Modified planar sensors for the separate detection of certain cephalosporin antibiotics

Modifi ed screen-printed potentiometric sensors sensitive to cephalosporin antibiotics – cefuroxime (Cefur), cefotaxime (Ceftx), cefazolin (Cef) have been developed. Tetradecylammonium associates with complex compounds silver (I) – cefuroxime (cefotaxime) have been used...

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Main Authors: Kulapina, Elena Grigorievna, Mursalov, Ruslan K., Kulapina, Olga Ivanovna
Format: Article
Language:English
Published: Saratov State University 2025-03-01
Series:Известия Саратовского университета. Новая серия: Серия Химия. Биология. Экология
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Online Access:https://ichbe.sgu.ru/sites/ichbe.sgu.ru/files/text-pdf/2025/04/himiya_2025_1-42-55.pdf
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author Kulapina, Elena Grigorievna
Mursalov, Ruslan K.
Kulapina, Olga Ivanovna
author_facet Kulapina, Elena Grigorievna
Mursalov, Ruslan K.
Kulapina, Olga Ivanovna
author_sort Kulapina, Elena Grigorievna
collection DOAJ
description Modifi ed screen-printed potentiometric sensors sensitive to cephalosporin antibiotics – cefuroxime (Cefur), cefotaxime (Ceftx), cefazolin (Cef) have been developed. Tetradecylammonium associates with complex compounds silver (I) – cefuroxime (cefotaxime) have been used as electroactive components. The role of magnetic nanoparticles Fe3O4 and cetylpyridinium chloride in improving the electroanalytical properties of sensors in solutions of the studied antibiotics is shown. Optimal ratios of modifi ers in carbon-containing inks (Fe3O4 : CPCh = 1 : 2.5) have been found. The main electroanalytical characteristics of the modifi ed sensors are determined. The introduction of a binary mixture of magnetic nanoparticles and cetylpyridinium chloride into carbon-containing inks leads to an improvement in the electroanalytical properties of planar sensors sensitive to cefuroxime, cefotaxime, cefazolin: at the same time, the detection limit of 1 · 10-6 (1 · 10-7 M) decreases, angular coeffi cients (55 ± 3 mV/pC) and linearity intervals of electrode functions (1 · 10-6 – 1 · 10-2 M), the response time is reduced – 26–30 seconds. The adsorption of surfactants at the interface ensures the stability of the suspension of nanoparticles and allows the concentration of analyte molecules. The possibility of separate determination of cephalosporin antibiotics in two- and three-component mixtures by projection methods of multidimensional data processing PLS-1 and PLS-2 is shown. The standard errors of calibration and prediction are estimated, on the basis of which the optimal number of latent variables for these methods is selected. It is established that all the studied models give high values of correlation coeffi cients and tangents of the slope of the “measured-predicted” dependencies close to one, which makes it possible to recommend these methods for practical use.
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issn 1816-9775
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publishDate 2025-03-01
publisher Saratov State University
record_format Article
series Известия Саратовского университета. Новая серия: Серия Химия. Биология. Экология
spelling doaj-art-bea6180ba3b04028a435ddc3cbdd35022025-08-24T15:01:50ZengSaratov State UniversityИзвестия Саратовского университета. Новая серия: Серия Химия. Биология. Экология1816-97752541-89712025-03-01251425510.18500/1816-9775-2025-25-1-42-55Modified planar sensors for the separate detection of certain cephalosporin antibioticsKulapina, Elena Grigorievna0Mursalov, Ruslan K.1Kulapina, Olga Ivanovna2Saratov State University, 83, Astrakhanskaya str., Saratov, 410012, RussiaSaratov State University, 83, Astrakhanskaya str., Saratov, 410012, RussiaSaratov State Medical University, 112, Bolshaya Kazachya Str., Saratov, 410010, RussiaModifi ed screen-printed potentiometric sensors sensitive to cephalosporin antibiotics – cefuroxime (Cefur), cefotaxime (Ceftx), cefazolin (Cef) have been developed. Tetradecylammonium associates with complex compounds silver (I) – cefuroxime (cefotaxime) have been used as electroactive components. The role of magnetic nanoparticles Fe3O4 and cetylpyridinium chloride in improving the electroanalytical properties of sensors in solutions of the studied antibiotics is shown. Optimal ratios of modifi ers in carbon-containing inks (Fe3O4 : CPCh = 1 : 2.5) have been found. The main electroanalytical characteristics of the modifi ed sensors are determined. The introduction of a binary mixture of magnetic nanoparticles and cetylpyridinium chloride into carbon-containing inks leads to an improvement in the electroanalytical properties of planar sensors sensitive to cefuroxime, cefotaxime, cefazolin: at the same time, the detection limit of 1 · 10-6 (1 · 10-7 M) decreases, angular coeffi cients (55 ± 3 mV/pC) and linearity intervals of electrode functions (1 · 10-6 – 1 · 10-2 M), the response time is reduced – 26–30 seconds. The adsorption of surfactants at the interface ensures the stability of the suspension of nanoparticles and allows the concentration of analyte molecules. The possibility of separate determination of cephalosporin antibiotics in two- and three-component mixtures by projection methods of multidimensional data processing PLS-1 and PLS-2 is shown. The standard errors of calibration and prediction are estimated, on the basis of which the optimal number of latent variables for these methods is selected. It is established that all the studied models give high values of correlation coeffi cients and tangents of the slope of the “measured-predicted” dependencies close to one, which makes it possible to recommend these methods for practical use.https://ichbe.sgu.ru/sites/ichbe.sgu.ru/files/text-pdf/2025/04/himiya_2025_1-42-55.pdfantibioticscefuroximecefotaximecefazolinpotentiometryprojection method on latent structures
spellingShingle Kulapina, Elena Grigorievna
Mursalov, Ruslan K.
Kulapina, Olga Ivanovna
Modified planar sensors for the separate detection of certain cephalosporin antibiotics
Известия Саратовского университета. Новая серия: Серия Химия. Биология. Экология
antibiotics
cefuroxime
cefotaxime
cefazolin
potentiometry
projection method on latent structures
title Modified planar sensors for the separate detection of certain cephalosporin antibiotics
title_full Modified planar sensors for the separate detection of certain cephalosporin antibiotics
title_fullStr Modified planar sensors for the separate detection of certain cephalosporin antibiotics
title_full_unstemmed Modified planar sensors for the separate detection of certain cephalosporin antibiotics
title_short Modified planar sensors for the separate detection of certain cephalosporin antibiotics
title_sort modified planar sensors for the separate detection of certain cephalosporin antibiotics
topic antibiotics
cefuroxime
cefotaxime
cefazolin
potentiometry
projection method on latent structures
url https://ichbe.sgu.ru/sites/ichbe.sgu.ru/files/text-pdf/2025/04/himiya_2025_1-42-55.pdf
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