Metabolomics and transcriptomics analyses revealed overexpression of TaMGD enhances wheat plant heat stress resistance through multiple responses
Monogalactosyldiacylglycerol (MGDG), as the primary lipid component of thylakoid membranes, has a significant part in plant growth and stress response. The current study employed two transgenic wheat lines (MG1516 and MG1314) overexpressing the MGDG synthase gene (TaMGD) and wild-type cv ''...
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Elsevier
2025-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651325000740 |
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| author | Dongyun Ma Haizhou Hu Jianchao Feng Beiming Xu Chenyang Du Yang Yang Yingxin Xie Chenyang Wang |
| author_facet | Dongyun Ma Haizhou Hu Jianchao Feng Beiming Xu Chenyang Du Yang Yang Yingxin Xie Chenyang Wang |
| author_sort | Dongyun Ma |
| collection | DOAJ |
| description | Monogalactosyldiacylglycerol (MGDG), as the primary lipid component of thylakoid membranes, has a significant part in plant growth and stress response. The current study employed two transgenic wheat lines (MG1516 and MG1314) overexpressing the MGDG synthase gene (TaMGD) and wild-type cv ''JW1'' to explore the function of TaMGD in response to high temperature stress during the anthesis stage of wheat. Under high-temperature stress, the overexpressed wheat lines exhibited higher grain weight, increased antioxidant enzyme activity, and lower H2O2 and malondialdehyde contents in leaves. Transcriptomic analysis suggests that overexpression of TaMGD influenced multiple metabolic pathways in response to high-temperature stress, including carbon metabolism, amino acid metabolism, photosynthesis, and lipid-related metabolism. Overall, 146 differentially expressed metabolites (DEMs) were identified in MG1516 and wild-type (WT) under heat stress, with MG1516 exhibiting a higher number of upregulated metabolites, particularly glycolipids, organic acids, and organic oxygen compounds. Furthermore, lipid content and unsaturation analysis revealed that the overexpressing wheat line had a higher lipid content and greater saturation than WT under heat stress. Our findings demonstrate that overexpression of TaMGD in wheat affects multiple metabolic pathways, including photosynthesis, carbon, and amino acid metabolism, in reply to high-temperature stress through the modification of cell membrane lipid content, fatty acid unsaturation and other factors. |
| format | Article |
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| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
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| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-bf040afa18a5435fb2b7c8d330212ee82025-02-12T05:30:09ZengElsevierEcotoxicology and Environmental Safety0147-65132025-01-01290117738Metabolomics and transcriptomics analyses revealed overexpression of TaMGD enhances wheat plant heat stress resistance through multiple responsesDongyun Ma0Haizhou Hu1Jianchao Feng2Beiming Xu3Chenyang Du4Yang Yang5Yingxin Xie6Chenyang Wang7College of Agronomy, Henan Agricultural University, Zhengzhou 450046, China; National Wheat Technology Innovation Center, Henan Agricultural University, Zhengzhou 450046, China; State Key Laboratory of High-Efficiency Production of Wheat-Mazie Doubel Cropping, Zhengzhou 450046, China; Corresponding authors at: College of Agronomy, Henan Agricultural University, Zhengzhou 450046, China.College of Agronomy, Henan Agricultural University, Zhengzhou 450046, China; State Key Laboratory of High-Efficiency Production of Wheat-Mazie Doubel Cropping, Zhengzhou 450046, ChinaCollege of Agronomy, Henan Agricultural University, Zhengzhou 450046, China; State Key Laboratory of High-Efficiency Production of Wheat-Mazie Doubel Cropping, Zhengzhou 450046, ChinaCollege of Agronomy, Henan Agricultural University, Zhengzhou 450046, China; State Key Laboratory of High-Efficiency Production of Wheat-Mazie Doubel Cropping, Zhengzhou 450046, ChinaCollege of Agronomy, Henan Agricultural University, Zhengzhou 450046, China; State