N-Acetyl-seryl-aspartyl-lysyl-proline Alleviates Renal Fibrosis Induced by Unilateral Ureteric Obstruction in BALB/C Mice
To expand the armamentarium of treatment for chronic kidney disease (CKD), we explored the utility of boosting endogenously synthesized N-acetyl-seryl-aspartyl-lysyl-proline (Ac-SDKP), which is augmented by inhibition of the angiotensin converting enzyme. Male BALB/c mice underwent unilateral ureter...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2015/283123 |
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| author | Gary C. W. Chan Wai Han Yiu Hao Jia Wu Dickson W. L. Wong Miao Lin Xiao Ru Huang Hui Yao Lan Sydney C. W. Tang |
| author_facet | Gary C. W. Chan Wai Han Yiu Hao Jia Wu Dickson W. L. Wong Miao Lin Xiao Ru Huang Hui Yao Lan Sydney C. W. Tang |
| author_sort | Gary C. W. Chan |
| collection | DOAJ |
| description | To expand the armamentarium of treatment for chronic kidney disease (CKD), we explored the utility of boosting endogenously synthesized N-acetyl-seryl-aspartyl-lysyl-proline (Ac-SDKP), which is augmented by inhibition of the angiotensin converting enzyme. Male BALB/c mice underwent unilateral ureteral ligation (UUO) or sham operation and received exogenously administered Ac-SDKP delivered via a subcutaneous osmotic minipump or Captopril treatment by oral gavage. Seven days after UUO, there were significant reductions in the expression of both collagen 1 and collagen 3 in kidneys treated with Ac-SDKP or Captopril, and there was a trend towards reductions in collagen IV, α-SMA, and MCP-1 versus control. However, no significant attenuation of interstitial injury or macrophage infiltration was observed. These findings are in contrary to observations in other models and underscore the fact that a longer treatment time frame may be required to yield anti-inflammatory effects in BALB/c mice treated with Ac-SDKP compared to untreated mice. Finding an effective treatment regimen for CKD requires fine-tuning of pharmacologic protocols. |
| format | Article |
| id | doaj-art-beaf275db73d4efba7ef1f4d6cdb73de |
| institution | DOAJ |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-beaf275db73d4efba7ef1f4d6cdb73de2025-08-20T03:23:02ZengWileyMediators of Inflammation0962-93511466-18612015-01-01201510.1155/2015/283123283123N-Acetyl-seryl-aspartyl-lysyl-proline Alleviates Renal Fibrosis Induced by Unilateral Ureteric Obstruction in BALB/C MiceGary C. W. Chan0Wai Han Yiu1Hao Jia Wu2Dickson W. L. Wong3Miao Lin4Xiao Ru Huang5Hui Yao Lan6Sydney C. W. Tang7Division of Nephrology, Department of Medicine, The University of Hong Kong, Hong KongDivision of Nephrology, Department of Medicine, The University of Hong Kong, Hong KongDivision of Nephrology, Department of Medicine, The University of Hong Kong, Hong KongDivision of Nephrology, Department of Medicine, The University of Hong Kong, Hong KongDivision of Nephrology, Department of Medicine, The University of Hong Kong, Hong KongDepartment of Medicine and Therapeutics and Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Hong KongDepartment of Medicine and Therapeutics and Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Hong KongDivision of Nephrology, Department of Medicine, The University of Hong Kong, Hong KongTo expand the armamentarium of treatment for chronic kidney disease (CKD), we explored the utility of boosting endogenously synthesized N-acetyl-seryl-aspartyl-lysyl-proline (Ac-SDKP), which is augmented by inhibition of the angiotensin converting enzyme. Male BALB/c mice underwent unilateral ureteral ligation (UUO) or sham operation and received exogenously administered Ac-SDKP delivered via a subcutaneous osmotic minipump or Captopril treatment by oral gavage. Seven days after UUO, there were significant reductions in the expression of both collagen 1 and collagen 3 in kidneys treated with Ac-SDKP or Captopril, and there was a trend towards reductions in collagen IV, α-SMA, and MCP-1 versus control. However, no significant attenuation of interstitial injury or macrophage infiltration was observed. These findings are in contrary to observations in other models and underscore the fact that a longer treatment time frame may be required to yield anti-inflammatory effects in BALB/c mice treated with Ac-SDKP compared to untreated mice. Finding an effective treatment regimen for CKD requires fine-tuning of pharmacologic protocols.http://dx.doi.org/10.1155/2015/283123 |
| spellingShingle | Gary C. W. Chan Wai Han Yiu Hao Jia Wu Dickson W. L. Wong Miao Lin Xiao Ru Huang Hui Yao Lan Sydney C. W. Tang N-Acetyl-seryl-aspartyl-lysyl-proline Alleviates Renal Fibrosis Induced by Unilateral Ureteric Obstruction in BALB/C Mice Mediators of Inflammation |
| title | N-Acetyl-seryl-aspartyl-lysyl-proline Alleviates Renal Fibrosis Induced by Unilateral Ureteric Obstruction in BALB/C Mice |
| title_full | N-Acetyl-seryl-aspartyl-lysyl-proline Alleviates Renal Fibrosis Induced by Unilateral Ureteric Obstruction in BALB/C Mice |
| title_fullStr | N-Acetyl-seryl-aspartyl-lysyl-proline Alleviates Renal Fibrosis Induced by Unilateral Ureteric Obstruction in BALB/C Mice |
| title_full_unstemmed | N-Acetyl-seryl-aspartyl-lysyl-proline Alleviates Renal Fibrosis Induced by Unilateral Ureteric Obstruction in BALB/C Mice |
| title_short | N-Acetyl-seryl-aspartyl-lysyl-proline Alleviates Renal Fibrosis Induced by Unilateral Ureteric Obstruction in BALB/C Mice |
| title_sort | n acetyl seryl aspartyl lysyl proline alleviates renal fibrosis induced by unilateral ureteric obstruction in balb c mice |
| url | http://dx.doi.org/10.1155/2015/283123 |
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