Involvement of liver kinase B1 in the ameliorative action of epigallocatechin-3-gallate against MAFLD progression in mice

Metabolic dysfunction-associated fatty liver disease (MAFLD) is characterized by intrahepatic fat accumulation in individuals without a history of excessive alcohol consumption. This study investigated the effects of epigallocatechin-3-gallate (EGCG) on reducing MAFLD severity. Twenty-four ddY mice...

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Main Authors: Mahardian Rahmadi, Chia-Wei Phan, Santhra Segaran Balan, Athallah Syauqi Zumarthana, Ellyna Majesty Ayundari, Ismailia Wienda Yasmin Pratama Putri, Salsabila, Ahmad Dzulfikri Nurhan
Format: Article
Language:English
Published: Pensoft Publishers 2025-08-01
Series:Pharmacia
Online Access:https://pharmacia.pensoft.net/article/159024/download/pdf/
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author Mahardian Rahmadi
Chia-Wei Phan
Santhra Segaran Balan
Athallah Syauqi Zumarthana
Ellyna Majesty Ayundari
Ismailia Wienda Yasmin Pratama Putri
Salsabila
Ahmad Dzulfikri Nurhan
author_facet Mahardian Rahmadi
Chia-Wei Phan
Santhra Segaran Balan
Athallah Syauqi Zumarthana
Ellyna Majesty Ayundari
Ismailia Wienda Yasmin Pratama Putri
Salsabila
Ahmad Dzulfikri Nurhan
author_sort Mahardian Rahmadi
collection DOAJ
description Metabolic dysfunction-associated fatty liver disease (MAFLD) is characterized by intrahepatic fat accumulation in individuals without a history of excessive alcohol consumption. This study investigated the effects of epigallocatechin-3-gallate (EGCG) on reducing MAFLD severity. Twenty-four ddY mice were divided into three groups: normal feed, high-fat diet (HFD), and HFD + 25 mg/kg EGCG supplementation. All mice were provided with food and water ad libitum; the remaining feed and body weight were measured daily for 28 days. On the 15th day, the therapy group received EGCG intraperitoneally. At the end of the study, mice were sacrificed, and their livers were collected for histopathological examination and measurement of LKB1, CD86, and CD163 mRNA expression. The results indicated that LKB1 expression increased significantly following EGCG administration. Additionally, histopathological analysis revealed a positive impact of EGCG on hepatocyte repair. In conclusion, the 14-day EGCG treatment demonstrated promising results in improving liver health in mice with MAFLD and modulating LKB1 expression.
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institution Kabale University
issn 2603-557X
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publishDate 2025-08-01
publisher Pensoft Publishers
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series Pharmacia
spelling doaj-art-bf2d037a016d48ee835d805ce3ea5b0e2025-08-20T04:01:18ZengPensoft PublishersPharmacia2603-557X2025-08-017211110.3897/pharmacia.72.e159024159024Involvement of liver kinase B1 in the ameliorative action of epigallocatechin-3-gallate against MAFLD progression in miceMahardian Rahmadi0Chia-Wei Phan1Santhra Segaran Balan2Athallah Syauqi Zumarthana3Ellyna Majesty Ayundari4Ismailia Wienda Yasmin Pratama Putri5Salsabila6Ahmad Dzulfikri Nurhan7Universitas AirlanggaUniversity of MalayaManagement and Science UniversityUniversitas AirlanggaUniversitas AirlanggaUniversitas AirlanggaUniversitas AirlanggaTaipei Medical UniversityMetabolic dysfunction-associated fatty liver disease (MAFLD) is characterized by intrahepatic fat accumulation in individuals without a history of excessive alcohol consumption. This study investigated the effects of epigallocatechin-3-gallate (EGCG) on reducing MAFLD severity. Twenty-four ddY mice were divided into three groups: normal feed, high-fat diet (HFD), and HFD + 25 mg/kg EGCG supplementation. All mice were provided with food and water ad libitum; the remaining feed and body weight were measured daily for 28 days. On the 15th day, the therapy group received EGCG intraperitoneally. At the end of the study, mice were sacrificed, and their livers were collected for histopathological examination and measurement of LKB1, CD86, and CD163 mRNA expression. The results indicated that LKB1 expression increased significantly following EGCG administration. Additionally, histopathological analysis revealed a positive impact of EGCG on hepatocyte repair. In conclusion, the 14-day EGCG treatment demonstrated promising results in improving liver health in mice with MAFLD and modulating LKB1 expression.https://pharmacia.pensoft.net/article/159024/download/pdf/
spellingShingle Mahardian Rahmadi
Chia-Wei Phan
Santhra Segaran Balan
Athallah Syauqi Zumarthana
Ellyna Majesty Ayundari
Ismailia Wienda Yasmin Pratama Putri
Salsabila
Ahmad Dzulfikri Nurhan
Involvement of liver kinase B1 in the ameliorative action of epigallocatechin-3-gallate against MAFLD progression in mice
Pharmacia
title Involvement of liver kinase B1 in the ameliorative action of epigallocatechin-3-gallate against MAFLD progression in mice
title_full Involvement of liver kinase B1 in the ameliorative action of epigallocatechin-3-gallate against MAFLD progression in mice
title_fullStr Involvement of liver kinase B1 in the ameliorative action of epigallocatechin-3-gallate against MAFLD progression in mice
title_full_unstemmed Involvement of liver kinase B1 in the ameliorative action of epigallocatechin-3-gallate against MAFLD progression in mice
title_short Involvement of liver kinase B1 in the ameliorative action of epigallocatechin-3-gallate against MAFLD progression in mice
title_sort involvement of liver kinase b1 in the ameliorative action of epigallocatechin 3 gallate against mafld progression in mice
url https://pharmacia.pensoft.net/article/159024/download/pdf/
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