Amikacin-loaded selenium nanoparticles improved antibacterial and antibiofilm activity of amikacin against bovine mastitis-causing Staphylococcus aureus
Background: Antibiotic resistance in various microorganisms has become one of the most serious health problems worldwide. The use of nanoparticles in combination with conventional antibiotics is one of the recent efforts to overcome these challenges. This study aims to synthesize and evaluate the po...
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Elsevier
2025-01-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024171344 |
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| author | Leila Asadpour Maryam Alsadat Mehrbakhsh Bandari Roozbeh Sojoudi Masouleh |
| author_facet | Leila Asadpour Maryam Alsadat Mehrbakhsh Bandari Roozbeh Sojoudi Masouleh |
| author_sort | Leila Asadpour |
| collection | DOAJ |
| description | Background: Antibiotic resistance in various microorganisms has become one of the most serious health problems worldwide. The use of nanoparticles in combination with conventional antibiotics is one of the recent efforts to overcome these challenges. This study aims to synthesize and evaluate the possibility of using amikacin-loaded selenium nanoparticles as antibacterial agent against multidrug-resistant Staphylococcus aureus, that causes bovine mastitis. Methods: Selenium nanoparticles (SeNPs) were synthesized through chemical reduction of sodium selenite using L-cysteine. Loading of amikacin on selenium nanoparticles was done by mixing both in solution and confirmed by UV–Vis spectroscopy, XRD, SEM, and DLS. Antibacterial properties of obtained nanoparticles against S. aureus were determined using agar disc diffusion, broth micro dilution methods and also time-kill assay. Anti-biofilm properties of amikacin-loaded selenium nanoparticles was determined using microplate method and through determining the expression level of biofilm associated genes including icaA and icaD in S. aureus isolates treated with sub-MIC concentration of these nanoparticles by real-time PCR. Results: Synthetized SeNPs and amikacin-loaded selenium nanoparticles (SeNPs@AMK) exhibited spherical appearances and 80.4 % of Se° had a diameter of 120 nm. SeNPs and SeNPs@AMK exhibited antibacterial effects against S. aureus isolates at the range of 32–128 μg/mL and 1–32 μg/mL respectively. Dependent on concentration and the exposure time, bacterial killing was promoted by the SeNPs@AMK treatment. The use of SeNPs@AMK decreased the biofilm formation of the isolates by more than 50 % and also lead to down-regulation of icaA and icaD biofilm associated gene compared to the control. Conclusions: The results of this study suggest the antimicrobial properties of SeNPs and the reduction in the effective concentration of nanoparticle-loaded amikacin. Therefore, loading of antibiotics on the surface of nanoparticles may be used as a strategy to deal with the growing problem of drug resistance. |
| format | Article |
| id | doaj-art-be94ccccfed445bd89957e9c35f7cd6d |
| institution | DOAJ |
| issn | 2405-8440 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj-art-be94ccccfed445bd89957e9c35f7cd6d2025-08-20T02:46:36ZengElsevierHeliyon2405-84402025-01-01111e4110310.1016/j.heliyon.2024.e41103Amikacin-loaded selenium nanoparticles improved antibacterial and antibiofilm activity of amikacin against bovine mastitis-causing Staphylococcus aureusLeila Asadpour0Maryam Alsadat Mehrbakhsh Bandari1Roozbeh Sojoudi Masouleh2Corresponding author.; Department of Biology, Rasht Branch, Islamic Azad University, Rasht, IranDepartment of Biology, Rasht Branch, Islamic Azad University, Rasht, IranDepartment of Biology, Rasht Branch, Islamic Azad University, Rasht, IranBackground: Antibiotic resistance in various microorganisms has become one of the most serious health problems worldwide. The use of nanoparticles in combination with conventional antibiotics is one of the recent efforts to overcome these challenges. This study aims to synthesize and evaluate the possibility of using amikacin-loaded selenium nanoparticles as antibacterial agent against multidrug-resistant Staphylococcus aureus, that causes bovine mastitis. Methods: Selenium nanoparticles (SeNPs) were synthesized through chemical reduction of sodium selenite using L-cysteine. Loading of amikacin on selenium nanoparticles was done by mixing both in solution and confirmed by UV–Vis spectroscopy, XRD, SEM, and DLS. Antibacterial properties of obtained nanoparticles against S. aureus were determined using agar disc diffusion, broth micro dilution methods and also time-kill assay. Anti-biofilm properties of amikacin-loaded selenium nanoparticles was determined using microplate method and through determining the expression level of biofilm associated genes including icaA and icaD in S. aureus isolates treated with sub-MIC concentration of these nanoparticles by real-time PCR. Results: Synthetized SeNPs and amikacin-loaded selenium nanoparticles (SeNPs@AMK) exhibited spherical appearances and 80.4 % of Se° had a diameter of 120 nm. SeNPs and SeNPs@AMK exhibited antibacterial effects against S. aureus isolates at the range of 32–128 μg/mL and 1–32 μg/mL respectively. Dependent on concentration and the exposure time, bacterial killing was promoted by the SeNPs@AMK treatment. The use of SeNPs@AMK decreased the biofilm formation of the isolates by more than 50 % and also lead to down-regulation of icaA and icaD biofilm associated gene compared to the control. Conclusions: The results of this study suggest the antimicrobial properties of SeNPs and the reduction in the effective concentration of nanoparticle-loaded amikacin. Therefore, loading of antibiotics on the surface of nanoparticles may be used as a strategy to deal with the growing problem of drug resistance.http://www.sciencedirect.com/science/article/pii/S2405844024171344AmikacinSe nanoparticlesDrug resistanceS. aureus |
| spellingShingle | Leila Asadpour Maryam Alsadat Mehrbakhsh Bandari Roozbeh Sojoudi Masouleh Amikacin-loaded selenium nanoparticles improved antibacterial and antibiofilm activity of amikacin against bovine mastitis-causing Staphylococcus aureus Heliyon Amikacin Se nanoparticles Drug resistance S. aureus |
| title | Amikacin-loaded selenium nanoparticles improved antibacterial and antibiofilm activity of amikacin against bovine mastitis-causing Staphylococcus aureus |
| title_full | Amikacin-loaded selenium nanoparticles improved antibacterial and antibiofilm activity of amikacin against bovine mastitis-causing Staphylococcus aureus |
| title_fullStr | Amikacin-loaded selenium nanoparticles improved antibacterial and antibiofilm activity of amikacin against bovine mastitis-causing Staphylococcus aureus |
| title_full_unstemmed | Amikacin-loaded selenium nanoparticles improved antibacterial and antibiofilm activity of amikacin against bovine mastitis-causing Staphylococcus aureus |
| title_short | Amikacin-loaded selenium nanoparticles improved antibacterial and antibiofilm activity of amikacin against bovine mastitis-causing Staphylococcus aureus |
| title_sort | amikacin loaded selenium nanoparticles improved antibacterial and antibiofilm activity of amikacin against bovine mastitis causing staphylococcus aureus |
| topic | Amikacin Se nanoparticles Drug resistance S. aureus |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024171344 |
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