Oxidative Stress and Complement Activation in Aqueous Cells and Vitreous from Patient with Vitreoretinal Diseases: Comparison Between Diabetic ERM and PDR
Background: Epiretinal membrane (ERM) and proliferative diabetic retinopathy (PDR) belong to the group of vitreoretinal diseases, characterized by impairments at both the retina and the vitreous. The non-diabetic and diabetic forms of ERM (no-dERM and dERM) as well as the PDR are caused by microvasc...
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MDPI AG
2025-07-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/14/7/841 |
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| author | Lucia Dinice Pamela Cosimi Graziana Esposito Fabio Scarinci Andrea Cacciamani Concetta Cafiero Luca Placentino Guido Ripandelli Alessandra Micera |
| author_facet | Lucia Dinice Pamela Cosimi Graziana Esposito Fabio Scarinci Andrea Cacciamani Concetta Cafiero Luca Placentino Guido Ripandelli Alessandra Micera |
| author_sort | Lucia Dinice |
| collection | DOAJ |
| description | Background: Epiretinal membrane (ERM) and proliferative diabetic retinopathy (PDR) belong to the group of vitreoretinal diseases, characterized by impairments at both the retina and the vitreous. The non-diabetic and diabetic forms of ERM (no-dERM and dERM) as well as the PDR are caused by microvascular disorder, which frequently occurs in association with inflammation and oxidative stress. To better characterize no-dERM, dERM, and PDR at the biomolecular level, we compared the expression of inflammatory, oxidative, lipidic peroxidation products, and complement receptors. Methods: Twenty-seven ocular fluids from patients who underwent phaco-vitrectomy were categorized as no-dERM (9, 4M/5F; 70.4 ± 6.4), dERM (6, 3M/3F; 73.2 ± 4.9), and PDR (6, 5M/1F; 63.7 ± 7.4). Six cataracts (CTR; 3M/3F; 77.7 ± 9.0) were collected for internal control of aqueous cells. Results: In aqueous cells, <i>p65NFkB</i>, <i>iNOS</i>, <i>Nox1/Nox4</i>, and <i>Nrf2</i> were significantly upregulated, and <i>Keap1</i> was downregulated in dERM compared with PDR and no-dERM. In aqueous cells, a significant upregulation for <i>C3aR1mRNA</i>, <i>C5aR1mRNA</i>, and <i>CFHmRNA</i> were observed in dERM. In vitreous, C3a, C5b9, and MDA levels were significantly increased in dERM compared with PDR and no-dERM. Conclusions: Inflammatory and ROS products, as well as <i>C3aR1/C5aR1</i> and soluble MDA, appear of great interest, as their expression in aqueous and vitreous might have potential prognostic and therapeutic values. |
| format | Article |
| id | doaj-art-bf01080ad48848d6b16a6f6c04c971b7 |
| institution | Kabale University |
| issn | 2076-3921 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
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| series | Antioxidants |
| spelling | doaj-art-bf01080ad48848d6b16a6f6c04c971b72025-08-20T03:58:30ZengMDPI AGAntioxidants2076-39212025-07-0114784110.3390/antiox14070841Oxidative Stress and Complement Activation in Aqueous Cells and Vitreous from Patient with Vitreoretinal Diseases: Comparison Between Diabetic ERM and PDRLucia Dinice0Pamela Cosimi1Graziana Esposito2Fabio Scarinci3Andrea Cacciamani4Concetta Cafiero5Luca Placentino6Guido Ripandelli7Alessandra Micera8Research and Development Laboratory for Biochemical, Molecular and Cellular Applications in Ophthalmological Science, IRCCS—Fondazione Bietti, 00184 Rome, ItalySurgical Retina Research Unit, IRCCS—Fondazione Bietti, 00184 Rome, ItalyResearch and Development Laboratory for Biochemical, Molecular and Cellular Applications in Ophthalmological Science, IRCCS—Fondazione Bietti, 00184 Rome, ItalySurgical Retina Research Unit, IRCCS—Fondazione Bietti, 00184 Rome, ItalySurgical Retina Research Unit, IRCCS—Fondazione Bietti, 00184 Rome, ItalyAnatomic