Wasp venom peptide improves the proapoptotic activity of alendronate sodium in A549 lung cancer cells.

Wasp venom peptide improves the proapoptotic activity of alendronate sodium in A549 lung cancer cells.

<h4>Background</h4>Lung cancer in men and women is considered the leading cause for cancer-related mortality worldwide. Anti-cancer peptides represent a potential untapped reservoir of effective cancer therapy.<h4>Methodology</h4>Box-Behnken response surface design was applie...

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Main Authors: Nabil A Alhakamy, Solomon Z Okbazghi, Mohamed A Alfaleh, Wesam H Abdulaal, Rana B Bakhaidar, Mohammed O Alselami, Majed Al Zahrani, Hani M Alqarni, Adel F Alghaith, Sultan Alshehri, Shaimaa M Badr-Eldin, Hibah M Aldawsari, Omar D Al-Hejaili, Bander M Aldhabi, Wael A Mahdi
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2022-01-01
Series:PLoS ONE
Online Access:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0264093&type=printable
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Summary:<h4>Background</h4>Lung cancer in men and women is considered the leading cause for cancer-related mortality worldwide. Anti-cancer peptides represent a potential untapped reservoir of effective cancer therapy.<h4>Methodology</h4>Box-Behnken response surface design was applied for formulating Alendronate sodium (ALS)-mastoparan peptide (MP) nanoconjugates using Design-Expert software. The optimization process aimed at minimizing the size of the prepared ALS-MP nanoconjugates. ALS-MP nanoconjugates' particle size, encapsulation efficiency and the release profile were determined. Cytotoxicity, cell cycle, annexin V staining and caspase 3 analyses on A549 cells were carried out for the optimized formula.<h4>Results</h4>The results revealed that the optimized formula was of 134.91±5.1 nm particle size. The novel ALS-MP demonstrated the lowest IC50 (1.3 ± 0.34 μM) in comparison to ALS-Raw (37.6 ± 1.79 μM). Thus, the results indicated that when optimized ALS-MP nanoconjugate was used, the IC50 of ALS was also reduced by half. Cell cycle analysis demonstrated a significantly higher percentage of cells in the G2-M phase following the treatment with optimized ALS-MP nanoconjugates.<h4>Conclusion</h4>The optimized ALS-MP formula had significantly improved the parameters related to the cytotoxic activity towards A549 cells, compared to control, MP and ALS-Raw.
ISSN:1932-6203

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