Regeneration of an infected skin wound under conditions of experimental steroid hyperglycemia

Purpose. To evaluate the regeneration of an infected skin wound in experimental steroid hyperglycemia in rats.Materials and methods. Regeneration of an infected rat skin wound (n = 30) was evaluated on the background of steroid hyperglycemia. Depending on the type of wound surface treatment, all obs...

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Main Authors: L. A. Balykova, V. I. Inchina, T. V. Tarasova, D. A. Khaydar, L. M. Mosina, I. V. Saushev, A. V. Kuchuk, A. Mutvakel, I. V. Begoulov, R. S. Tarasov, P. V. Ageev, D. S. Ovchenkov
Format: Article
Language:Russian
Published: QUASAR, LLC 2023-03-01
Series:Исследования и практика в медицине
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Online Access:https://www.rpmj.ru/rpmj/article/view/854
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author L. A. Balykova
V. I. Inchina
T. V. Tarasova
D. A. Khaydar
L. M. Mosina
I. V. Saushev
A. V. Kuchuk
A. Mutvakel
I. V. Begoulov
R. S. Tarasov
P. V. Ageev
D. S. Ovchenkov
author_facet L. A. Balykova
V. I. Inchina
T. V. Tarasova
D. A. Khaydar
L. M. Mosina
I. V. Saushev
A. V. Kuchuk
A. Mutvakel
I. V. Begoulov
R. S. Tarasov
P. V. Ageev
D. S. Ovchenkov
author_sort L. A. Balykova
collection DOAJ
description Purpose. To evaluate the regeneration of an infected skin wound in experimental steroid hyperglycemia in rats.Materials and methods. Regeneration of an infected rat skin wound (n = 30) was evaluated on the background of steroid hyperglycemia. Depending on the type of wound surface treatment, all observed animals were divided into 3 groups: the first group (n = 10) was treated with an ointment containing Cymbopogon proximus per os administration; the second group (n = 10) was treated with an ointment containing sodium fusidate 2 %; the third group (n = 10, control) the wound was treated with vaseline. After five days, local changes in the wound area and the activity of its regeneration were evaluated. We also studied the blood changes: the composition of the shaped elements and the content of total protein, albumins, transaminase activity (ALT, AST), total cholesterol, urea and creatinine.Results. The use of Cymbopogon proximus orally by rats of the first group, with induced diabetes mellitus, contributes to a decrease in serum glucose levels by almost two times compared with the second and third groups. Ointment containing Cymbopogon proximus has immunomodulatory properties: it prevents the development of leukopenia, promotes the activation of agranular immunocytes. The use of Cymbopogon proximus extract has a positive effect on the rate of wound regeneration due to several factors: reduction of hyperemia and edema, stimulation of leukocyte activity, and as a consequence activation of regeneration. We did not find a significant difference in the rate of wound regeneration when treated with an ointment based on Cymbopogon proximus extract and an ointment containing sodium fusidate (p > 0.05).Conclusion. The regeneration activity of an infected wound in rats with steroid hyperglycemia does not significantly differ when treated with antimicrobial ointment and Cymbopogon proximus. The extract of Cymbopogon proximus has a double effect in vivo: it reduces glucose levels and promotes wound healing in the condition of hyperglycemia, which is promising in the treatment of complications in diabetes mellitus.
