Bifidobacterium longum subsp. infantis CCFM1269 promotes intestinal barrier and release of IFN-β through TLR4-TRIF dependent way in growing mice
Bifidobacterium longum subsp. infantis is a commensal bacterium that predominates in the infant gut, playing a critical role in both preventing foreign infections and facilitating immune development. This study aimed to explore the effects of B. longum subsp. infantis supplementation on interferon-b...
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Tsinghua University Press
2025-01-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2024.9250025 |
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| author | Mengfan Ding Bo Yang Bowen Li Haiqin Chen Renqiang Yu Ross R. Paul Catherine Stanton Shilong Jiang Jianxin Zhao Wei Chen |
| author_facet | Mengfan Ding Bo Yang Bowen Li Haiqin Chen Renqiang Yu Ross R. Paul Catherine Stanton Shilong Jiang Jianxin Zhao Wei Chen |
| author_sort | Mengfan Ding |
| collection | DOAJ |
| description | Bifidobacterium longum subsp. infantis is a commensal bacterium that predominates in the infant gut, playing a critical role in both preventing foreign infections and facilitating immune development. This study aimed to explore the effects of B. longum subsp. infantis supplementation on interferon-beta (IFN-β) secretion and intestinal barrier improvement in growing mice. Female and male mice were orally administered either saline or B. longum subsp. infantis CCFM1269 or I5TI (1 × 109 CFU/mice per day, n = 8) from 1-week-age until 3-, 4-, and 5-week-age. RNA sequencing analysis revealed that CCFM1269 exhibited potential antiviral capacity through increasing 2’-5’ oligoadenylate synthetase (OAS). Additionally, CCFM1269 supplementation significantly increased colonic IFN-β levels which combined with OAS in 3-week-old female and male mice by activating the TLR4-TRIF-dependent signaling pathway. However, this effect was not observed in 4- and 5-week-old mice. Furthermore, both CCFM1269 were found to modulate the gut microbiota composition and enhance the intestinal barrier function in 3-, 4-, and 5-week-old mice. In summary, the results of this study suggested that B. longum subsp. infantis CCFM1269 promoting intestinal barrier and releasing IFN-β in growing mice was in a strain-specific and time-dependent manner. |
| format | Article |
| id | doaj-art-be8e84ab0f5c4704bcffd7f33c0bd927 |
| institution | DOAJ |
| issn | 2097-0765 2213-4530 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-be8e84ab0f5c4704bcffd7f33c0bd9272025-08-20T02:47:52ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302025-01-01141925002510.26599/FSHW.2024.9250025Bifidobacterium longum subsp. infantis CCFM1269 promotes intestinal barrier and release of IFN-β through TLR4-TRIF dependent way in growing miceMengfan Ding0Bo Yang1Bowen Li2Haiqin Chen3Renqiang Yu4Ross R. Paul5Catherine Stanton6Shilong Jiang7Jianxin Zhao8Wei Chen9State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, ChinaDepartment of Neonatology, Wuxi Maternity and Child Health Care Hospital, Affiliated Women’s Hospital of Jiangnan University, Wuxi 214122, ChinaInternational Joint Research Center for Probiotics & Gut Health, Jiangnan University, Wuxi 214122, ChinaInternational Joint Research Center for Probiotics & Gut Health, Jiangnan University, Wuxi 214122, ChinaNutrition and Metabolism Research Division, Innovation Center, Heilongjiang Feihe Dairy Co., Ltd., Beijing 100015, ChinaState Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, ChinaBifidobacterium longum subsp. infantis is a commensal bacterium that predominates in the infant gut, playing a critical role in both preventing foreign infections and facilitating immune development. This study aimed to explore the effects of B. longum subsp. infantis supplementation on interferon-beta (IFN-β) secretion and intestinal barrier improvement in growing mice. Female and male mice were orally administered either saline or B. longum subsp. infantis CCFM1269 or I5TI (1 × 109 CFU/mice per day, n = 8) from 1-week-age until 3-, 4-, and 5-week-age. RNA sequencing analysis revealed that CCFM1269 exhibited potential antiviral capacity through increasing 2’-5’ oligoadenylate synthetase (OAS). Additionally, CCFM1269 supplementation significantly increased colonic IFN-β levels which combined with OAS in 3-week-old female and male mice by activating the TLR4-TRIF-dependent signaling pathway. However, this effect was not observed in 4- and 5-week-old mice. Furthermore, both CCFM1269 were found to modulate the gut microbiota composition and enhance the intestinal barrier function in 3-, 4-, and 5-week-old mice. In summary, the results of this study suggested that B. longum subsp. infantis CCFM1269 promoting intestinal barrier and releasing IFN-β in growing mice was in a strain-specific and time-dependent manner.https://www.sciopen.com/article/10.26599/FSHW.2024.9250025bifidobacterium longum subsp. infantisintestinal barrierinterferon-beta (ifn-β) secretionage dependence |
| spellingShingle | Mengfan Ding Bo Yang Bowen Li Haiqin Chen Renqiang Yu Ross R. Paul Catherine Stanton Shilong Jiang Jianxin Zhao Wei Chen Bifidobacterium longum subsp. infantis CCFM1269 promotes intestinal barrier and release of IFN-β through TLR4-TRIF dependent way in growing mice Food Science and Human Wellness bifidobacterium longum subsp. infantis intestinal barrier interferon-beta (ifn-β) secretion age dependence |
| title | Bifidobacterium longum subsp. infantis CCFM1269 promotes intestinal barrier and release of IFN-β through TLR4-TRIF dependent way in growing mice |
| title_full | Bifidobacterium longum subsp. infantis CCFM1269 promotes intestinal barrier and release of IFN-β through TLR4-TRIF dependent way in growing mice |
| title_fullStr | Bifidobacterium longum subsp. infantis CCFM1269 promotes intestinal barrier and release of IFN-β through TLR4-TRIF dependent way in growing mice |
| title_full_unstemmed | Bifidobacterium longum subsp. infantis CCFM1269 promotes intestinal barrier and release of IFN-β through TLR4-TRIF dependent way in growing mice |
| title_short | Bifidobacterium longum subsp. infantis CCFM1269 promotes intestinal barrier and release of IFN-β through TLR4-TRIF dependent way in growing mice |
| title_sort | bifidobacterium longum subsp infantis ccfm1269 promotes intestinal barrier and release of ifn β through tlr4 trif dependent way in growing mice |
| topic | bifidobacterium longum subsp. infantis intestinal barrier interferon-beta (ifn-β) secretion age dependence |
| url | https://www.sciopen.com/article/10.26599/FSHW.2024.9250025 |
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