhance the Therapeutic Effects of Adipose Mesenchymal Stem Cells for Ischemia-Reperfusion Induced Kidney Injury in Rats |
| title_sort | three dimensional aggregates enhance the therapeutic effects of adipose mesenchymal stem cells for ischemia reperfusion induced kidney injury in rats |
| url | http://dx.doi.org/10.1155/2016/9062638 |
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