Genetic Diversity and Phylogenetic Analysis of Citrus tristeza virus Isolates from Turkey

The presence of Citrus tristeza virus (CTV) in Turkey has been known since the 1960s and the virus was detected in all citrus growing regions of the country. Even though serological and biological characteristics of CTV have been studied since the 1980s, molecular characteristics of CTV isolates hav...

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Main Authors: Gözde Erkiş-Güngör, Bayram Çevik
Format: Article
Language:English
Published: Wiley 2019-01-01
Series:Advances in Virology
Online Access:http://dx.doi.org/10.1155/2019/7163747
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author Gözde Erkiş-Güngör
Bayram Çevik
author_facet Gözde Erkiş-Güngör
Bayram Çevik
author_sort Gözde Erkiş-Güngör
collection DOAJ
description The presence of Citrus tristeza virus (CTV) in Turkey has been known since the 1960s and the virus was detected in all citrus growing regions of the country. Even though serological and biological characteristics of CTV have been studied since the 1980s, molecular characteristics of CTV isolates have not been studied to date in Turkey. In this study, molecular characteristics of 15 CTV isolates collected from different citrus growing regions of Turkey were determined by amplification, cloning, and sequencing of their major coat protein (CP) genes. The sequence analysis showed that the CP genes were highly conserved among Turkish isolates. However, isolates from different regions showed more genetic variation than isolates from the same region. Turkish isolates were clustered into three phylogenetic groups showing no association with geographical origins, host, or symptoms induced in indicator plants. Phylogenetic analysis of Turkish isolates with isolates from different citrus growing regions of the world including well-characterized type isolates of previously established strain specific groups revealed that some Turkish isolates were closely related to severe quick decline or stem pitting isolates. The results demonstrated that although CTV isolates from Turkey are considered biologically mild, majority of them contain severe components potentially causing quick decline or stem pitting.
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spelling doaj-art-beae011660cd48fe80151a7b481b556f2025-02-03T01:31:19ZengWileyAdvances in Virology1687-86391687-86472019-01-01201910.1155/2019/71637477163747Genetic Diversity and Phylogenetic Analysis of Citrus tristeza virus Isolates from TurkeyGözde Erkiş-Güngör0Bayram Çevik1Applied Sciences University of Isparta, Faculty of Agricultural Sciences and Technologies, Department of Plant Protection, 32260 Isparta, TurkeyApplied Sciences University of Isparta, Faculty of Agricultural Sciences and Technologies, Department of Plant Protection, 32260 Isparta, TurkeyThe presence of Citrus tristeza virus (CTV) in Turkey has been known since the 1960s and the virus was detected in all citrus growing regions of the country. Even though serological and biological characteristics of CTV have been studied since the 1980s, molecular characteristics of CTV isolates have not been studied to date in Turkey. In this study, molecular characteristics of 15 CTV isolates collected from different citrus growing regions of Turkey were determined by amplification, cloning, and sequencing of their major coat protein (CP) genes. The sequence analysis showed that the CP genes were highly conserved among Turkish isolates. However, isolates from different regions showed more genetic variation than isolates from the same region. Turkish isolates were clustered into three phylogenetic groups showing no association with geographical origins, host, or symptoms induced in indicator plants. Phylogenetic analysis of Turkish isolates with isolates from different citrus growing regions of the world including well-characterized type isolates of previously established strain specific groups revealed that some Turkish isolates were closely related to severe quick decline or stem pitting isolates. The results demonstrated that although CTV isolates from Turkey are considered biologically mild, majority of them contain severe components potentially causing quick decline or stem pitting.http://dx.doi.org/10.1155/2019/7163747
spellingShingle Gözde Erkiş-Güngör
Bayram Çevik
Genetic Diversity and Phylogenetic Analysis of Citrus tristeza virus Isolates from Turkey
Advances in Virology
title Genetic Diversity and Phylogenetic Analysis of Citrus tristeza virus Isolates from Turkey
title_full Genetic Diversity and Phylogenetic Analysis of Citrus tristeza virus Isolates from Turkey
title_fullStr Genetic Diversity and Phylogenetic Analysis of Citrus tristeza virus Isolates from Turkey
title_full_unstemmed Genetic Diversity and Phylogenetic Analysis of Citrus tristeza virus Isolates from Turkey
title_short Genetic Diversity and Phylogenetic Analysis of Citrus tristeza virus Isolates from Turkey
title_sort genetic diversity and phylogenetic analysis of citrus tristeza virus isolates from turkey
url http://dx.doi.org/10.1155/2019/7163747
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AT bayramcevik geneticdiversityandphylogeneticanalysisofcitrustristezavirusisolatesfromturkey