Pathology Unit, Fabrizio Spaziani Hospital, 03100 Frosinone, ItalySurgical Retina Research Unit, IRCCS—Fondazione Bietti, 00184 Rome, ItalySurgical Retina Research Unit, IRCCS—Fondazione Bietti, 00184 Rome, ItalyResearch and Development Laboratory for Biochemical, Molecular and Cellular Applications in Ophthalmological Science, IRCCS—Fondazione Bietti, 00184 Rome, ItalyBackground: Epiretinal membrane (ERM) and proliferative diabetic retinopathy (PDR) belong to the group of vitreoretinal diseases, characterized by impairments at both the retina and the vitreous. The non-diabetic and diabetic forms of ERM (no-dERM and dERM) as well as the PDR are caused by microvascular disorder, which frequently occurs in association with inflammation and oxidative stress. To better characterize no-dERM, dERM, and PDR at the biomolecular level, we compared the expression of inflammatory, oxidative, lipidic peroxidation products, and complement receptors. Methods: Twenty-seven ocular fluids from patients who underwent phaco-vitrectomy were categorized as no-dERM (9, 4M/5F; 70.4 ± 6.4), dERM (6, 3M/3F; 73.2 ± 4.9), and PDR (6, 5M/1F; 63.7 ± 7.4). Six cataracts (CTR; 3M/3F; 77.7 ± 9.0) were collected for internal control of aqueous cells. Results: In aqueous cells, <i>p65NFkB</i>, <i>iNOS</i>, <i>Nox1/Nox4</i>, and <i>Nrf2</i> were significantly upregulated, and <i>Keap1</i> was downregulated in dERM compared with PDR and no-dERM. In aqueous cells, a significant upregulation for <i>C3aR1mRNA</i>, <i>C5aR1mRNA</i>, and <i>CFHmRNA</i> were observed in dERM. In vitreous, C3a, C5b9, and MDA levels were significantly increased in dERM compared with PDR and no-dERM. Conclusions: Inflammatory and ROS products, as well as <i>C3aR1/C5aR1</i> and soluble MDA, appear of great interest, as their expression in aqueous and vitreous might have potential prognostic and therapeutic values.https://www.mdpi.com/2076-3921/14/7/841vitreoretinal diseasesepiretinal membranesdiabetesproliferative diabetic retinopathycomplement systemreactive oxygen species |
| spellingShingle | Lucia Dinice Pamela Cosimi Graziana Esposito Fabio Scarinci Andrea Cacciamani Concetta Cafiero Luca Placentino Guido Ripandelli Alessandra Micera Oxidative Stress and Complement Activation in Aqueous Cells and Vitreous from Patient with Vitreoretinal Diseases: Comparison Between Diabetic ERM and PDR Antioxidants vitreoretinal diseases epiretinal membranes diabetes proliferative diabetic retinopathy complement system reactive oxygen species |
| title | Oxidative Stress and Complement Activation in Aqueous Cells and Vitreous from Patient with Vitreoretinal Diseases: Comparison Between Diabetic ERM and PDR |
| title_full | Oxidative Stress and Complement Activation in Aqueous Cells and Vitreous from Patient with Vitreoretinal Diseases: Comparison Between Diabetic ERM and PDR |
| title_fullStr | Oxidative Stress and Complement Activation in Aqueous Cells and Vitreous from Patient with Vitreoretinal Diseases: Comparison Between Diabetic ERM and PDR |
| title_full_unstemmed | Oxidative Stress and Complement Activation in Aqueous Cells and Vitreous from Patient with Vitreoretinal Diseases: Comparison Between Diabetic ERM and PDR |
| title_short | Oxidative Stress and Complement Activation in Aqueous Cells and Vitreous from Patient with Vitreoretinal Diseases: Comparison Between Diabetic ERM and PDR |
| title_sort | oxidative stress and complement activation in aqueous cells and vitreous from patient with vitreoretinal diseases comparison between diabetic erm and pdr |
| topic | vitreoretinal diseases epiretinal membranes diabetes proliferative diabetic retinopathy complement system reactive oxygen species |
| url | https://www.mdpi.com/2076-3921/14/7/841 |
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