Wound Healing, Cell Viability and Antimicrobial Potency of Mucus from Pangasianodon hypophthalmus
Acute and chronic wounds are the major cause of death according to World Health Organization (WHO), in which, antimicrobial resistance is considered to be a major plight. In this regard, our study is aimed at developing an antimicrobial agent using the mucus of Pangasianodon hypophthalmus against th...
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Journal of Pure and Applied Microbiology
2024-12-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/wound-healing-cell-viability-and-antimicrobial-potency-of-mucus-from-pangasianodon-hypophthalmus/ |
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| author | M. Gomathy Manikantan Pappuswamy |
| author_facet | M. Gomathy Manikantan Pappuswamy |
| author_sort | M. Gomathy |
| collection | DOAJ |
| description | Acute and chronic wounds are the major cause of death according to World Health Organization (WHO), in which, antimicrobial resistance is considered to be a major plight. In this regard, our study is aimed at developing an antimicrobial agent using the mucus of Pangasianodon hypophthalmus against the clinically resistant microbial pathogens and to evaluate the cell cytotoxicity and cell viability followed by an in vitro wound healing analysis. The evaluation of antimicrobial activity was performed through well diffusion method and micro dilution method. The cell cytotoxicity and cell viability were assessed using MTT assay. The cell migration and in vitro wound healing was performed using scratch assay. The acidic extracts of mucus showed antimicrobial activity against the eight different selected bacterial strains while the organic extract showed against seven bacterial strains. L929 showed a cell viability of 102.96% at a concentration of 75 µg/mL and did not show cell toxicity effect up to the concentration of 300 µg/mL. In the in vitro wound healing analysis, the cell migration rate was 99.27% in the treated cells while, the untreated showed only 94.68%. The current research work clearly shows that the mucus of P. hypophthalmus possesses antimicrobial activity and wound healing potency. Furthermore, gene expression analysis and in vivo trials have to be performed for a thorough understanding of the actual cellular mechanism of wound healing. |
| format | Article |
| id | doaj-art-bea356ef57dc446c9137a979d0b216de |
| institution | OA Journals |
| issn | 0973-7510 2581-690X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj-art-bea356ef57dc446c9137a979d0b216de2025-08-20T02:38:30ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2024-12-0118424112423https://doi.org/10.22207/JPAM.18.4.14Wound Healing, Cell Viability and Antimicrobial Potency of Mucus from Pangasianodon hypophthalmusM. Gomathyhttps://orcid.org/0000-0003-3051-6801Manikantan Pappuswamyhttps://orcid.org/0000-0002-6047-3702Acute and chronic wounds are the major cause of death according to World Health Organization (WHO), in which, antimicrobial resistance is considered to be a major plight. In this regard, our study is aimed at developing an antimicrobial agent using the mucus of Pangasianodon hypophthalmus against the clinically resistant microbial pathogens and to evaluate the cell cytotoxicity and cell viability followed by an in vitro wound healing analysis. The evaluation of antimicrobial activity was performed through well diffusion method and micro dilution method. The cell cytotoxicity and cell viability were assessed using MTT assay. The cell migration and in vitro wound healing was performed using scratch assay. The acidic extracts of mucus showed antimicrobial activity against the eight different selected bacterial strains while the organic extract showed against seven bacterial strains. L929 showed a cell viability of 102.96% at a concentration of 75 µg/mL and did not show cell toxicity effect up to the concentration of 300 µg/mL. In the in vitro wound healing analysis, the cell migration rate was 99.27% in the treated cells while, the untreated showed only 94.68%. The current research work clearly shows that the mucus of P. hypophthalmus possesses antimicrobial activity and wound healing potency. Furthermore, gene expression analysis and in vivo trials have to be performed for a thorough understanding of the actual cellular mechanism of wound healing.https://microbiologyjournal.org/wound-healing-cell-viability-and-antimicrobial-potency-of-mucus-from-pangasianodon-hypophthalmus/pangasianodon hypophthalmuscell cytotoxicitycell migrationmucus extractswound healing |
| spellingShingle | M. Gomathy Manikantan Pappuswamy Wound Healing, Cell Viability and Antimicrobial Potency of Mucus from Pangasianodon hypophthalmus Journal of Pure and Applied Microbiology pangasianodon hypophthalmus cell cytotoxicity cell migration mucus extracts wound healing |
| title | Wound Healing, Cell Viability and Antimicrobial Potency of Mucus from Pangasianodon hypophthalmus |
| title_full | Wound Healing, Cell Viability and Antimicrobial Potency of Mucus from Pangasianodon hypophthalmus |
| title_fullStr | Wound Healing, Cell Viability and Antimicrobial Potency of Mucus from Pangasianodon hypophthalmus |
| title_full_unstemmed | Wound Healing, Cell Viability and Antimicrobial Potency of Mucus from Pangasianodon hypophthalmus |
| title_short | Wound Healing, Cell Viability and Antimicrobial Potency of Mucus from Pangasianodon hypophthalmus |
| title_sort | wound healing cell viability and antimicrobial potency of mucus from pangasianodon hypophthalmus |
| topic | pangasianodon hypophthalmus cell cytotoxicity cell migration mucus extracts wound healing |
| url | https://microbiologyjournal.org/wound-healing-cell-viability-and-antimicrobial-potency-of-mucus-from-pangasianodon-hypophthalmus/ |
| work_keys_str_mv | AT mgomathy woundhealingcellviabilityandantimicrobialpotencyofmucusfrompangasianodonhypophthalmus AT manikantanpappuswamy woundhealingcellviabilityandantimicrobialpotencyofmucusfrompangasianodonhypophthalmus |