Molecular marker-based species identification of carnivorous freshwater catfish (Wallago attu) from river Chenab, Pakistan
DNA barcoding, one of several procedures for identification of organisms, is an effective way to identify fish species. DNA-based identification is reliable and accurate to characterize an organism at different stages of its life cycle. Several reference libraries were used to link species by adding...
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| Format: | Article |
| Language: | English |
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Society of Eminent Biological Scientists
2024-07-01
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| Series: | International Journal of Applied and Experimental Biology |
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| Online Access: | https://submissions.eminentscientists.com/index.php/IJAEB/article/view/79 |
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| author | Parsa Riaz Muhammad Naeem Shoaib Hassan Ammar Danyal Naeem Zara Naeem Muhammad Azam Sheikh |
| author_facet | Parsa Riaz Muhammad Naeem Shoaib Hassan Ammar Danyal Naeem Zara Naeem Muhammad Azam Sheikh |
| author_sort | Parsa Riaz |
| collection | DOAJ |
| description | DNA barcoding, one of several procedures for identification of organisms, is an effective way to identify fish species. DNA-based identification is reliable and accurate to characterize an organism at different stages of its life cycle. Several reference libraries were used to link species by adding data to report species. For the sequencing of undetermined species, a customized primer was used. An unknown specimen was identified using a cytochrome c oxidase 1 primer. In this study, cytochrome c oxidase subunit 1 was used to identify Wallago attu at the molecular level. After sequencing, the mitochondrial cytochrome c oxidase subunit 1 gene was fragmented into 380 base pair pieces. A molecular-based identification approach, which comprised a neighbor-joining tree to estimate divergence, was used in addition to morphological identification to identify and authenticate the species under investigation. The COI barcodes were morphologically identified and matched with reference sequences of anticipated species. The GenBank databases received species reports with the entry number MT476360. The study's findings suggested that DNA barcoding is a viable and successful method for fish identification and for establishing a reliable DNA barcode reference library for these species. |
| format | Article |
| id | doaj-art-beb05de5769f46b29e7db682566824ea |
| institution | Kabale University |
| issn | 2790-6523 2790-6531 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | Society of Eminent Biological Scientists |
| record_format | Article |
| series | International Journal of Applied and Experimental Biology |
| spelling | doaj-art-beb05de5769f46b29e7db682566824ea2025-08-20T03:53:42ZengSociety of Eminent Biological ScientistsInternational Journal of Applied and Experimental Biology2790-65232790-65312024-07-013210511110.56612/ijaaeb.v1i1.7980Molecular marker-based species identification of carnivorous freshwater catfish (Wallago attu) from river Chenab, PakistanParsa Riaz0Muhammad Naeem1Shoaib Hassan2Ammar Danyal Naeem3Zara Naeem4Muhammad Azam Sheikh5Institute of Zoology, Bahauddin Zakariya University Multan, PakistanInstitute of Zoology, Bahauddin Zakariya University Multan, PakistanInstitute of Zoology, Bahauddin Zakariya University Multan, PakistanInstitute of Zoology, Bahauddin Zakariya University Multan, PakistanInstitute of Zoology, Bahauddin Zakariya University Multan, PakistanDepartment of Zoology Division of Science & Technology University of Education Lahore, Punjab PakistanDNA barcoding, one of several procedures for identification of organisms, is an effective way to identify fish species. DNA-based identification is reliable and accurate to characterize an organism at different stages of its life cycle. Several reference libraries were used to link species by adding data to report species. For the sequencing of undetermined species, a customized primer was used. An unknown specimen was identified using a cytochrome c oxidase 1 primer. In this study, cytochrome c oxidase subunit 1 was used to identify Wallago attu at the molecular level. After sequencing, the mitochondrial cytochrome c oxidase subunit 1 gene was fragmented into 380 base pair pieces. A molecular-based identification approach, which comprised a neighbor-joining tree to estimate divergence, was used in addition to morphological identification to identify and authenticate the species under investigation. The COI barcodes were morphologically identified and matched with reference sequences of anticipated species. The GenBank databases received species reports with the entry number MT476360. The study's findings suggested that DNA barcoding is a viable and successful method for fish identification and for establishing a reliable DNA barcode reference library for these species.https://submissions.eminentscientists.com/index.php/IJAEB/article/view/79cytochrome c oxidase dna barcodingfishmolecular identificationmolecular markers |
| spellingShingle | Parsa Riaz Muhammad Naeem Shoaib Hassan Ammar Danyal Naeem Zara Naeem Muhammad Azam Sheikh Molecular marker-based species identification of carnivorous freshwater catfish (Wallago attu) from river Chenab, Pakistan International Journal of Applied and Experimental Biology cytochrome c oxidase dna barcoding fish molecular identification molecular markers |
| title | Molecular marker-based species identification of carnivorous freshwater catfish (Wallago attu) from river Chenab, Pakistan |
| title_full | Molecular marker-based species identification of carnivorous freshwater catfish (Wallago attu) from river Chenab, Pakistan |
| title_fullStr | Molecular marker-based species identification of carnivorous freshwater catfish (Wallago attu) from river Chenab, Pakistan |
| title_full_unstemmed | Molecular marker-based species identification of carnivorous freshwater catfish (Wallago attu) from river Chenab, Pakistan |
| title_short | Molecular marker-based species identification of carnivorous freshwater catfish (Wallago attu) from river Chenab, Pakistan |
| title_sort | molecular marker based species identification of carnivorous freshwater catfish wallago attu from river chenab pakistan |
| topic | cytochrome c oxidase dna barcoding fish molecular identification molecular markers |
| url | https://submissions.eminentscientists.com/index.php/IJAEB/article/view/79 |
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