Antioxidant Activity of Baby Java Citrus Peel Extract Promotes Lung Tissue Repair in Mice Challenged by Lipopolysaccharides
Acute lung injury tends to be induced by infection or sepsis that disrupt alveolar and vascular permeability, neutrophil influx, and edema. Those impairments are worsened by the increase of oxidative stress along with hyperinflammation response. Oxidative stress in lung tissue could be indicated by...
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University of Brawijaya
2024-05-01
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| Series: | Journal of Tropical Life Science |
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| Online Access: | https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/3121 |
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| author | Farida Rachmawati Fikriya Novita Sari Sefihara Paramitha Sakti Muhammad Wisam Wira Sakti Sri Rahayu Aris Soewondo Muhaimin Rifa'i |
| author_facet | Farida Rachmawati Fikriya Novita Sari Sefihara Paramitha Sakti Muhammad Wisam Wira Sakti Sri Rahayu Aris Soewondo Muhaimin Rifa'i |
| author_sort | Farida Rachmawati |
| collection | DOAJ |
| description | Acute lung injury tends to be induced by infection or sepsis that disrupt alveolar and vascular permeability, neutrophil influx, and edema. Those impairments are worsened by the increase of oxidative stress along with hyperinflammation response. Oxidative stress in lung tissue could be indicated by malondialdehyde (MDA) and nuclear factor erythroid 2-related factor 2 (Nrf2) expression. This research aimed to evaluate the efficacy of Baby Java citrus peel extract (BJE) in suppressing oxidative stress and preventing lung injury in lipopolysaccharides (LPS)-induced mice. Twenty-five male BALB/c mice were divided into five groups consisting of untreated (N), LPS (A), and LPS-induced followed by treatment using BJE at various doses: 75 mg/kg BW (BJE-1), 105 mg/kg BW (BJE-2), and 150 mg/kg BW (BJE-3). Lungs were isolated for histopathological analysis also detection of MDA and Nrf2 using flow cytometry. BJE at the dose of 105 mg/kg BW could inhibit the alteration of lung histology following LPS challenge including alveolar and interstitial neutrophil infiltration, proteinaceous debris, and septal thickening. The same dose also showed good potency in suppressing MDA and Nrf2 levels as oxidative stress indicators. Our findings demonstrated protective effects of Baby Java citrus peel in acute lung injury and oxidative stress prevention after LPS exposure. |
| format | Article |
| id | doaj-art-bed7540f5aef4dc58c64c9f0c6471da0 |
| institution | DOAJ |
| issn | 2087-5517 2527-4376 |
| language | English |
| publishDate | 2024-05-01 |
| publisher | University of Brawijaya |
| record_format | Article |
| series | Journal of Tropical Life Science |
| spelling | doaj-art-bed7540f5aef4dc58c64c9f0c6471da02025-08-20T02:51:34ZengUniversity of BrawijayaJournal of Tropical Life Science2087-55172527-43762024-05-01142229 – 236229 – 23610.11594/jtls.14.02.033066Antioxidant Activity of Baby Java Citrus Peel Extract Promotes Lung Tissue Repair in Mice Challenged by LipopolysaccharidesFarida Rachmawati0https://orcid.org/0000-0002-2157-0729Fikriya Novita Sari1https://orcid.org/0000-0003-3381-5890Sefihara Paramitha Sakti2https://orcid.org/0009-0007-0415-2828Muhammad Wisam Wira Sakti3Sri Rahayu4https://orcid.org/0000-0003-1864-9775Aris Soewondo5https://orcid.org/0000-0001-5071-3074Muhaimin Rifa'i6https://orcid.org/0000-0001-5731-2951Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang 65145, IndonesiaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang 65145, IndonesiaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang 65145, IndonesiaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang 65145, IndonesiaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang 65145, IndonesiaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang 65145, IndonesiaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang 65145, IndonesiaAcute lung injury tends to be induced by infection or sepsis that disrupt alveolar and vascular permeability, neutrophil influx, and edema. Those impairments are worsened by the increase of oxidative stress along with hyperinflammation response. Oxidative stress in lung tissue could be indicated by malondialdehyde (MDA) and nuclear factor erythroid 2-related factor 2 (Nrf2) expression. This research aimed to evaluate the efficacy of Baby Java citrus peel extract (BJE) in suppressing oxidative stress and preventing lung injury in lipopolysaccharides (LPS)-induced mice. Twenty-five male BALB/c mice were divided into five groups consisting of untreated (N), LPS (A), and LPS-induced followed by treatment using BJE at various doses: 75 mg/kg BW (BJE-1), 105 mg/kg BW (BJE-2), and 150 mg/kg BW (BJE-3). Lungs were isolated for histopathological analysis also detection of MDA and Nrf2 using flow cytometry. BJE at the dose of 105 mg/kg BW could inhibit the alteration of lung histology following LPS challenge including alveolar and interstitial neutrophil infiltration, proteinaceous debris, and septal thickening. The same dose also showed good potency in suppressing MDA and Nrf2 levels as oxidative stress indicators. Our findings demonstrated protective effects of Baby Java citrus peel in acute lung injury and oxidative stress prevention after LPS exposure.https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/3121acute lung injuryantioxidantbaby java citrus peelmdanrf2 |
| spellingShingle | Farida Rachmawati Fikriya Novita Sari Sefihara Paramitha Sakti Muhammad Wisam Wira Sakti Sri Rahayu Aris Soewondo Muhaimin Rifa'i Antioxidant Activity of Baby Java Citrus Peel Extract Promotes Lung Tissue Repair in Mice Challenged by Lipopolysaccharides Journal of Tropical Life Science acute lung injury antioxidant baby java citrus peel mda nrf2 |
| title | Antioxidant Activity of Baby Java Citrus Peel Extract Promotes Lung Tissue Repair in Mice Challenged by Lipopolysaccharides |
| title_full | Antioxidant Activity of Baby Java Citrus Peel Extract Promotes Lung Tissue Repair in Mice Challenged by Lipopolysaccharides |
| title_fullStr | Antioxidant Activity of Baby Java Citrus Peel Extract Promotes Lung Tissue Repair in Mice Challenged by Lipopolysaccharides |
| title_full_unstemmed | Antioxidant Activity of Baby Java Citrus Peel Extract Promotes Lung Tissue Repair in Mice Challenged by Lipopolysaccharides |
| title_short | Antioxidant Activity of Baby Java Citrus Peel Extract Promotes Lung Tissue Repair in Mice Challenged by Lipopolysaccharides |
| title_sort | antioxidant activity of baby java citrus peel extract promotes lung tissue repair in mice challenged by lipopolysaccharides |
| topic | acute lung injury antioxidant baby java citrus peel mda nrf2 |
| url | https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/3121 |
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