Renal Tubular Cells from Hibernating Squirrels are Protected against Cisplatin Induced Apoptosis
Hibernating 13-lined ground squirrels are characterized by tolerance of severe hypothermia and hypoperfusion during torpor, followed by periodic warm reperfusion during IBA, conditions which are lethal to nonhibernating mammals. The aim of the present study was to determine whether protection from a...
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| Format: | Article |
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Wiley
2020-01-01
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| Series: | International Journal of Nephrology |
| Online Access: | http://dx.doi.org/10.1155/2020/6313749 |
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| author | Swati Jain Alkesh Jani |
| author_facet | Swati Jain Alkesh Jani |
| author_sort | Swati Jain |
| collection | DOAJ |
| description | Hibernating 13-lined ground squirrels are characterized by tolerance of severe hypothermia and hypoperfusion during torpor, followed by periodic warm reperfusion during IBA, conditions which are lethal to nonhibernating mammals. The aim of the present study was to determine whether protection from apoptosis was specific to torpor arousal cycles during hibernation or will also apply to cisplatin treatment on squirrel renal tubular cells (RTECs) that were procured during hibernation. Squirrel and mouse RTECs were treated with cisplatin, a potent inducer of RTEC apoptosis. Squirrel RTECs subjected to cisplatin had significantly less apoptosis, no cleaved caspase-3, and increased XIAP, pAkt, and pBAD versus mouse RTECs. To determine whether XIAP and Akt1 are necessary for RTEC protection against cisplatin induced apoptotic cell death, gene expression of Akt1 or XIAP was silenced in squirrel RTECs. Squirrel RTECs deficient in Akt1 and XIAP had increased apoptosis and cleaved caspase-3 when treated with cisplatin. Our results thus demonstrates that 13-lined ground squirrel RTECs possess intrinsic intracellular mechanisms by which they protect themselves from apoptotic cell death. Cisplatin induced acute kidney injury (AKI) may be avoided in cancer patients by employing mechanisms used by squirrel RTECs to protect against cisplatin induced tubular cell apoptosis. |
| format | Article |
| id | doaj-art-befccd5b922341638efdf29eaf2d37f8 |
| institution | Kabale University |
| issn | 2090-214X 2090-2158 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Nephrology |
| spelling | doaj-art-befccd5b922341638efdf29eaf2d37f82025-02-03T01:04:07ZengWileyInternational Journal of Nephrology2090-214X2090-21582020-01-01202010.1155/2020/63137496313749Renal Tubular Cells from Hibernating Squirrels are Protected against Cisplatin Induced ApoptosisSwati Jain0Alkesh Jani1University of Colorado, Denver, CO, USAUniversity of Colorado, Denver, CO, USAHibernating 13-lined ground squirrels are characterized by tolerance of severe hypothermia and hypoperfusion during torpor, followed by periodic warm reperfusion during IBA, conditions which are lethal to nonhibernating mammals. The aim of the present study was to determine whether protection from apoptosis was specific to torpor arousal cycles during hibernation or will also apply to cisplatin treatment on squirrel renal tubular cells (RTECs) that were procured during hibernation. Squirrel and mouse RTECs were treated with cisplatin, a potent inducer of RTEC apoptosis. Squirrel RTECs subjected to cisplatin had significantly less apoptosis, no cleaved caspase-3, and increased XIAP, pAkt, and pBAD versus mouse RTECs. To determine whether XIAP and Akt1 are necessary for RTEC protection against cisplatin induced apoptotic cell death, gene expression of Akt1 or XIAP was silenced in squirrel RTECs. Squirrel RTECs deficient in Akt1 and XIAP had increased apoptosis and cleaved caspase-3 when treated with cisplatin. Our results thus demonstrates that 13-lined ground squirrel RTECs possess intrinsic intracellular mechanisms by which they protect themselves from apoptotic cell death. Cisplatin induced acute kidney injury (AKI) may be avoided in cancer patients by employing mechanisms used by squirrel RTECs to protect against cisplatin induced tubular cell apoptosis.http://dx.doi.org/10.1155/2020/6313749 |
| spellingShingle | Swati Jain Alkesh Jani Renal Tubular Cells from Hibernating Squirrels are Protected against Cisplatin Induced Apoptosis International Journal of Nephrology |
| title | Renal Tubular Cells from Hibernating Squirrels are Protected against Cisplatin Induced Apoptosis |
| title_full | Renal Tubular Cells from Hibernating Squirrels are Protected against Cisplatin Induced Apoptosis |
| title_fullStr | Renal Tubular Cells from Hibernating Squirrels are Protected against Cisplatin Induced Apoptosis |
| title_full_unstemmed | Renal Tubular Cells from Hibernating Squirrels are Protected against Cisplatin Induced Apoptosis |
| title_short | Renal Tubular Cells from Hibernating Squirrels are Protected against Cisplatin Induced Apoptosis |
| title_sort | renal tubular cells from hibernating squirrels are protected against cisplatin induced apoptosis |
| url | http://dx.doi.org/10.1155/2020/6313749 |
| work_keys_str_mv | AT swatijain renaltubularcellsfromhibernatingsquirrelsareprotectedagainstcisplatininducedapoptosis AT alkeshjani renaltubularcellsfromhibernatingsquirrelsareprotectedagainstcisplatininducedapoptosis |