A Nanomechanical Investigation of Three Putative Anti-Erosion Agents: Remineralisation and Protection against Demineralisation

An increasing interest in dental erosion as a clinical and scientific phenomenon has led to concerted efforts to identify agents which might protect against erosion. In this study, nanoindentation was used to investigate inhibition of erosive enamel demineralisation over time scales with direct clin...

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Main Authors: Ahmed Z. Abdullah, Anthony J. Ireland, Jonathan R. Sandy, Michele E. Barbour
Format: Article
Language:English
Published: Wiley 2012-01-01
Series:International Journal of Dentistry
Online Access:http://dx.doi.org/10.1155/2012/768126
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author Ahmed Z. Abdullah
Anthony J. Ireland
Jonathan R. Sandy
Michele E. Barbour
author_facet Ahmed Z. Abdullah
Anthony J. Ireland
Jonathan R. Sandy
Michele E. Barbour
author_sort Ahmed Z. Abdullah
collection DOAJ
description An increasing interest in dental erosion as a clinical and scientific phenomenon has led to concerted efforts to identify agents which might protect against erosion. In this study, nanoindentation was used to investigate inhibition of erosive enamel demineralisation over time scales with direct clinical relevance. Nanohardness of polished human enamel specimens (n=8 per group) was measured at baseline (B), after demineralisation (D1: citric acid, 0.3% w/v, pH3.20, 20s), after treatment (T), and after a second demineralisation (D2: as above). Data were analysed using repeated measures ANOVA. All specimens exhibited a similar reduction in nanohardness B-D1 in the range 35.2–39.5%. The positive control solution (saturated hydroxyapatite solution) and 4500 mg/L fluoride as NaF significantly increased nanohardness D1-T by 19.9% and 24.1%, respectively, whereas 1400 mg/L fluoride as NaF, casein phosphopeptide-amorphous calcium phosphate mousse and negative control (deionised water) had no significant effect. Nanohardness at D2 was indistinguishable for all groups, with total reduction in nanohardness B-D2 of 31.6% (4500 mg/L fluoride), 35.2% (positive control), 39.9% (1400 mg/L fluoride), 42.4% (negative control), and 43.7% (CPP-ACP product). In summary, 4500 mg/L fluoride significantly increased the nanohardness of previously demineralised enamel and resulted in the smallest total reduction in nanohardness but there were few statistically significant differences among the groups.
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spelling doaj-art-befda8d0d2984c59b082f3701d5cecec2025-08-20T02:18:58ZengWileyInternational Journal of Dentistry1687-87281687-87362012-01-01201210.1155/2012/768126768126A Nanomechanical Investigation of Three Putative Anti-Erosion Agents: Remineralisation and Protection against DemineralisationAhmed Z. Abdullah0Anthony J. Ireland1Jonathan R. Sandy2Michele E. Barbour3Oral Nanoscience, School of Oral & Dental Sciences, University of Bristol, Bristol BS1 2LY, UKChild Dental Health, School of Oral & Dental Sciences, University of Bristol, Bristol BS1 2LY, UKChild Dental Health, School of Oral & Dental Sciences, University of Bristol, Bristol BS1 2LY, UKOral Nanoscience, School of Oral & Dental Sciences, University of Bristol, Bristol BS1 2LY, UKAn increasing interest in dental erosion as a clinical and scientific phenomenon has led to concerted efforts to identify agents which might protect against erosion. In this study, nanoindentation was used to investigate inhibition of erosive enamel demineralisation over time scales with direct clinical relevance. Nanohardness of polished human enamel specimens (n=8 per group) was measured at baseline (B), after demineralisation (D1: citric acid, 0.3% w/v, pH3.20, 20s), after treatment (T), and after a second demineralisation (D2: as above). Data were analysed using repeated measures ANOVA. All specimens exhibited a similar reduction in nanohardness B-D1 in the range 35.2–39.5%. The positive control solution (saturated hydroxyapatite solution) and 4500 mg/L fluoride as NaF significantly increased nanohardness D1-T by 19.9% and 24.1%, respectively, whereas 1400 mg/L fluoride as NaF, casein phosphopeptide-amorphous calcium phosphate mousse and negative control (deionised water) had no significant effect. Nanohardness at D2 was indistinguishable for all groups, with total reduction in nanohardness B-D2 of 31.6% (4500 mg/L fluoride), 35.2% (positive control), 39.9% (1400 mg/L fluoride), 42.4% (negative control), and 43.7% (CPP-ACP product). In summary, 4500 mg/L fluoride significantly increased the nanohardness of previously demineralised enamel and resulted in the smallest total reduction in nanohardness but there were few statistically significant differences among the groups.http://dx.doi.org/10.1155/2012/768126
spellingShingle Ahmed Z. Abdullah
Anthony J. Ireland
Jonathan R. Sandy
Michele E. Barbour
A Nanomechanical Investigation of Three Putative Anti-Erosion Agents: Remineralisation and Protection against Demineralisation
International Journal of Dentistry
title A Nanomechanical Investigation of Three Putative Anti-Erosion Agents: Remineralisation and Protection against Demineralisation
title_full A Nanomechanical Investigation of Three Putative Anti-Erosion Agents: Remineralisation and Protection against Demineralisation
title_fullStr A Nanomechanical Investigation of Three Putative Anti-Erosion Agents: Remineralisation and Protection against Demineralisation
title_full_unstemmed A Nanomechanical Investigation of Three Putative Anti-Erosion Agents: Remineralisation and Protection against Demineralisation
title_short A Nanomechanical Investigation of Three Putative Anti-Erosion Agents: Remineralisation and Protection against Demineralisation
title_sort nanomechanical investigation of three putative anti erosion agents remineralisation and protection against demineralisation
url http://dx.doi.org/10.1155/2012/768126
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