Inhibitory Effect of Different Biopreservatives on Repeated Freeze-thaw Oxidation in Tibetan Sheep

The objective of this study was to investigate the inhibitory effects of chitosan (CHI), lysozyme (LYS), and lactic acid bacteria (LAB) on lipid and myofibrillar protein (MP) oxidation during the repeated freeze-thaw process of Tibetan sheep meat. Five freeze-thaw cycles were conducted after sprayin...

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Main Authors: Hongyan DING, Zhenzhen YUAN, Guangjin YAN, Ke GAO, Lijuan HAN, Shengnan SUN, Shengzhen HOU
Format: Article
Language:zho
Published: The editorial department of Science and Technology of Food Industry 2025-04-01
Series:Shipin gongye ke-ji
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Online Access:http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024050028
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author Hongyan DING
Zhenzhen YUAN
Guangjin YAN
Ke GAO
Lijuan HAN
Shengnan SUN
Shengzhen HOU
author_facet Hongyan DING
Zhenzhen YUAN
Guangjin YAN
Ke GAO
Lijuan HAN
Shengnan SUN
Shengzhen HOU
author_sort Hongyan DING
collection DOAJ
description The objective of this study was to investigate the inhibitory effects of chitosan (CHI), lysozyme (LYS), and lactic acid bacteria (LAB) on lipid and myofibrillar protein (MP) oxidation during the repeated freeze-thaw process of Tibetan sheep meat. Five freeze-thaw cycles were conducted after spraying biopreservatives on the longest muscle of Tibetan sheep, and then the indicators of peroxide value, thiobarbituric acid value, carbonyl content, sulfhydryl content, surface hydrophobicity, turbidity, solubility, and other sample characteristics during the freeze-thaw process were measured and analyzed. The results showed that the above three biopreservatives had good effects to reduce the degree of lipid and MP oxidation in Tibetan sheep during the freeze-thaw process. Among them, lactic acid bacteria had the best effect. Following the fifth freeze-thaw cycle, compared with the control group, the peroxide and thiobarbituric acid values of the lactic acid bacteria treated group were 2.111 mmol/kg and 0.0315 mg/100 g lower than the control group, respectively. The carbonyl content, surface hydrophobicity, and turbidity were 2.720 nmol/mg prot, 12.404 μg, and 0.025 lower than the control group, respectively. The sulfhydryl content and solubility were 0.025 nmol/g and 14.25% higher than the control group, respectively. In conclusion, lactic acid bacteria effectively delayed the oxidation of lipids and MP during the freeze-thaw cycle of Tibetan sheep.
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issn 1002-0306
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publishDate 2025-04-01
publisher The editorial department of Science and Technology of Food Industry
record_format Article
series Shipin gongye ke-ji
spelling doaj-art-bebab48399e047e29606f2e3ca8f410b2025-08-20T02:41:25ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062025-04-0146730130710.13386/j.issn1002-0306.20240500282024050028-7Inhibitory Effect of Different Biopreservatives on Repeated Freeze-thaw Oxidation in Tibetan SheepHongyan DING0Zhenzhen YUAN1Guangjin YAN2Ke GAO3Lijuan HAN4Shengnan SUN5Shengzhen HOU6College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, ChinaCollege of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, ChinaCollege of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, ChinaCollege of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, ChinaCollege of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, ChinaCollege of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, ChinaCollege of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, ChinaThe objective of this study was to investigate the inhibitory effects of chitosan (CHI), lysozyme (LYS), and lactic acid bacteria (LAB) on lipid and myofibrillar protein (MP) oxidation during the repeated freeze-thaw process of Tibetan sheep meat. Five freeze-thaw cycles were conducted after spraying biopreservatives on the longest muscle of Tibetan sheep, and then the indicators of peroxide value, thiobarbituric acid value, carbonyl content, sulfhydryl content, surface hydrophobicity, turbidity, solubility, and other sample characteristics during the freeze-thaw process were measured and analyzed. The results showed that the above three biopreservatives had good effects to reduce the degree of lipid and MP oxidation in Tibetan sheep during the freeze-thaw process. Among them, lactic acid bacteria had the best effect. Following the fifth freeze-thaw cycle, compared with the control group, the peroxide and thiobarbituric acid values of the lactic acid bacteria treated group were 2.111 mmol/kg and 0.0315 mg/100 g lower than the control group, respectively. The carbonyl content, surface hydrophobicity, and turbidity were 2.720 nmol/mg prot, 12.404 μg, and 0.025 lower than the control group, respectively. The sulfhydryl content and solubility were 0.025 nmol/g and 14.25% higher than the control group, respectively. In conclusion, lactic acid bacteria effectively delayed the oxidation of lipids and MP during the freeze-thaw cycle of Tibetan sheep.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024050028tibetan sheeplactic acid bacteriachitosanlysozymefreeze-thaw cyclefat oxidationmyofibrillar protein oxidationcorrelation
spellingShingle Hongyan DING
Zhenzhen YUAN
Guangjin YAN
Ke GAO
Lijuan HAN
Shengnan SUN
Shengzhen HOU
Inhibitory Effect of Different Biopreservatives on Repeated Freeze-thaw Oxidation in Tibetan Sheep
Shipin gongye ke-ji
tibetan sheep
lactic acid bacteria
chitosan
lysozyme
freeze-thaw cycle
fat oxidation
myofibrillar protein oxidation
correlation
title Inhibitory Effect of Different Biopreservatives on Repeated Freeze-thaw Oxidation in Tibetan Sheep
title_full Inhibitory Effect of Different Biopreservatives on Repeated Freeze-thaw Oxidation in Tibetan Sheep
title_fullStr Inhibitory Effect of Different Biopreservatives on Repeated Freeze-thaw Oxidation in Tibetan Sheep
title_full_unstemmed Inhibitory Effect of Different Biopreservatives on Repeated Freeze-thaw Oxidation in Tibetan Sheep
title_short Inhibitory Effect of Different Biopreservatives on Repeated Freeze-thaw Oxidation in Tibetan Sheep
title_sort inhibitory effect of different biopreservatives on repeated freeze thaw oxidation in tibetan sheep
topic tibetan sheep
lactic acid bacteria
chitosan
lysozyme
freeze-thaw cycle
fat oxidation
myofibrillar protein oxidation
correlation
url http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024050028
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