Two-step production of anti-inflammatory soluble factor by Lactobacillus reuteri CRL 1098.
We have demonstrated previously that a soluble factor (LrS) produced by Lactobacillus (L.) reuteri CRL 1098 modulates the inflammatory response triggered by lipopolysaccharide. In this study, the production of LrS by L. reuteri CRL 1098 was realized through two steps: i) bacterial biomass production...
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| Language: | English |
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Public Library of Science (PLoS)
2018-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0200426&type=printable |
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| author | Milagros Griet Graciela Font de Valdez Carla L Gerez Ana V Rodríguez |
| author_facet | Milagros Griet Graciela Font de Valdez Carla L Gerez Ana V Rodríguez |
| author_sort | Milagros Griet |
| collection | DOAJ |
| description | We have demonstrated previously that a soluble factor (LrS) produced by Lactobacillus (L.) reuteri CRL 1098 modulates the inflammatory response triggered by lipopolysaccharide. In this study, the production of LrS by L. reuteri CRL 1098 was realized through two steps: i) bacterial biomass production, ii) LrS production, where the bacterial biomass was able to live but did not proliferate. Therefore, the simultaneous evaluation of the effect of different factors on the growth and LrS production was performed. Biomass production was found to be dependent mainly on culture medium, while LrS production with anti-inflammatory activity depended on culture conditions of the biomass such as pH, agitation and growth phase. The L. reuteri CRL 1098 biomass and LrS production in the optimized culture media designed for this work reduced the complete process cost by approximately 95%, respectively to laboratory scale cost. |
| format | Article |
| id | doaj-art-bef16f75edfa4d73ac30cff0c8718b8c |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-bef16f75edfa4d73ac30cff0c8718b8c2025-08-20T02:03:58ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01137e020042610.1371/journal.pone.0200426Two-step production of anti-inflammatory soluble factor by Lactobacillus reuteri CRL 1098.Milagros GrietGraciela Font de ValdezCarla L GerezAna V RodríguezWe have demonstrated previously that a soluble factor (LrS) produced by Lactobacillus (L.) reuteri CRL 1098 modulates the inflammatory response triggered by lipopolysaccharide. In this study, the production of LrS by L. reuteri CRL 1098 was realized through two steps: i) bacterial biomass production, ii) LrS production, where the bacterial biomass was able to live but did not proliferate. Therefore, the simultaneous evaluation of the effect of different factors on the growth and LrS production was performed. Biomass production was found to be dependent mainly on culture medium, while LrS production with anti-inflammatory activity depended on culture conditions of the biomass such as pH, agitation and growth phase. The L. reuteri CRL 1098 biomass and LrS production in the optimized culture media designed for this work reduced the complete process cost by approximately 95%, respectively to laboratory scale cost.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0200426&type=printable |
| spellingShingle | Milagros Griet Graciela Font de Valdez Carla L Gerez Ana V Rodríguez Two-step production of anti-inflammatory soluble factor by Lactobacillus reuteri CRL 1098. PLoS ONE |
| title | Two-step production of anti-inflammatory soluble factor by Lactobacillus reuteri CRL 1098. |
| title_full | Two-step production of anti-inflammatory soluble factor by Lactobacillus reuteri CRL 1098. |
| title_fullStr | Two-step production of anti-inflammatory soluble factor by Lactobacillus reuteri CRL 1098. |
| title_full_unstemmed | Two-step production of anti-inflammatory soluble factor by Lactobacillus reuteri CRL 1098. |
| title_short | Two-step production of anti-inflammatory soluble factor by Lactobacillus reuteri CRL 1098. |
| title_sort | two step production of anti inflammatory soluble factor by lactobacillus reuteri crl 1098 |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0200426&type=printable |
| work_keys_str_mv | AT milagrosgriet twostepproductionofantiinflammatorysolublefactorbylactobacillusreutericrl1098 AT gracielafontdevaldez twostepproductionofantiinflammatorysolublefactorbylactobacillusreutericrl1098 AT carlalgerez twostepproductionofantiinflammatorysolublefactorbylactobacillusreutericrl1098 AT anavrodriguez twostepproductionofantiinflammatorysolublefactorbylactobacillusreutericrl1098 |