The Influence of Cochlear Implant-Based Electric Stimulation on the Electrophysiological Characteristics of Cultured Spiral Ganglion Neurons
Background. Cochlear implant-based electrical stimulation may be an important reason to induce the residual hearing loss after cochlear implantation. In our previous study, we found that charge-balanced biphasic electrical stimulation inhibited the neurite growth of spiral ganglion neurons (SGNs) an...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-01-01
|
| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2020/3108490 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832550588523479040 |
|---|---|
| author | Na Shen Lei Zhou Bin Lai Shufeng Li |
| author_facet | Na Shen Lei Zhou Bin Lai Shufeng Li |
| author_sort | Na Shen |
| collection | DOAJ |
| description | Background. Cochlear implant-based electrical stimulation may be an important reason to induce the residual hearing loss after cochlear implantation. In our previous study, we found that charge-balanced biphasic electrical stimulation inhibited the neurite growth of spiral ganglion neurons (SGNs) and decreased Schwann cell density in vitro. In this study, we want to know whether cochlear implant-based electrical stimulation can induce the change of electrical activity in cultured SGNs. Methods. Spiral ganglion neuron electrical stimulation in vitro model is established using the devices delivering cochlear implant-based electrical stimulation. After 48 h treatment by 50 μA or 100 μA electrical stimulation, the action potential (AP) and voltage depended calcium current (ICa) of SGNs are recorded using whole-cell electrophysiological method. Results. The results show that the ICa of SGNs is decreased significantly in 50 μA and 100 μA electrical stimulation groups. The reversal potential of ICa is nearly +80 mV in control SGN, but the reversal potential decreases to +50 mV in 50 μA and 100 μA electrical stimulation groups. Interestingly, the AP amplitude, the AP latency, and the AP duration of SGNs have no statistically significant differences in all three groups. Conclusion. Our study suggests cochlear implant-based electrical stimulation only significantly inhibit the ICa of cultured SGNs but has no effect on the firing of AP, and the relation of ICa inhibition and SGN damage induced by electrical stimulation and its mechanism needs to be further studied. |
| format | Article |
| id | doaj-art-bed3914fdd2849dfa3d3442602617710 |
| institution | Kabale University |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-bed3914fdd2849dfa3d34426026177102025-02-03T06:06:29ZengWileyNeural Plasticity2090-59041687-54432020-01-01202010.1155/2020/31084903108490The Influence of Cochlear Implant-Based Electric Stimulation on the Electrophysiological Characteristics of Cultured Spiral Ganglion NeuronsNa Shen0Lei Zhou1Bin Lai2Shufeng Li3Department of Otolaryngology, Zhongshan Hospital, Fudan University, Shanghai, ChinaDepartment of Otolaryngology, Zhongshan Hospital, Fudan University, Shanghai, ChinaState Key Laboratory of Medical Neurobiology, Institutes of Brain Science, Fudan University, Shanghai 200032, ChinaENT Institute and Department of Otolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, ChinaBackground. Cochlear implant-based electrical stimulation may be an important reason to induce the residual hearing loss after cochlear implantation. In our previous study, we found that charge-balanced biphasic electrical stimulation inhibited the neurite growth of spiral ganglion neurons (SGNs) and decreased Schwann cell density in vitro. In this study, we want to know whether cochlear implant-based electrical stimulation can induce the change of electrical activity in cultured SGNs. Methods. Spiral ganglion neuron electrical stimulation in vitro model is established using the devices delivering cochlear implant-based electrical stimulation. After 48 h treatment by 50 μA or 100 μA electrical stimulation, the action potential (AP) and voltage depended calcium current (ICa) of SGNs are recorded using whole-cell electrophysiological method. Results. The results show that the ICa of SGNs is decreased significantly in 50 μA and 100 μA electrical stimulation groups. The reversal potential of ICa is nearly +80 mV in control SGN, but the reversal potential decreases to +50 mV in 50 μA and 100 μA electrical stimulation groups. Interestingly, the AP amplitude, the AP latency, and the AP duration of SGNs have no statistically significant differences in all three groups. Conclusion. Our study suggests cochlear implant-based electrical stimulation only significantly inhibit the ICa of cultured SGNs but has no effect on the firing of AP, and the relation of ICa inhibition and SGN damage induced by electrical stimulation and its mechanism needs to be further studied.http://dx.doi.org/10.1155/2020/3108490 |
| spellingShingle | Na Shen Lei Zhou Bin Lai Shufeng Li The Influence of Cochlear Implant-Based Electric Stimulation on the Electrophysiological Characteristics of Cultured Spiral Ganglion Neurons Neural Plasticity |
| title | The Influence of Cochlear Implant-Based Electric Stimulation on the Electrophysiological Characteristics of Cultured Spiral Ganglion Neurons |
| title_full | The Influence of Cochlear Implant-Based Electric Stimulation on the Electrophysiological Characteristics of Cultured Spiral Ganglion Neurons |
| title_fullStr | The Influence of Cochlear Implant-Based Electric Stimulation on the Electrophysiological Characteristics of Cultured Spiral Ganglion Neurons |
| title_full_unstemmed | The Influence of Cochlear Implant-Based Electric Stimulation on the Electrophysiological Characteristics of Cultured Spiral Ganglion Neurons |
| title_short | The Influence of Cochlear Implant-Based Electric Stimulation on the Electrophysiological Characteristics of Cultured Spiral Ganglion Neurons |
| title_sort | influence of cochlear implant based electric stimulation on the electrophysiological characteristics of cultured spiral ganglion neurons |
| url | http://dx.doi.org/10.1155/2020/3108490 |
| work_keys_str_mv | AT nashen theinfluenceofcochlearimplantbasedelectricstimulationontheelectrophysiologicalcharacteristicsofculturedspiralganglionneurons AT leizhou theinfluenceofcochlearimplantbasedelectricstimulationontheelectrophysiologicalcharacteristicsofculturedspiralganglionneurons AT binlai theinfluenceofcochlearimplantbasedelectricstimulationontheelectrophysiologicalcharacteristicsofculturedspiralganglionneurons AT shufengli theinfluenceofcochlearimplantbasedelectricstimulationontheelectrophysiologicalcharacteristicsofculturedspiralganglionneurons AT nashen influenceofcochlearimplantbasedelectricstimulationontheelectrophysiologicalcharacteristicsofculturedspiralganglionneurons AT leizhou influenceofcochlearimplantbasedelectricstimulationontheelectrophysiologicalcharacteristicsofculturedspiralganglionneurons AT binlai influenceofcochlearimplantbasedelectricstimulationontheelectrophysiologicalcharacteristicsofculturedspiralganglionneurons AT shufengli influenceofcochlearimplantbasedelectricstimulationontheelectrophysiologicalcharacteristicsofculturedspiralganglionneurons |