Primary Mechanism Study of Panax notoginseng Flower (Herb) on Myocardial Infarction in Rats
Background. Panax notoginseng (Burk.) F. H. Chen is one of the most common herbs in China. Because of its good efficacy and little adverse reaction, Panax notoginseng has been used widely to treat cardiovascular diseases (CVDs). Objective. To investigate effects of Panax notoginseng flower (PN-F) on...
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Wiley
2019-01-01
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| Series: | Cardiology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2019/8723076 |
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| author | Xin Zhou Zhi-Cheng Li Pei-Pei Chen Rui-Fang Xie |
| author_facet | Xin Zhou Zhi-Cheng Li Pei-Pei Chen Rui-Fang Xie |
| author_sort | Xin Zhou |
| collection | DOAJ |
| description | Background. Panax notoginseng (Burk.) F. H. Chen is one of the most common herbs in China. Because of its good efficacy and little adverse reaction, Panax notoginseng has been used widely to treat cardiovascular diseases (CVDs). Objective. To investigate effects of Panax notoginseng flower (PN-F) on rats with myocardial infarction (MI). Methods. The proximal left anterior descending coronary artery in rats was ligated to induce acute myocardial infarction. Then, animals were randomly assigned to four experimental groups: MI control group, Betaloc control group (with Betaloc 10 mg/kg/d), FD500 (low-dose) group (Panax notoginseng flower decoction 500 mg/kg, n=10), and FD1000 (high-dose) group (Panax notoginseng flower decoction 1000 mg/kg, n=10). Panax notoginseng flower decoction or Betaloc was orally administrated for two to four weeks before and after operation. Sham-operated group was used as a normal untreated group, in which animals were treated with double distilled water, once daily. HE (hematoxylin and eosin) staining, immunofluorescent assay, TUNEL assay, quantitative real-time PCR, and western blot analysis were, respectively, performed to observe morphology, count mean minimal vessels, investigate apoptotic cells, and record gene (HIF-1, VEGFA, and KDR) and protein (Bcl-2 and Bax) expressions. Results. Two weeks after MI, PN-F significantly enhanced capillary density in the border area of MI, decreased infarct size, improved minimal vessels, suppressed cell apoptosis, and enhanced expressions of genes (HIF-1, VEGFA, and KDR) and proteins (Bcl-2 and Bax). Conclusions. PN-F demonstrated a potential herb to treat rats with myocardial infarction through promoting angiogenesis and inhibition of apoptosis in the infarct area. |
| format | Article |
| id | doaj-art-bec96248245c472ba4dc5e1314fff07a |
| institution | Kabale University |
| issn | 2090-8016 2090-0597 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cardiology Research and Practice |
| spelling | doaj-art-bec96248245c472ba4dc5e1314fff07a2025-02-03T06:04:56ZengWileyCardiology Research and Practice2090-80162090-05972019-01-01201910.1155/2019/87230768723076Primary Mechanism Study of Panax notoginseng Flower (Herb) on Myocardial Infarction in RatsXin Zhou0Zhi-Cheng Li1Pei-Pei Chen2Rui-Fang Xie3Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, ChinaShanghai Pu Dong Hospital, Fu Dan University, Shanghai 200433, ChinaLonghua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, ChinaLonghua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, ChinaBackground. Panax notoginseng (Burk.) F. H. Chen is one of the most common herbs in China. Because of its good efficacy and little adverse reaction, Panax notoginseng has been used widely to treat cardiovascular diseases (CVDs). Objective. To investigate effects of Panax notoginseng flower (PN-F) on rats with myocardial infarction (MI). Methods. The proximal left anterior descending coronary artery in rats was ligated to induce acute myocardial infarction. Then, animals were randomly assigned to four experimental groups: MI control group, Betaloc control group (with Betaloc 10 mg/kg/d), FD500 (low-dose) group (Panax notoginseng flower decoction 500 mg/kg, n=10), and FD1000 (high-dose) group (Panax notoginseng flower decoction 1000 mg/kg, n=10). Panax notoginseng flower decoction or Betaloc was orally administrated for two to four weeks before and after operation. Sham-operated group was used as a normal untreated group, in which animals were treated with double distilled water, once daily. HE (hematoxylin and eosin) staining, immunofluorescent assay, TUNEL assay, quantitative real-time PCR, and western blot analysis were, respectively, performed to observe morphology, count mean minimal vessels, investigate apoptotic cells, and record gene (HIF-1, VEGFA, and KDR) and protein (Bcl-2 and Bax) expressions. Results. Two weeks after MI, PN-F significantly enhanced capillary density in the border area of MI, decreased infarct size, improved minimal vessels, suppressed cell apoptosis, and enhanced expressions of genes (HIF-1, VEGFA, and KDR) and proteins (Bcl-2 and Bax). Conclusions. PN-F demonstrated a potential herb to treat rats with myocardial infarction through promoting angiogenesis and inhibition of apoptosis in the infarct area.http://dx.doi.org/10.1155/2019/8723076 |
| spellingShingle | Xin Zhou Zhi-Cheng Li Pei-Pei Chen Rui-Fang Xie Primary Mechanism Study of Panax notoginseng Flower (Herb) on Myocardial Infarction in Rats Cardiology Research and Practice |
| title | Primary Mechanism Study of Panax notoginseng Flower (Herb) on Myocardial Infarction in Rats |
| title_full | Primary Mechanism Study of Panax notoginseng Flower (Herb) on Myocardial Infarction in Rats |
| title_fullStr | Primary Mechanism Study of Panax notoginseng Flower (Herb) on Myocardial Infarction in Rats |
| title_full_unstemmed | Primary Mechanism Study of Panax notoginseng Flower (Herb) on Myocardial Infarction in Rats |
| title_short | Primary Mechanism Study of Panax notoginseng Flower (Herb) on Myocardial Infarction in Rats |
| title_sort | primary mechanism study of panax notoginseng flower herb on myocardial infarction in rats |
| url | http://dx.doi.org/10.1155/2019/8723076 |
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