A high-resolution haplotype collection uncovers somatic hybridization, recombination and intercontinental movement in oat crown rust.
The population structure and evolution of basidiomycetes like rust fungi are influenced by complex reproductive cycles and dikaryotic life stages where two independent nuclear haplotypes are present in the cell. The ability to alternate between asexual (clonal) and sexual reproduction increases the...
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Public Library of Science (PLoS)
2024-11-01
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| Series: | PLoS Genetics |
| Online Access: | https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1011493&type=printable |
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| author | Eva C Henningsen David Lewis Eric S Nazareno Hayley Mangelson Monica Sanchez Kyle Langford Yung-Fen Huang Brian J Steffenson Brendan Boesen Shahryar F Kianian Ivan Liachko Eric Stone Peter N Dodds Jana Sperschneider Melania Figueroa |
| author_facet | Eva C Henningsen David Lewis Eric S Nazareno Hayley Mangelson Monica Sanchez Kyle Langford Yung-Fen Huang Brian J Steffenson Brendan Boesen Shahryar F Kianian Ivan Liachko Eric Stone Peter N Dodds Jana Sperschneider Melania Figueroa |
| author_sort | Eva C Henningsen |
| collection | DOAJ |
| description | The population structure and evolution of basidiomycetes like rust fungi are influenced by complex reproductive cycles and dikaryotic life stages where two independent nuclear haplotypes are present in the cell. The ability to alternate between asexual (clonal) and sexual reproduction increases the evolutionary capacity in these species. Furthermore, exchange of intact nuclei (somatic hybridization) in rust fungi can allow for rapid generation of genetic variability outside of the sexual cycle. Puccinia coronata f. sp. avenae (Pca), the causal agent of oat crown rust, is a pathogen of global economic importance that is difficult to control due to rapid breakdown of host genetic resistance. The contribution of sexuality, clonality, and migration to virulence evolution varies across Pca populations. As such, the Pca pathosystem is ideal to address the role of mating type, recombination, mutation, and somatic hybridization in host adaptation. We expanded the existing resources for USA and South African populations by generating whole genome sequencing data of Taiwanese and Australian isolates. An atlas of 30 chromosome-level, fully-phased nuclear haplotypes from six USA isolates and nine Australian isolates was created to capture the genomic composition of key Pca lineages. At the haplotype level, we confirmed previous reports of genetic recombination in the USA population and additionally detected either sexual or cryptic recombination between Australian isolates, contrasting previous evaluations that suggested Pca populations in Australia to be purely clonal. We also identified somatic hybridization events in Pca that are not only associated with significant changes in fitness but also imply intercontinental migration of haplotypes, which provides further impetus for molecular monitoring of rust pathogen populations on a global scale. |
| format | Article |
| id | doaj-art-bee27104d16f445c9c9456324f4ff0d3 |
| institution | OA Journals |
| issn | 1553-7390 1553-7404 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj-art-bee27104d16f445c9c9456324f4ff0d32025-08-20T02:36:41ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042024-11-012011e101149310.1371/journal.pgen.1011493A high-resolution haplotype collection uncovers somatic hybridization, recombination and intercontinental movement in oat crown rust.Eva C HenningsenDavid LewisEric S NazarenoHayley MangelsonMonica SanchezKyle LangfordYung-Fen HuangBrian J SteffensonBrendan BoesenShahryar F KianianIvan LiachkoEric StonePeter N DoddsJana SperschneiderMelania FigueroaThe population structure and evolution of basidiomycetes like rust fungi are influenced by complex reproductive cycles and dikaryotic life stages where two independent nuclear haplotypes are present in the cell. The ability to alternate between asexual (clonal) and sexual reproduction increases the evolutionary capacity in these species. Furthermore, exchange of intact nuclei (somatic hybridization) in rust fungi can allow for rapid generation of genetic variability outside of the sexual cycle. Puccinia coronata f. sp. avenae (Pca), the causal agent of oat crown rust, is a pathogen of global economic importance that is difficult to control due to rapid breakdown of host genetic resistance. The contribution of sexuality, clonality, and migration to virulence evolution varies across Pca populations. As such, the Pca pathosystem is ideal to address the role of mating type, recombination, mutation, and somatic hybridization in host adaptation. We expanded the existing resources for USA and South African populations by generating whole genome sequencing data of Taiwanese and Australian isolates. An atlas of 30 chromosome-level, fully-phased nuclear haplotypes from six USA isolates and nine Australian isolates was created to capture the genomic composition of key Pca lineages. At the haplotype level, we confirmed previous reports of genetic recombination in the USA population and additionally detected either sexual or cryptic recombination between Australian isolates, contrasting previous evaluations that suggested Pca populations in Australia to be purely clonal. We also identified somatic hybridization events in Pca that are not only associated with significant changes in fitness but also imply intercontinental migration of haplotypes, which provides further impetus for molecular monitoring of rust pathogen populations on a global scale.https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1011493&type=printable |
| spellingShingle | Eva C Henningsen David Lewis Eric S Nazareno Hayley Mangelson Monica Sanchez Kyle Langford Yung-Fen Huang Brian J Steffenson Brendan Boesen Shahryar F Kianian Ivan Liachko Eric Stone Peter N Dodds Jana Sperschneider Melania Figueroa A high-resolution haplotype collection uncovers somatic hybridization, recombination and intercontinental movement in oat crown rust. PLoS Genetics |
| title | A high-resolution haplotype collection uncovers somatic hybridization, recombination and intercontinental movement in oat crown rust. |
| title_full | A high-resolution haplotype collection uncovers somatic hybridization, recombination and intercontinental movement in oat crown rust. |
| title_fullStr | A high-resolution haplotype collection uncovers somatic hybridization, recombination and intercontinental movement in oat crown rust. |
| title_full_unstemmed | A high-resolution haplotype collection uncovers somatic hybridization, recombination and intercontinental movement in oat crown rust. |
| title_short | A high-resolution haplotype collection uncovers somatic hybridization, recombination and intercontinental movement in oat crown rust. |
| title_sort | high resolution haplotype collection uncovers somatic hybridization recombination and intercontinental movement in oat crown rust |
| url | https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1011493&type=printable |
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