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spelling doaj-art-bef105cea0cd4b84a22fcd5985cffd932025-08-20T03:21:59ZrusQUASAR, LLCИсследования и практика в медицине2410-18932023-03-01101909910.17709/10.17709/2410-1893-2023-10-1-8483Regeneration of an infected skin wound under conditions of experimental steroid hyperglycemiaL. A. Balykova0V. I. Inchina1T. V. Tarasova2D. A. Khaydar3L. M. Mosina4I. V. Saushev5A. V. Kuchuk6A. Mutvakel7I. V. Begoulov8R. S. Tarasov9P. V. Ageev10D. S. Ovchenkov11Ogarev National Research Mordovian State UniversityOgarev National Research Mordovian State UniversityOgarev National Research Mordovian State UniversityPeoples' Friendship University of Russia (RUDN University)Ogarev National Research Mordovian State UniversityOgarev National Research Mordovian State UniversityPeoples' Friendship University of Russia (RUDN University)Ogarev National Research Mordovian State UniversityOgarev National Research Mordovian State UniversityOgarev National Research Mordovian State UniversityPeoples' Friendship University of Russia (RUDN University)Ogarev National Research Mordovian State UniversityPurpose. To evaluate the regeneration of an infected skin wound in experimental steroid hyperglycemia in rats.Materials and methods. Regeneration of an infected rat skin wound (n = 30) was evaluated on the background of steroid hyperglycemia. Depending on the type of wound surface treatment, all observed animals were divided into 3 groups: the first group (n = 10) was treated with an ointment containing Cymbopogon proximus per os administration; the second group (n = 10) was treated with an ointment containing sodium fusidate 2 %; the third group (n = 10, control) the wound was treated with vaseline. After five days, local changes in the wound area and the activity of its regeneration were evaluated. We also studied the blood changes: the composition of the shaped elements and the content of total protein, albumins, transaminase activity (ALT, AST), total cholesterol, urea and creatinine.Results. The use of Cymbopogon proximus orally by rats of the first group, with induced diabetes mellitus, contributes to a decrease in serum glucose levels by almost two times compared with the second and third groups. Ointment containing Cymbopogon proximus has immunomodulatory properties: it prevents the development of leukopenia, promotes the activation of agranular immunocytes. The use of Cymbopogon proximus extract has a positive effect on the rate of wound regeneration due to several factors: reduction of hyperemia and edema, stimulation of leukocyte activity, and as a consequence activation of regeneration. We did not find a significant difference in the rate of wound regeneration when treated with an ointment based on Cymbopogon proximus extract and an ointment containing sodium fusidate (p &gt; 0.05).Conclusion. The regeneration activity of an infected wound in rats with steroid hyperglycemia does not significantly differ when treated with antimicrobial ointment and Cymbopogon proximus. The extract of Cymbopogon proximus has a double effect in vivo: it reduces glucose levels and promotes wound healing in the condition of hyperglycemia, which is promising in the treatment of complications in diabetes mellitus.https://www.rpmj.ru/rpmj/article/view/854steroid hyperglycemiadiabetes mellitusdiabetic footpurulent woundsherbal therapy<i>cymbopogon proximus</i>regeneration
spellingShingle L. A. Balykova
V. I. Inchina
T. V. Tarasova
D. A. Khaydar
L. M. Mosina
I. V. Saushev
A. V. Kuchuk
A. Mutvakel
I. V. Begoulov
R. S. Tarasov
P. V. Ageev
D. S. Ovchenkov
Regeneration of an infected skin wound under conditions of experimental steroid hyperglycemia
Исследования и практика в медицине
steroid hyperglycemia
diabetes mellitus
diabetic foot
purulent wounds
herbal therapy
<i>cymbopogon proximus</i>
regeneration
title Regeneration of an infected skin wound under conditions of experimental steroid hyperglycemia
title_full Regeneration of an infected skin wound under conditions of experimental steroid hyperglycemia
title_fullStr Regeneration of an infected skin wound under conditions of experimental steroid hyperglycemia
title_full_unstemmed Regeneration of an infected skin wound under conditions of experimental steroid hyperglycemia
title_short Regeneration of an infected skin wound under conditions of experimental steroid hyperglycemia
title_sort regeneration of an infected skin wound under conditions of experimental steroid hyperglycemia
topic steroid hyperglycemia
diabetes mellitus
diabetic foot
purulent wounds
herbal therapy
<i>cymbopogon proximus</i>
regeneration
url https://www.rpmj.ru/rpmj/article/view/854
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