Nociceptive Neurons Differentially Express Fast and Slow T-Type Ca2+ Currents in Different Types of Diabetic Neuropathy
T-type Ca2+ channels are known as important participants of nociception and their remodeling contributes to diabetes-induced alterations of pain sensation. In this work we have established that about 30% of rat nonpeptidergic thermal C-type nociceptive (NTCN) neurons of segments L4–L6 express a slow...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2014/938235 |
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| author | Eugen V. Khomula Anya L. Borisyuk Viacheslav Y. Viatchenko-Karpinski Andrea Briede Pavel V. Belan Nana V. Voitenko |
| author_facet | Eugen V. Khomula Anya L. Borisyuk Viacheslav Y. Viatchenko-Karpinski Andrea Briede Pavel V. Belan Nana V. Voitenko |
| author_sort | Eugen V. Khomula |
| collection | DOAJ |
| description | T-type Ca2+ channels are known as important participants of nociception and their remodeling contributes to diabetes-induced alterations of pain sensation. In this work we have established that about 30% of rat nonpeptidergic thermal C-type nociceptive (NTCN) neurons of segments L4–L6 express a slow T-type Ca2+ current (T-current) while a fast T-current is expressed in the other 70% of these neurons. Streptozotocin-induced diabetes in young rats resulted in thermal hyperalgesia, hypoalgesia, or normalgesia 5-6 weeks after the induction. Our results show that NTCN neurons obtained from hyperalgesic animals do not express the slow T-current. Meanwhile, the fraction of neurons expressing the slow T-current did not significantly change in the hypo- and normalgesic diabetic groups. Moreover, the peak current density of fast T-current was significantly increased only in the neurons of hyperalgesic group. In contrast, the peak current density of slow T-current was significantly decreased in the hypo- and normalgesic groups. Experimental diabetes also resulted in a depolarizing shift of steady-state inactivation of fast T-current in the hyperalgesic group and slow T-current in the hypo- and normalgesic groups. We suggest that the observed changes may contribute to expression of different types of peripheral diabetic neuropathy occurring during the development of diabetes mellitus. |
| format | Article |
| id | doaj-art-bf17dd1a7e3347edbedc21dffbb5c8f0 |
| institution | DOAJ |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-bf17dd1a7e3347edbedc21dffbb5c8f02025-08-20T03:23:07ZengWileyNeural Plasticity2090-59041687-54432014-01-01201410.1155/2014/938235938235Nociceptive Neurons Differentially Express Fast and Slow T-Type Ca2+ Currents in Different Types of Diabetic NeuropathyEugen V. Khomula0Anya L. Borisyuk1Viacheslav Y. Viatchenko-Karpinski2Andrea Briede3Pavel V. Belan4Nana V. Voitenko5International Center of Molecular Physiology of National Academy of Sciences of Ukraine, 4 Bogomoletz Street., Kyiv 01024, UkraineState Key Laboratory of Molecular and Cellular Biology, Bogomoletz Institute of Physiology of National Academy of Sciences of Ukraine, 4 Bogomoletz Street, Kyiv 01024, UkraineState Key Laboratory of Molecular and Cellular Biology, Bogomoletz Institute of Physiology of National Academy of Sciences of Ukraine, 4 Bogomoletz Street, Kyiv 01024, UkraineState Key Laboratory of Molecular and Cellular Biology, Bogomoletz Institute of Physiology of National Academy of Sciences of Ukraine, 4 Bogomoletz Street, Kyiv 01024, UkraineInternational Center of Molecular Physiology of National Academy of Sciences of Ukraine, 4 Bogomoletz Street., Kyiv 01024, UkraineInternational Center of Molecular Physiology of National Academy of Sciences of Ukraine, 4 Bogomoletz Street., Kyiv 01024, UkraineT-type Ca2+ channels are known as important participants of nociception and their remodeling contributes to diabetes-induced alterations of pain sensation. In this work we have established that about 30% of rat nonpeptidergic thermal C-type nociceptive (NTCN) neurons of segments L4–L6 express a slow T-type Ca2+ current (T-current) while a fast T-current is expressed in the other 70% of these neurons. Streptozotocin-induced diabetes in young rats resulted in thermal hyperalgesia, hypoalgesia, or normalgesia 5-6 weeks after the induction. Our results show that NTCN neurons obtained from hyperalgesic animals do not express the slow T-current. Meanwhile, the fraction of neurons expressing the slow T-current did not significantly change in the hypo- and normalgesic diabetic groups. Moreover, the peak current density of fast T-current was significantly increased only in the neurons of hyperalgesic group. In contrast, the peak current density of slow T-current was significantly decreased in the hypo- and normalgesic groups. Experimental diabetes also resulted in a depolarizing shift of steady-state inactivation of fast T-current in the hyperalgesic group and slow T-current in the hypo- and normalgesic groups. We suggest that the observed changes may contribute to expression of different types of peripheral diabetic neuropathy occurring during the development of diabetes mellitus.http://dx.doi.org/10.1155/2014/938235 |
| spellingShingle | Eugen V. Khomula Anya L. Borisyuk Viacheslav Y. Viatchenko-Karpinski Andrea Briede Pavel V. Belan Nana V. Voitenko Nociceptive Neurons Differentially Express Fast and Slow T-Type Ca2+ Currents in Different Types of Diabetic Neuropathy Neural Plasticity |
| title | Nociceptive Neurons Differentially Express Fast and Slow T-Type Ca2+ Currents in Different Types of Diabetic Neuropathy |
| title_full | Nociceptive Neurons Differentially Express Fast and Slow T-Type Ca2+ Currents in Different Types of Diabetic Neuropathy |
| title_fullStr | Nociceptive Neurons Differentially Express Fast and Slow T-Type Ca2+ Currents in Different Types of Diabetic Neuropathy |
| title_full_unstemmed | Nociceptive Neurons Differentially Express Fast and Slow T-Type Ca2+ Currents in Different Types of Diabetic Neuropathy |
| title_short | Nociceptive Neurons Differentially Express Fast and Slow T-Type Ca2+ Currents in Different Types of Diabetic Neuropathy |
| title_sort | nociceptive neurons differentially express fast and slow t type ca2 currents in different types of diabetic neuropathy |
| url | http://dx.doi.org/10.1155/2014/938235 |
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