Camel Whey Protein Attenuates Acute Heat Stress-Induced Kidney Injury in Rats by Up-Regulating CYP2J Activity and Activating PI3K/AKT/eNOS to Inhibit Oxidative Stress
Camel whey protein (CWP) is a potent natural antioxidant, noted for its abundance of antioxidant amino acids. Despite its promising properties, the precise mechanisms underlying its effects remain inadequately explored. This study aims to investigate the impact of CWP on kidney damage induced by acu...
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MDPI AG
2024-10-01
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| Series: | Veterinary Sciences |
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| author | Xiaoxia Jing Donghua Du Bin Hou Deng Zhan Surong Hasi |
| author_facet | Xiaoxia Jing Donghua Du Bin Hou Deng Zhan Surong Hasi |
| author_sort | Xiaoxia Jing |
| collection | DOAJ |
| description | Camel whey protein (CWP) is a potent natural antioxidant, noted for its abundance of antioxidant amino acids. Despite its promising properties, the precise mechanisms underlying its effects remain inadequately explored. This study aims to investigate the impact of CWP on kidney damage induced by acute heat stress in rats, as well as to elucidate its mechanism of action. We assessed CWP’s influence on cytochrome P450 2J (CYP2J) activity during heat stress, measured oxidative stress levels, and evaluated renal injury using CYP2J knockout rats. Our findings indicate that acute heat stress reduces CYP2J expression, while CWP administration restores CYP2J activity and enhances PI3K/Akt signaling. However, CWP did not mitigate oxidative stress-induced kidney damage in CYP2J<sup>−/−</sup> rats, suggesting the necessity of CYP2J for its protective effects. |
| format | Article |
| id | doaj-art-bef4dff37bcb4ab98c73a42a8e1d4aa2 |
| institution | OA Journals |
| issn | 2306-7381 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
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| series | Veterinary Sciences |
| spelling | doaj-art-bef4dff37bcb4ab98c73a42a8e1d4aa22025-08-20T02:04:44ZengMDPI AGVeterinary Sciences2306-73812024-10-01111152410.3390/vetsci11110524Camel Whey Protein Attenuates Acute Heat Stress-Induced Kidney Injury in Rats by Up-Regulating CYP2J Activity and Activating PI3K/AKT/eNOS to Inhibit Oxidative StressXiaoxia Jing0Donghua Du1Bin Hou2Deng Zhan3Surong Hasi4Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, ChinaDepartment of Veterinary Medicine, College of Animal Science and Technology, Hebei North University, Zhangjiakou 075131, ChinaOrdos Vocational College of Eco-Environment, Ordos 017010, ChinaKey Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, ChinaKey Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCamel whey protein (CWP) is a potent natural antioxidant, noted for its abundance of antioxidant amino acids. Despite its promising properties, the precise mechanisms underlying its effects remain inadequately explored. This study aims to investigate the impact of CWP on kidney damage induced by acute heat stress in rats, as well as to elucidate its mechanism of action. We assessed CWP’s influence on cytochrome P450 2J (CYP2J) activity during heat stress, measured oxidative stress levels, and evaluated renal injury using CYP2J knockout rats. Our findings indicate that acute heat stress reduces CYP2J expression, while CWP administration restores CYP2J activity and enhances PI3K/Akt signaling. However, CWP did not mitigate oxidative stress-induced kidney damage in CYP2J<sup>−/−</sup> rats, suggesting the necessity of CYP2J for its protective effects.https://www.mdpi.com/2306-7381/11/11/524camel whey proteinnatural antioxidantsoxidative stresskidney injury |
| spellingShingle | Xiaoxia Jing Donghua Du Bin Hou Deng Zhan Surong Hasi Camel Whey Protein Attenuates Acute Heat Stress-Induced Kidney Injury in Rats by Up-Regulating CYP2J Activity and Activating PI3K/AKT/eNOS to Inhibit Oxidative Stress Veterinary Sciences camel whey protein natural antioxidants oxidative stress kidney injury |
| title | Camel Whey Protein Attenuates Acute Heat Stress-Induced Kidney Injury in Rats by Up-Regulating CYP2J Activity and Activating PI3K/AKT/eNOS to Inhibit Oxidative Stress |
| title_full | Camel Whey Protein Attenuates Acute Heat Stress-Induced Kidney Injury in Rats by Up-Regulating CYP2J Activity and Activating PI3K/AKT/eNOS to Inhibit Oxidative Stress |
| title_fullStr | Camel Whey Protein Attenuates Acute Heat Stress-Induced Kidney Injury in Rats by Up-Regulating CYP2J Activity and Activating PI3K/AKT/eNOS to Inhibit Oxidative Stress |
| title_full_unstemmed | Camel Whey Protein Attenuates Acute Heat Stress-Induced Kidney Injury in Rats by Up-Regulating CYP2J Activity and Activating PI3K/AKT/eNOS to Inhibit Oxidative Stress |
| title_short | Camel Whey Protein Attenuates Acute Heat Stress-Induced Kidney Injury in Rats by Up-Regulating CYP2J Activity and Activating PI3K/AKT/eNOS to Inhibit Oxidative Stress |
| title_sort | camel whey protein attenuates acute heat stress induced kidney injury in rats by up regulating cyp2j activity and activating pi3k akt enos to inhibit oxidative stress |
| topic | camel whey protein natural antioxidants oxidative stress kidney injury |
| url | https://www.mdpi.com/2306-7381/11/11/524 |
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