Priestia megaterium cells are primed for surviving lethal doses of antibiotics and chemical stress
Abstract Antibiotic resistant infections kill millions worldwide yearly. However, a key factor in recurrent infections is antibiotic persisters. Persisters are not inherently antibiotic-resistant but can withstand antibiotic exposure by entering a non-dividing state. This tolerance often results in...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-02-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-025-07639-2 |
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| _version_ | 1823861755340652544 |
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| author | Manisha Guha Abhyudai Singh Nicholas C. Butzin |
| author_facet | Manisha Guha Abhyudai Singh Nicholas C. Butzin |
| author_sort | Manisha Guha |
| collection | DOAJ |
| description | Abstract Antibiotic resistant infections kill millions worldwide yearly. However, a key factor in recurrent infections is antibiotic persisters. Persisters are not inherently antibiotic-resistant but can withstand antibiotic exposure by entering a non-dividing state. This tolerance often results in prolonged antibiotic usage, increasing the likelihood of developing resistant strains. Here, we show the existence of “primed cells” in the Gram-positive bacterium Priestia megaterium, formerly known as Bacillus megaterium. These cells are pre-adapted to become persisters prior to lethal antibiotic stress. Remarkably, this prepared state is passed down through multiple generations via epigenetic memory, enhancing survival against antibiotics and other chemical stress. Previously, two distinct types of persisters were proposed: Type I and Type II, formed during stationary and log phases, respectively. However, our findings reveal that primed cells contribute to an increase in persisters during transition and stationary phases, with no evidence supporting distinct phenotypes between Type I and Type II persisters. |
| format | Article |
| id | doaj-art-bee6a9b6fc5248a480229736638ea959 |
| institution | Kabale University |
| issn | 2399-3642 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj-art-bee6a9b6fc5248a480229736638ea9592025-02-09T12:50:32ZengNature PortfolioCommunications Biology2399-36422025-02-018111410.1038/s42003-025-07639-2Priestia megaterium cells are primed for surviving lethal doses of antibiotics and chemical stressManisha Guha0Abhyudai Singh1Nicholas C. Butzin2Department of Biology and Microbiology, South Dakota State UniversityElectrical & Computer Engineering, University of DelawareDepartment of Biology and Microbiology, South Dakota State UniversityAbstract Antibiotic resistant infections kill millions worldwide yearly. However, a key factor in recurrent infections is antibiotic persisters. Persisters are not inherently antibiotic-resistant but can withstand antibiotic exposure by entering a non-dividing state. This tolerance often results in prolonged antibiotic usage, increasing the likelihood of developing resistant strains. Here, we show the existence of “primed cells” in the Gram-positive bacterium Priestia megaterium, formerly known as Bacillus megaterium. These cells are pre-adapted to become persisters prior to lethal antibiotic stress. Remarkably, this prepared state is passed down through multiple generations via epigenetic memory, enhancing survival against antibiotics and other chemical stress. Previously, two distinct types of persisters were proposed: Type I and Type II, formed during stationary and log phases, respectively. However, our findings reveal that primed cells contribute to an increase in persisters during transition and stationary phases, with no evidence supporting distinct phenotypes between Type I and Type II persisters.https://doi.org/10.1038/s42003-025-07639-2 |
| spellingShingle | Manisha Guha Abhyudai Singh Nicholas C. Butzin Priestia megaterium cells are primed for surviving lethal doses of antibiotics and chemical stress Communications Biology |
| title | Priestia megaterium cells are primed for surviving lethal doses of antibiotics and chemical stress |
| title_full | Priestia megaterium cells are primed for surviving lethal doses of antibiotics and chemical stress |
| title_fullStr | Priestia megaterium cells are primed for surviving lethal doses of antibiotics and chemical stress |
| title_full_unstemmed | Priestia megaterium cells are primed for surviving lethal doses of antibiotics and chemical stress |
| title_short | Priestia megaterium cells are primed for surviving lethal doses of antibiotics and chemical stress |
| title_sort | priestia megaterium cells are primed for surviving lethal doses of antibiotics and chemical stress |
| url | https://doi.org/10.1038/s42003-025-07639-2 |
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