Ensemble refinement of mismodeled cryo-EM RNA structures using all-atom simulations

Abstract The advent of single-particle cryogenic electron microscopy (cryo-EM) has enabled near-atomic resolution imaging of large macromolecules, enhancing functional insights. However, current cryo-EM refinement tools condense all single-particle images into a single structure, which can misrepres...

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Bibliographic Details
Main Authors: Elisa Posani, Pavel Janoš, Daniel Haack, Navtej Toor, Massimiliano Bonomi, Alessandra Magistrato, Giovanni Bussi
Format: Article
Language:English
Published: Nature Portfolio 2025-05-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-025-59769-0
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