The RNA export factor, Nxt1, is required for tissue specific transcriptional regulation.
The highly conserved, Nxf/Nxt (TAP/p15) RNA nuclear export pathway is important for export of most mRNAs from the nucleus, by interacting with mRNAs and promoting their passage through nuclear pores. Nxt1 is essential for viability; using a partial loss of function allele, we reveal a role for this...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-06-01
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| Series: | PLoS Genetics |
| Online Access: | https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1003526&type=printable |
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| author | Simona Caporilli Yachuan Yu Jianqiao Jiang Helen White-Cooper |
| author_facet | Simona Caporilli Yachuan Yu Jianqiao Jiang Helen White-Cooper |
| author_sort | Simona Caporilli |
| collection | DOAJ |
| description | The highly conserved, Nxf/Nxt (TAP/p15) RNA nuclear export pathway is important for export of most mRNAs from the nucleus, by interacting with mRNAs and promoting their passage through nuclear pores. Nxt1 is essential for viability; using a partial loss of function allele, we reveal a role for this gene in tissue specific transcription. We show that many Drosophila melanogaster testis-specific mRNAs require Nxt1 for their accumulation. The transcripts that require Nxt1 also depend on a testis-specific transcription complex, tMAC. We show that loss of Nxt1 leads to reduced transcription of tMAC targets. A reporter transcript from a tMAC-dependent promoter is under-expressed in Nxt1 mutants, however the same transcript accumulates in mutants if driven by a tMAC-independent promoter. Thus, in Drosophila primary spermatocytes, the transcription factor used to activate expression of a transcript, rather than the RNA sequence itself or the core transcription machinery, determines whether this expression requires Nxt1. We additionally find that transcripts from intron-less genes are more sensitive to loss of Nxt1 function than those from intron-containing genes and propose a mechanism in which transcript processing feeds back to increase activity of a tissue specific transcription complex. |
| format | Article |
| id | doaj-art-bebd35eaf0ab43d1bde539c009ec561f |
| institution | OA Journals |
| issn | 1553-7390 1553-7404 |
| language | English |
| publishDate | 2013-06-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj-art-bebd35eaf0ab43d1bde539c009ec561f2025-08-20T02:33:43ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042013-06-0196e100352610.1371/journal.pgen.1003526The RNA export factor, Nxt1, is required for tissue specific transcriptional regulation.Simona CaporilliYachuan YuJianqiao JiangHelen White-CooperThe highly conserved, Nxf/Nxt (TAP/p15) RNA nuclear export pathway is important for export of most mRNAs from the nucleus, by interacting with mRNAs and promoting their passage through nuclear pores. Nxt1 is essential for viability; using a partial loss of function allele, we reveal a role for this gene in tissue specific transcription. We show that many Drosophila melanogaster testis-specific mRNAs require Nxt1 for their accumulation. The transcripts that require Nxt1 also depend on a testis-specific transcription complex, tMAC. We show that loss of Nxt1 leads to reduced transcription of tMAC targets. A reporter transcript from a tMAC-dependent promoter is under-expressed in Nxt1 mutants, however the same transcript accumulates in mutants if driven by a tMAC-independent promoter. Thus, in Drosophila primary spermatocytes, the transcription factor used to activate expression of a transcript, rather than the RNA sequence itself or the core transcription machinery, determines whether this expression requires Nxt1. We additionally find that transcripts from intron-less genes are more sensitive to loss of Nxt1 function than those from intron-containing genes and propose a mechanism in which transcript processing feeds back to increase activity of a tissue specific transcription complex.https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1003526&type=printable |
| spellingShingle | Simona Caporilli Yachuan Yu Jianqiao Jiang Helen White-Cooper The RNA export factor, Nxt1, is required for tissue specific transcriptional regulation. PLoS Genetics |
| title | The RNA export factor, Nxt1, is required for tissue specific transcriptional regulation. |
| title_full | The RNA export factor, Nxt1, is required for tissue specific transcriptional regulation. |
| title_fullStr | The RNA export factor, Nxt1, is required for tissue specific transcriptional regulation. |
| title_full_unstemmed | The RNA export factor, Nxt1, is required for tissue specific transcriptional regulation. |
| title_short | The RNA export factor, Nxt1, is required for tissue specific transcriptional regulation. |
| title_sort | rna export factor nxt1 is required for tissue specific transcriptional regulation |
| url | https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1003526&type=printable |
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