Key Laboratory of High-Efficiency Production of Wheat-Mazie Doubel Cropping, Zhengzhou 450046, ChinaCollege of Agronomy, Henan Agricultural University, Zhengzhou 450046, China; State Key Laboratory of High-Efficiency Production of Wheat-Mazie Doubel Cropping, Zhengzhou 450046, ChinaCollege of Agronomy, Henan Agricultural University, Zhengzhou 450046, China; State Key Laboratory of High-Efficiency Production of Wheat-Mazie Doubel Cropping, Zhengzhou 450046, ChinaCollege of Agronomy, Henan Agricultural University, Zhengzhou 450046, China; National Wheat Technology Innovation Center, Henan Agricultural University, Zhengzhou 450046, China; State Key Laboratory of High-Efficiency Production of Wheat-Mazie Doubel Cropping, Zhengzhou 450046, ChinaMonogalactosyldiacylglycerol (MGDG), as the primary lipid component of thylakoid membranes, has a significant part in plant growth and stress response. The current study employed two transgenic wheat lines (MG1516 and MG1314) overexpressing the MGDG synthase gene (TaMGD) and wild-type cv ''JW1'' to explore the function of TaMGD in response to high temperature stress during the anthesis stage of wheat. Under high-temperature stress, the overexpressed wheat lines exhibited higher grain weight, increased antioxidant enzyme activity, and lower H2O2 and malondialdehyde contents in leaves. Transcriptomic analysis suggests that overexpression of TaMGD influenced multiple metabolic pathways in response to high-temperature stress, including carbon metabolism, amino acid metabolism, photosynthesis, and lipid-related metabolism. Overall, 146 differentially expressed metabolites (DEMs) were identified in MG1516 and wild-type (WT) under heat stress, with MG1516 exhibiting a higher number of upregulated metabolites, particularly glycolipids, organic acids, and organic oxygen compounds. Furthermore, lipid content and unsaturation analysis revealed that the overexpressing wheat line had a higher lipid content and greater saturation than WT under heat stress. Our findings demonstrate that overexpression of TaMGD in wheat affects multiple metabolic pathways, including photosynthesis, carbon, and amino acid metabolism, in reply to high-temperature stress through the modification of cell membrane lipid content, fatty acid unsaturation and other factors.http://www.sciencedirect.com/science/article/pii/S0147651325000740High temperature stressMulti-omics joint analysisTransgenic wheat plantsPhysiological traits |
| spellingShingle | Dongyun Ma Haizhou Hu Jianchao Feng Beiming Xu Chenyang Du Yang Yang Yingxin Xie Chenyang Wang Metabolomics and transcriptomics analyses revealed overexpression of TaMGD enhances wheat plant heat stress resistance through multiple responses Ecotoxicology and Environmental Safety High temperature stress Multi-omics joint analysis Transgenic wheat plants Physiological traits |
| title | Metabolomics and transcriptomics analyses revealed overexpression of TaMGD enhances wheat plant heat stress resistance through multiple responses |
| title_full | Metabolomics and transcriptomics analyses revealed overexpression of TaMGD enhances wheat plant heat stress resistance through multiple responses |
| title_fullStr | Metabolomics and transcriptomics analyses revealed overexpression of TaMGD enhances wheat plant heat stress resistance through multiple responses |
| title_full_unstemmed | Metabolomics and transcriptomics analyses revealed overexpression of TaMGD enhances wheat plant heat stress resistance through multiple responses |
| title_short | Metabolomics and transcriptomics analyses revealed overexpression of TaMGD enhances wheat plant heat stress resistance through multiple responses |
| title_sort | metabolomics and transcriptomics analyses revealed overexpression of tamgd enhances wheat plant heat stress resistance through multiple responses |
| topic | High temperature stress Multi-omics joint analysis Transgenic wheat plants Physiological traits |
| url | http://www.sciencedirect.com/science/article/pii/S0147651325000740 |
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